Methods of using chemiluminescent 1,2-dioxetanes
First Claim
1. A process in which light of different wavelengths is simultaneously released from two or more enzymatically, chemically or electrochemically decomposable chemiluminescent 1,2-dioxetane compounds, at least one of said 1,2-dioxetane compounds being an enzymatically decomposable 1,2-dioxetane compound, said 1,2-dioxetane compounds being configured, by means of the inclusion of a different light emitting fluorophore in each of said 1,2-dioxetane compounds, to each emit upon decomposition light of said different wavelengths, which comprises decomposing each of said 1,2-dioxetane compounds by means of one of two or more different enzyme, chemical or electrochemical decomposing means, each of said decomposing means being specific to one of said 1,2-dioxetane compounds, at least one of said decomposing means being an enzyme decomposing means.
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Abstract
Processes are disclosed in which light of different wavelengths is simultaneously released from two or more enzymatically decomposable chemiluminescent 1,2-dioxetane compounds, said compounds being configured, by means of the inclusion of a different light emitting fluorophore in each of them, to each emit light of said different wavelengths, by decomposing each of said compounds by means of a different enzyme. Such processes can be used in multi-channel assays--immunoassays, chemical assays and nucleic acid probe assays--to detect the presence or determine the concentration of chemical or biological substances, and in multi-channel chemical/physical probe procedures for studying the microstructures of macromolecules.
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19 Claims
- 1. A process in which light of different wavelengths is simultaneously released from two or more enzymatically, chemically or electrochemically decomposable chemiluminescent 1,2-dioxetane compounds, at least one of said 1,2-dioxetane compounds being an enzymatically decomposable 1,2-dioxetane compound, said 1,2-dioxetane compounds being configured, by means of the inclusion of a different light emitting fluorophore in each of said 1,2-dioxetane compounds, to each emit upon decomposition light of said different wavelengths, which comprises decomposing each of said 1,2-dioxetane compounds by means of one of two or more different enzyme, chemical or electrochemical decomposing means, each of said decomposing means being specific to one of said 1,2-dioxetane compounds, at least one of said decomposing means being an enzyme decomposing means.
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