Method for detecting cancerous tissue using visible native luminescence

• US 4,930,516 A
• Filed: 04/25/1988
• Issued: 06/05/1990
• Est. Priority Date: 11/13/1985
• Status: Expired due to Term

• Current Assignee: Laser Diagnostic Instruments, Inc. (Sensivida Medical Technologies, Inc.)
• Original Assignee: Laser Diagnostic Instruments, Inc. (Sensivida Medical Technologies, Inc.)
• Inventors: Alfano, Michele A., Alfano, Robert R.
• Primary Examiner(s): Smith, Ruth S.
• Application Number:
  US07/186,747
• Time in Patent Office: 771 Days
• Field of Search: 128/633, 128/634, 128/665, 128/303.1, 128/395, 128/398, 356/435
• US Class Current: 600/477
• CPC Class Codes: A61B 5/0059 using light, e.g. diagnosis by transillumination, diascopy, fluorescence A61B5/0093, A61B5/1455, A61B5/02416 and A61B5/0261 take precedence
• CPC Class Codes: Y10S 607/901 Cancer detection

Abstract

A method and apparatus for detecting the presence of cancerous tissue using visible luminescence. The tissue to be examined is excited with a beam of monochromatic light that causes the tissue to fluoresce over a spectrum of wavelengths. The intensity at which the excited tissue fluoresces can be measured either over a spectrum or at a predetermined number of preselected wavelengths. By determining the wavelength(s) at which maximum intensity(ies) are attained for the tissue in question and by comparing these peak wavelengths, either visually or electronically, to the peak wavelength(s) derived from a known non-cancerous tissue, or by comparing the spectrum of the excited tissue with the spectrum of a known noncancerous tissue one can determine the carcinomatoid status of the tissue in question. The invention is based on the discovery that the visible luminescence spectra for cancerous and non-cancerous tissue are substantially different and that the differences are such that visible luminescence from tissue can be used to detect the presence of cancer.

Claims

1. A method for determining if tissue is cancerous comprising:

a. exciting a tissue to be examined with a beam of light, and

b. comparing the visible native luminescence emitted from the tissue with the visible native luminescence emitted from tissue whose condition is known.

2. A method for determining if tissue is cancerous comprising:

a. exciting a tissue to be examined with a beam of light.

b. observing the visible native luminescence emitted from the tissue at least two wavelengths, and

c. determining if the tissue is cancerous in accordance with said measurements.

3. A method for detecting cancerous tissue comprising:

a. exciting a tissue to be examined with a beam of light that is substantially monochromatic, and then

b. comparing the resulting native luminescence spectrum with the native luminescence spectra for native normal and cancerous tissue.

4. A method for detecting cancerous tissue comprising:

a. exciting a tissue to be examined with a beam of light that is at least substantially monochromatic.

b. measuring the intensity of the visible native luminescence emitted from the tissue at least two wavelengths, and

c. determining if the tissue is cancerous in accordance with said measurements.

5. The method of claim 4 and wherein determining if cancerous tissue is present comprises determining the ratios in intensities at at least two wavelengths and then comparing the ratios with the ratios of intensities at the same wavelengths for normal tissue.

6. The method of claim 4 and wherein determining if cancerous tissue is present includes producing a signal corresponding to the ratio between the intensities at least two wavelengths and then displaying said signals.

7. The method of claim 4 and wherein determining if cancerous tissue is present comprises producing a signal corresponding to the difference in the intensity at at least two wavelengths.

8. The method of claim 4 and wherein determining if cancerous tissue is present comprises producing a signal corresponding to the difference in spectral profiles.

9. A method for detecting cancerous tissue comprising:

a. exciting a tissue to be examined with a beam of monochromatic light.

b. generating a luminescence spectrum native of light emitted by the tissue, and

c. comparing the luminescent spectrum with a luminescent spectrum for a known normal tissue.