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Methods of operating enzyme electrode sensors

  • US 4,935,105 A
  • Filed: 10/18/1989
  • Issued: 06/19/1990
  • Est. Priority Date: 02/24/1987
  • Status: Expired due to Fees
First Claim
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1. A method of determining the concentration of an analyte in a sample by reacting the analyte with an enzyme on a sensor incorporating a previously unused electrode to produce an electrochemically active species and detecting that species by means of the sensor, comprising:

  • (a) connecting said previously unused electrode in an electrical circuit, and applying a potential to the sensor to cause an electric current to flow therethrough;

    (b) applying the sample to the sensor;

    (c) reducing the applied potential to zero;

    (d) increasing the applied potential to a value in the range 0.8 to 1.9 volts;

    (e) maintaining the increased potential for a period in the range of 15 seconds to 1 minute;

    (f) reducing the applied potential directly to a value in the range 0.4 to 0.75 volts;

    (g) determining the concentration of the analyte at said reduced potential; and

    (h) discarding the electrode after use.

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