Method and device for ketone measurement

• US 4,931,404 A
• Filed: 12/22/1986
• Issued: 06/05/1990
• Est. Priority Date: 12/22/1986
• Status: Expired due to Fees

• Current Assignee: Abbott Laboratories Incorporated
• Original Assignee: Abbott Laboratories Incorporated
• Inventors: Kundu, Samar K.
• Primary Examiner(s): Warden, Robert J.
• Assistant Examiner(s): Johnston, Jill
• Application Number:
  US06/944,793
• Time in Patent Office: 1,261 Days
• Field of Search: 436/128, 436/130, 422/58, 422/61, 422/85, 422/86
• US Class Current: 436/128
• CPC Class Codes: G01N 33/64 involving ketones
• CPC Class Codes: Y10T 436/200833 Carbonyl, ether, aldehyde or ketone containing
• CPC Class Codes: Y10T 436/202499 Formaldehyde or acetone

Abstract

The present invention relates to methods and materials for the detection of ketone and aldehyde analytes in fluid samples by means of reacting analyte containing samples with a matrix comprising a hydrazine compound coupled to an H⁺ ion exchange resin to produce a hydrazone reaction product with a characteristic color. Methods are also provided for ascertaining the fat catabolism effects of a weight loss dietary regimen comprising determining the breath acetone concentration of the subject by means of contacting said breath with a device comprising an H⁺ ion exchange resin to which a hydrazine compound has been coupled and correlating the breath acetone concentration to a standard reflecting the effect on breath acetone of fixed rates of fat metabolism.

Claims

1. A method for the detection of ketone and aldehyde analytes in a fluid sample comprising:

(a) reacting said analytes with a hydrazine compound coupled to an H⁺ ion exchange resin to form a colored reaction product,

(b) fluting said reaction product from the ion exchange resin with a solvent, and

(c) detecting the presence of a color signal in the eluted material.

2. The method according to claim 1 wherein the hydrazine compound is 2,4-dinitrophenyl hydrazine.

3. The method according to claim 1 wherein the H⁺ ion exchange resin is a strongly acidic polymeric particle ion exchange resin.

4. The method according to claim 1 wherein the fluid sample is a vapor.

5. The method according to claim 4 wherein the fluid sample is a breath sample.

6. The method according to claim 1 wherein the fluid sample is a liquid.

7. The method according to claim 1 wherein the analyte is acetone.

8. The method according to claim 1 wherein the fluid sample is preconcentrated onto an adsorbent material.

9. The method according to claim 8 wherein the adsorbent material is selected from the group consisting of 2,6-diphenyl-p-phenylene oxide polymers and activated silica.

10. The method according to claim 1 wherein the solvent is selected from the group consisting of lower alcohols.

11. The method according to claim 10 wherein the solvent is methanol.

12. A device for the detection of ketone and alkehyde analytes in a fluid sample comprising an H⁺ ion exchange resin to which a hydrazine compound has been coupled, and an adsorbent material.

13. A method for ascertaining the fat catabolism effects of a weight loss dietary regimen, said method comprising:

(a) determining the breath acetone concentration of the subject by (i) contacting said breath with a device comprising an H⁺ ion exchange resin to which a hydrazine compound has been coupled to form a colored reaction product, (ii) eluting said reaction product from the ion exchange resin with a solvent, and (iii) detecting the presence of a color, and

(b) correlating the breath acetone concentration to a standard reflecting the effect on breath acetone of fixed rates of fat catabolism.

14. A kit for the detection of ketone and aldehyde analytes in a fluid sample comprising:

(a) an H⁺ ion exchange resin to which a hydrazine compound has been coupled;

(b) a solid adsorbent;

(c) solvent means for eluting a reaction product from the H⁺ ion exchange resin; and

(d) means for detecting the presence of a color signal.

15. The kit according to claim 14 wherein the hydrazine compound is a 2,4-dinitrophenyl hydrazine.

16. A kit for ascertaining the fat catabolism effects of a weight loss dietary regimen comprising:

(a) means for collecting a fixed volume of breath and contacting it with the solid adsorbent;

(b) an H⁺ ion exchange resin to which 2,4-dinitrophenyl hydrazine has been coupled;

(c) a solid adsorbent;

(d) means for eluting said reaction

(e) means for detecting the presence of a color signal; and

(f) a standard reflecting the effect on breath acetone of fixed rates of fat catabolism.