Method for solid phase membrane mimetics

• US 4,927,879 A
• Filed: 10/24/1988
• Issued: 05/22/1990
• Est. Priority Date: 02/25/1988
• Status: Expired due to Term

• Current Assignee: Purdue Research Foundation (The Trustees of Purdue University (d/b/a Purdue University))
• Original Assignee: Purdue Research Foundation (The Trustees of Purdue University (d/b/a Purdue University))
• Inventors: Pidgeon, Charles
• Primary Examiner(s): Kight, III, John
• Assistant Examiner(s): Nutter, Nathan M.
• Application Number:
  US07/261,502
• Time in Patent Office: 575 Days
• Field of Search: 530/345, 530/811, 530/812, 530/813, 530/814, 530/815, 428/357, 428/402, 428/403, 428/406, 428/407, 210/656, 525/54.1
• US Class Current: 525/54.1
• CPC Class Codes: A61K 9/127 Liposomes
• CPC Class Codes: B01J 20/3204 Inorganic carriers, supports or substrates
• CPC Class Codes: B01J 20/3212 consisting of a polymer obtained by reactions otherwise than involving only carbon to carbon unsaturated bonds
• CPC Class Codes: B01J 20/3219 involving a particular spacer or linking group, e.g. for attaching an active group
• CPC Class Codes: B01J 20/3251 comprising at least two different types of heteroatoms selected from nitrogen, oxygen or sulphur
• CPC Class Codes: B01J 20/3274 Proteins, nucleic acids, polysaccharides, antibodies or antigens
• CPC Class Codes: B01J 20/3282 Crosslinked polymers
• CPC Class Codes: G01N 2030/527 sorbent material in form of a membrane
• CPC Class Codes: Y10S 530/811 Peptides or proteins is immobilized on, or in, an inorganic carrier
• CPC Class Codes: Y10S 530/812 Peptides or proteins is immobilized on, or in, an organic carrier
• CPC Class Codes: Y10S 530/813 Carrier is a saccharide
• CPC Class Codes: Y10S 530/814 Cellulose or derivatives thereof
• CPC Class Codes: Y10S 530/815 Carrier is a synthetic polymer
• CPC Class Codes: Y10T 428/2982 Particulate matter [e.g., sphere, flake, etc.]
• CPC Class Codes: Y10T 428/2991 Coated
• CPC Class Codes: Y10T 428/2996 Glass particles or spheres
• CPC Class Codes: Y10T 428/2998 including synthetic resin or polymer

Abstract

A membrane mimetic structure having a hydrophilic outer portion and a hydrophobic inner portion is covalently bound to a surface having reactive functional groups. The membrane mimetic structure consists essentially of adjacent amphiphilic molecules independently covalently bound to the surface. The structures can be applied to surfaces and should find application in a wide variety of disciplines requiring surfaces exhibiting properties of biological membranes.

Claims

1. A method for forming a membrane mimetic structure on a surface having covalently bound nucleophilic functional groups, said structure having a hydrophilic outer portion and a hydrophobic inner portion and consisting essentially of a monomolecular layer of adjacent amphiphilic molecules independently covalently bound to said surface through at least a portion of the nucleophilic functional groups, said method comprising the step of contacting said surface with a cyclic dicarboxylic anhydride of the formula ##STR6## wherein n is an integer from 8 to 14, said step being conducted under conditions conducive to the reaction of the nucleophilic group and said cyclic anhydride, said reaction being conducted until the covalently bound amphiphilic molecules sterically hinder further reaction with the nucleophilic groups on the surface.