Methods of operating enzyme electrode sensors
First Claim
1. A method of determining the concentration of an analyte in a sample by reacting the analyte with an enzyme on a sensor incorporating a previously unused electrode to produce an electrochemically active species and detecting that species by means of the sensor, comprising:
- (a) connecting said previously unused electrode in an electrical circuit, and applying a potential to the sensor to cause an electric current to flow therethrough;
(b) applying the sample to the sensor;
(c) reducing the applied potential to zero;
(d) increasing the applied potential to a value in the range 0.8 to 1.9 volts;
(e) maintaining the increased potential for a period in the range of 15 seconds to 1 minute;
(f) reducing the applied potential directly to a value in the range 0.4 to 0.75 volts;
(g) determining the concentration of the analyte at said reduced potential; and
(h) discarding the electrode after use.
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Abstract
An analytical method using a sensor of the enzyme electrode type is provided for determining in a sample the concentration of an analyte reactive with an enzyme present on the sensor to produce an electrochemically active species detectable by the sensor, which comprises applying a potential across the sensor to cause an electrical current to flow therethrough, contacting the sensor with the sample thereby causing a change in the current flow and, when a working potential is applied across the sensor, determining the concentration of the analyte as a function of the change in the current characterized in that at some time before the working potential is applied and the concentration of the analyte is determined a potential significantly in excess of the working potential is applied, whereby to reduce the period required to establish a signal sufficiently stable to permit the determination to be effected.
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Citations
10 Claims
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1. A method of determining the concentration of an analyte in a sample by reacting the analyte with an enzyme on a sensor incorporating a previously unused electrode to produce an electrochemically active species and detecting that species by means of the sensor, comprising:
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(a) connecting said previously unused electrode in an electrical circuit, and applying a potential to the sensor to cause an electric current to flow therethrough; (b) applying the sample to the sensor; (c) reducing the applied potential to zero; (d) increasing the applied potential to a value in the range 0.8 to 1.9 volts; (e) maintaining the increased potential for a period in the range of 15 seconds to 1 minute; (f) reducing the applied potential directly to a value in the range 0.4 to 0.75 volts; (g) determining the concentration of the analyte at said reduced potential; and (h) discarding the electrode after use. - View Dependent Claims (2)
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3. A method for determining the concentration of an analyte in a sample by reacting the analyte with an enzyme on a sensor to produce an electrochemically active species and detecting that species by means of the sensor, wherein the sensor incorporates an electrode which is initially not electrically connected, comprising the steps of:
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(a) applying a potential to the sensor to cause an electric current to flow therethrough; (b) applying the sample to the sensor, whereby to cause a change in said electric current; (c) in response to said change in said electric current, increasing the potential applied to the sensor to a significantly increased level, for a period of time sufficient to establish a stable signal; (d) significantly reducing the potential applied to the sensor directly to a predetermined working potential; and (e) determining the concentration of the analyte. - View Dependent Claims (4, 5, 6, 7, 8)
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9. A method for determining the concentration of an analyte in a sample by reacting the analyte with an enzyme on a sensor to produce an electro-chemically active species and detecting that species by means of the sensor, wherein the sensor incorporates an electrode which is initially not electrically connected, comprising the steps of:
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(a) applying to the sensor a potential at a level significantly in excess of a predetermined working potential and thereby cause an electric current to flow through said sensor; (b) applying the sample to the sensor and thereby causing a change in said electric current; (c) in response to said change in said electric current, maintaining said potential at said level for a period of time sufficient to establish a stable signal; (d) significantly directly reducing the potential applied to the sensor to said predetermined working potential; and (e) determining the concentration of the analyte.
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10. A method of determining the concentration of an analyte in a sample by reacting the analyte with an enzyme on a sensor incorporating a previously unused electrode to produce an electrochemically active species and detecting that species by means of the sensor, comprising:
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(a) connecting said previously unused electrode in an electrical circuit and applying to the sensor a potential to cause an electric current to flow therethrough; (b) applying the sample to the sensor;
increasing the potential to a value significantly in excess of a predetermined working potential;(c) maintaining said increased potential for a length of time sufficient to establish a stable signal; (d) reducing said potential to said predetermined working potential; (e) determining the concentration of the analyte at said working potential; and (f) discarding the electrode.
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Specification