Tissue analyzer

• US 4,932,044 A
• Filed: 11/04/1988
• Issued: 06/05/1990
• Est. Priority Date: 11/04/1988
• Status: Expired due to Fees

• Current Assignee: Yale University
• Original Assignee: Yale University
• Inventors: Williams, Robert W., Rakic, Pasko
• Primary Examiner(s): Heyman, John S.
• Application Number:
  US07/267,222
• Time in Patent Office: 578 Days
• Field of Search: 377/10, 377/112, 350/507, 350/529, 350/530, 382/6
• US Class Current: 377/10
• CPC Class Codes: G01N 15/1433 using image recognition
• CPC Class Codes: G01N 15/1468 with spatial resolution of the texture or inner structure of the particle
• CPC Class Codes: G06T 2207/30024 Cell structures in vitro; Tissue sections in vitro
• CPC Class Codes: G06T 7/0012 Biomedical image inspection

Abstract

A system is disclosed for counting particles/cells within a counting box of precisely known volume that is completely inside a transparent section or sample. The box has a chosen height with defined upper and lower limits and appropriately selected width and depth dimensions. It resides completely within the sample and has no surface in common with an exterior surface of the sample. The system includes a compound light microscope that has a depth of focus which is small in relation to the thickness dimension of the counting box. The microscope includes adjustment means for moving the focal plane through a range which is greater than the height of the counting box. Display means are provided which show the portion of the sample that is within the depth of focus and user-operated means is provided to enable the user to mark the cells so displayed. Indicator means are further provided to either audibly or visually indicate to the user when the adjustment means cause the focal plane to pass beyond either the upper or lower height limits of the counting box. The indicator means further includes means for accumulating a count of cells within the counting volume as the user operates the marker means. Means are also provided to compensate for optical foreshortening.

Claims

1. In a system for counting particles within an interior volume of a transparent sample, said volume having chosen thickness and width dimensions, said thickness dimension exhibiting upper and lower height limits, said interior volume containing no surface coincident with an exterior surface of said sample, the combination comprising:

a compound light microscope with a focal plane whose depth of field is small in relation to said thickness dimension, said microscope having adjustment means for moving said sample through a range greater than said thickness dimension;

display means for showing particles in said sample which are within said focal plane;

marker means for a marking a particle to be counted;

indicator means for indicating when said adjustment means causes said focal plane to overlap an upper or lower height limit; and

means for accumulating a count of marked particles.

2. The system as recite din claim 1 wherein said compound light microscope is provided with differential interference contrast optics to enhance the particles which are within said focal plane.

3. The system of claim 1 further comprising:

means for altering the showing of said display means when said adjustment means is operated to cause said focal plane to overlap an upper or lower height limit.

4. The system of claim 3 wherein said altering means modifies said display means in a first fashion when said overlap of said upper height limit occurs and modifies said display means in a second fashion when said overlap of said lower limit occurs.

5. The system of claim 4 wherein said first fashion causes said display means to exhibit a first color and said second fashion a second color.

6. The system of claim 1 wherein said indicator means comprises alarm means which provides audible tones when said adjustment means is operated so as to cause said focal plane to overlap an upper or lower height limit.

7. The system of claim 6 wherein said alarm means provides a first audible tone when said overlap of said upper limit occurs and a different audible tone when said overlap of said lower limit occurs.