Method for enhancement of chemiluminescence

• US 4,927,769 A
• Filed: 07/08/1987
• Issued: 05/22/1990
• Est. Priority Date: 07/08/1987
• Status: Expired due to Term

• Current Assignee: CIBA Corning Diagnostics Corporation Medfield Massachusetts
• Original Assignee: CIBA Vision Corporation (Bayer AG)
• Inventors: Miller, Thomas E., Chang, Steve C. S.
• Primary Examiner(s): Kepplinger, Esther M.
• Assistant Examiner(s): Hoffer, Florina B.
• Application Number:
  US07/071,660
• Time in Patent Office: 1,049 Days
• Field of Search: 252/700, 435/8, 436/546, 436/800, 436/805, 436/172, 436/826, 436/518, 436/544, 436/543, 436/164, 530/802, 546/103
• US Class Current: 436/518
• CPC Class Codes: G01N 33/52 Use of compounds or compositions for colorimetric, spectrophotometric or fluorometric investigation, e.g. use of reagent paper and including single- and multilayer analytical elements immunological elements G01N33/54386; involving labelled immunochemicals G01N33/58; for haemoglobin or occult blood G01N33/72
• CPC Class Codes: G01N 33/582 with fluorescent label
• CPC Class Codes: Y10S 436/805 Optical property
• CPC Class Codes: Y10S 436/826 Additives, e.g. buffers, diluents, preservatives
• CPC Class Codes: Y10S 530/802 Chromogenic or luminescent peptides

Abstract

A method for enhancing the chemiluminescent signal of an acridinium ester in a chemiluminescent reaction which comprises oxidizing the acridinium ester in the presence of an enhancer selected from the group consisting of: (a) a cationic surfactant; (b) a nonionic surfactant; and (c) a sulfated primary alcohol.

Claims

1. A method for enhancing the chemiluminescent signal of an acridinium ester in a chemiluminescent reaction which comprises oxidizing the acridinium ester in the presence of an effective amount of an enhancer to effect enhanced chemiluminescent signal said enhancer selected from the group consisting of:

(a) a quarternary amine cationic surfactant of formula;

##STR5## wherein R¹ is C₈ -C₂₀ alkyl or alkenyl; and

R² R³ and R⁴ are C₁ -C₄ alkyl or alkenyl.

(b) a nonionic surfactant; and

(c) a sulfated primary alcohol selected from the group consisting of sodium dodecylsulfate, magnesium dodecylsulfate and tridecylsulfate.

2. A method as recited in claim 1 wherein the acridinium ester has the formula:

##STR6## where R₁ is alkyl, alkenyl, alkynyl, or aryl;

R₂, R₃, R₅, or R₇ are hydrogen, amino, carboxyl, hydroxyl, alkoxyl, nitro, or halide;

R₄ or R₈ are hydrogen, alkyl, alkenyl, alkynyl, aryl, alkoxyl, or amino,;

R₆ represents the following substituent;

space="preserve" listing-type="equation">R.sub.6 =--R.sub.9 --R.sub.10

where R₉ is omitted or is alkyl, aryl, or aralkyl linking groups, and R₁₀ is selected from the group consisting of;

##STR7## X is CH₃ SO4, OSO₂ F, halide, OSO₂ CF₃, OSO₂ C₄ F₉, or ##STR8## and R is alkyl, aryl, or alralkyl; and

finally R₅, and R₇ substituent positions on the phenoxy ring are interchangeable with the R₆ substituent position.

3. A method as recited in claim 2 werein R₄ and R₈ are alkyl, alkenyl, alkynyl, aryl, alkoxyl, or amino.

4. A method as recited in claim 2 wherein R₁ is methyl;

R₂, R₃, R₅, and R₇ are hydrogen;

R₄ and R₈ are methyl, R₁₀ is an N-succinimidyloxycarbonyl group attached directly to the para-position of the phenoxy ring, and X is CH₃ SO₄.

5. A method as recited in claim 1 wherein the effective amount of enhancer in the chemiluminescent reaction mixture is greater than 0.005% by weight.

6. A method as recited in claim 5 wherein the effective amount of enhancer in the chemiluminescent reaction mixture is greater than 0.01% by weight.

7. A method as recited in claim 1 wherein R¹ is C₁₄ -C₁₆ alkyl or alkenyl;

R², R³ and R⁴ are methyl or ethyl.

8. A method as recited in claim 7 wherein the cationic surfactant is hexadecyltrimethylammonium chloride.

9. A method as recited in claim 1 wherein the enhancer is a nonionic surfactant.

10. A method as recited in claim 9 wherein the nonionic surfactant is a polyoxyethylenated nonionic surfactant.

11. A method as recited in claim 1 wherein the sulfated primary alcohol is sodium dodecylsulfate.

12. In a chemiluscent immunoassasy utilizing an acridinium ester as a label wherein the improvement comprises oxidizing the acridinium ester in the presence of an effective amount of an enhancer selected from the group consisting of:

(a) a quarternary amine cationic surfactant of formula;

##STR9## wherein R¹ is C₈ -C₂₀ alkyl or alkenyl; and

R², R³ and R⁴ are C₁ -C₄ alkyl or alkenyl.

13. A chemiluminescent assay as recited in claim 12 wherein R¹ is C₁₄ -C₁₆ alkyl or alkenyl;

and R², R³ and R⁴ are methyl or ethyl.