Apparatus for mounting a diffusion-limiting membrane for a sensor

• US 4,933,066 A
• Filed: 10/16/1989
• Issued: 06/12/1990
• Est. Priority Date: 03/31/1987
• Status: Expired due to Term

• Current Assignee: Horiba Limited
• Original Assignee: Daikin Industries Ltd
• Inventors: Yamasaki, Koichi, Mukai, Toji, Osaka, Tatsuhiko, Terawaki, Hiroshi
• Primary Examiner(s): Kaplan, G. L.
• Application Number:
  US07/423,567
• Time in Patent Office: 239 Days
• Field of Search: 204/403, 204/1 E, 204/407, 204/402, 204/415, 435/817, 435/291, 435/288
• US Class Current: 204/403.05
• CPC Class Codes: C12Q 1/001 Enzyme electrodes
• CPC Class Codes: G01N 27/40 Semi-permeable membranes or partitions
• CPC Class Codes: Y10S 435/817 Enzyme or microbe electrode

Abstract

An apparatus for mounting a diffusion-limiting membrane for a sensor in which a mounting member for mounting a diffusion-limiting membrane holding plate is movable toward and away from an enzyme electrode unit and in which, with the mounting member positioned away from the enzyme electrode unit, a diffusion-limiting membrane holding plate having a diffusion-limiting membrane mounted thereon is mounted on or removed from the mounting member, thus preventing the diffusion-limiting membrane from being slidingly rubbed with the surface of the enzyme electrode and in which, with the holding plate mounted on the mounting member, the mounting member ius moved toward the enzyme electrode unit, causing the diffusion-limiting membrane to be stuck to the surface of the enzyme electrode unit.

Claims

1. An apparatus for mounting a diffusion-limiting membrane for a sensor comprising:

a pair of positioning portions on a sensor having an enzyme electrode unit, said sensor also having an enzyme-immobilized membrane, said pair of positioning portions being opposite to each other with respect to said enzyme electrode unit;

a resilient thin plate having first and second engagement means for engagement with said pair of positioning portions, said first and second engagement means being spaced further apart than said pair of positioning portions so as to cause said resilient thin plate to be resiliently bent upon engagement of said engagement means with said positioning portions;

said resilient thin plate having an opening formed therein in a position which is adapted to be opposite to said enzyme electrode unit; and

said opening being covered by a diffusion-limiting membrane secured to said resilient plate.

2. An apparatus for mounting a diffusion-limiting membrane for a sensor as set forth in claim 1, wherein the pair of positioning portions are immovably attached to the sensor.

3. An apparatus for mounting a diffusion-limiting membrane for a sensor comprising:

a mounting member for mounting a diffusion-limiting membrane holding plate;

said mounting member having a first end, a second end, an upper surface, a bottom surface and a through-hole which extends through said upper and bottom surfaces and which is adapted to house an enzyme electrode unit for measuring a target solution disposed on a sensor at a predetermined position thereof, said mounting member having a groove formed therein for housing a diffusion-limiting membrane holding plate, said groove extending to said first end of said mounting member so as to form an opening in said mounting member which enables the housing plate to extend away from said groove; and

said mounting member being movable in both directions toward and away from said electrode unit; and

positioning means for positioning said mounting member at a first position wherein said enzyme electrode is housed within the through-hole of said mounting member and at a second position wherein said mounting member is positioned away from said enzyme electrode unit.

4. An apparatus for mounting a diffusion-limiting membrane for a sensor as set forth in claim 3, wherein the mounting member for mounting a diffusion-limiting membrane holding plate is rotatable with one end thereof serving as a fulcrum.

5. An apparatus for mounting a diffusion-limiting membrane for a sensor as set forth in claim 3, wherein the positioning member is constituted by a swing member having a guide groove engaged with an engagement shaft disposed on the mounting member for mounting a diffusion-limiting membrane holding plate, said guide groove having a pair of engagement portions aligned with the swing center of said swing member.

6. An apparatus for mounting a diffusion-limiting membrane for a sensor as set forth in claim 5, further comprising:

an openable outer cover covering the top of the mounting member for mounting a diffusion-limiting membrane holding plate; and

positioning release means for releasing a state where said mounting member is positioned at the moved position in a direction toward the enzyme electrode unit, in association with the operation of closing said outer cover, said state having been provided by the said positioning means.

7. An apparatus for mounting a diffusion-limiting membrane for a sensor as set forth in claim 6, wherein, at the final stage of the operation of closing the outer cover, the positioning release means forcibly moves the mounting member to disengage the engagement shaft from one of the engagement portions, said one engagement portion being one with which said engagement shaft is engaged when said mounting member is moved in close to the enzyme electrode unit.

8. An apparatus for mounting a diffusion-limiting membrane for a sensor as set forth in claim 6, wherein, by sliding the positioning release means, the closed state of the outer cover is released and the swing member is forcibly swung to disengage the engagement shaft from one of the engagement portions, said one engagement portion being one with which said engagement shaft is engaged when said mounting member is moved in close to the enzyme electrode unit.

9. An apparatus for mounting a diffusion-limiting membrane for a sensor as set forth in claim 3, further comprising an insertion/removal preventive means adapted to be engaged with the diffusion-limiting membrane holding plate when the mounting member for mounting a diffusion-limiting membrane holding plate is moved toward the enzyme electrode unit.

10. An apparatus for mounting a diffusion-limiting membrane for a sensor as set forth in claim 9, wherein the mounting member for mounting a diffusion-limiting membrane holding plate is further provided in the groove thereof with a through-hole, and the insertion/removal preventive means is a pulling preventive shaft disposed on the sensor such that said shaft is adapted to project on said groove through said through-hole when said mounting member is moved toward the enzyme electrode unit.

11. An apparatus for mounting a diffusion-limiting membrane for a sensor as set forth in claim 3, further comprising a release mechanism for releasing a moving range limiting state provided by the positioning member.

12. An apparatus for mounting a diffusion-limiting membrane for a sensor as set forth in claim 11, wherein the positioning member is a swing member having a guide groove engaged with an engagement shaft disposed on the mounting member for mounting a diffusion-limiting membrane holding plate, said guide groove having a pair of engagement portions aligned with the swing center of said swing member, and

the release mechanism is adapted to move said swing member in such direction that said engagement shaft is disengaged from said guide groove.

13. An apparatus for mounting a diffusion-limiting membrane for a sensor as set forth in claim 12, further comprising a lever normally biased toward the engagement shaft, said lever having the swing member.

14. An apparatus for mounting a diffusion-limiting membrane for a sensor as set forth in claim 11, wherein the release mechanism is operable from the outside of the lower, surface of the sensor.

15. An apparatus for mounting a diffusion-limiting membrane for a sensor as set forth in claim 3, further comprising:

an outer cover to be opened and closed, said outer cover being positioned so as to be opposite to the enzyme electrode unit when said cover is closed; and

an inner cover adapted to be moved in association with the operation of opening/closing said outer cover,

said inner cover being adapted to cover at least a portion of said enzyme electrode unit when said outer cover is opened.

16. An apparatus for mounting a diffusion-limiting membrane for a sensor as set forth in claim 15, wherein the inner cover is further provided with a large-diameter opening adapted to be opposite to the enzyme electrode unit when the outer cover is closed, and

the outer cover is further provided with a wetting liquid holding portion adapted to come in contact with said enzyme electrode unit when the outer cover is closed.

17. An apparatus for mounting a diffusion-limiting membrane for a sensor as set forth in claim 15, wherein the outer cover is rotatable with respect to the sensor and the inner cover is slidable with respect to the sensor, and wherein there is further disposed links for connecting said outer cover to said inner cover

18. An apparatus for mounting a diffusion-limiting membrane for a sensor as set forth in claim 17, wherein the mounting member for mounting a diffusion-limiting membrane holding plate is further provided with guide grooves, and the inner cover is further provided with projections engaged with said guide grooves, said projections being connected to said outer cover with the links.

19. An apparatus for mounting a diffusion-limiting membrane for a sensor as set forth in claim 15, further comprising a regulating mechanism permitting the inner cover to be moved in a direction away from the mounting member for mounting a diffusion-limiting membrane holding plate, only when said inner cover receives an upwardly moving force greater than a predetermined amount.

20. An apparatus for mounting a diffusion-limiting membrane for a sensor as set forth in claim 19, wherein the regulating mechanism is constituted by projections which are projectingly formed on the inner cover at predetermined positions thereof.

21. An apparatus for mounting a diffusion-limiting membrane for a sensor as set forth in claim 19, wherein the regulating mechanism is constituted by projections which are projectingly formed on the sensor at predetermined positions thereof.

22. An apparatus for mounting a diffusion-limiting membrane for a sensor as set forth in claim 19, wherein the regulating mechanism permits the inner cover to be sufficiently moved upward only when said inner cover directly receives an upwardly rotating force greater than a predetermined amount.

23. An apparatus for mounting a diffusion-limiting membrane for a sensor as set forth in claim 19, wherein the regulating mechanism permits the inner cover to be sufficiently moved upward only when the outer cover is opened by an angle exceeding a predetermined angle.

24. An apparatus for mounting a diffusion-limiting membrane for a sensor comprising:

a pair of positioning portions disposed on a sensor having an enzyme electrode unit, said sensor also having an enzyme-immobilized membrane, said pair of positioning portions being opposite to each other with respect to said enzyme electrode unit;

a resilient thin plate adapted to be engaged with said pair of positioning portions, causing said resilient thin plate to resiliently bend to conform to the curvature of said enzyme electrode unit;

said resilient thin plate having an opening in the position thereof which is adapted to be opposite to said enzyme electrode unit; and

said opening being covered by a diffusion-limiting membrane secured to said resilient thin plate and one of said pair of positioning portions being immovably attached to the sensor, while the other of said pair of positioning portions is vertically movably attached to the sensor.