Method and apparatus for fast, reliable, and environmentally safe dispensing of fluids, gases and individual particles of a suspension through pressure control at well defined parts of a closed flow-through system

• US 4,936,465 A
• Filed: 09/15/1989
• Issued: 06/26/1990
• Est. Priority Date: 12/07/1987
• Status: Expired due to Fees

• Current Assignee: Tibor Zold
• Original Assignee: Tibor Zold
• Inventors: Zold, Tibor
• Primary Examiner(s): Hajec, Donald T.
• Application Number:
  US07/408,392
• Time in Patent Office: 284 Days
• Field of Search: 209/3.1, 209/3.2, 209/3.3, 209/606, 209/552, 209/643, 209/644, 209/932, 209/942, 209/906, 356/39, 356/335, 324/71.4, 222/420-422
• US Class Current: 209/3.1
• CPC Class Codes: B07C 3/02 Apparatus characterised by the means used for distribution
• CPC Class Codes: G01N 15/149 specially adapted for sorting particles, e.g. by their size or optical properties
• CPC Class Codes: Y10S 209/906 Pneumatic or liquid stream feeding item

Abstract

A method and apparatus for controlling flows of fluids, suspensions, gases, aerosols, and for sorting individual particles, flowing in transporter ducts of a flow-through duct system, using for control of the flow and hence, for dispensing and sorting processes, a pressure increase for a short time interval, at the entrances of preselected ducts having a value so that the flow of the fluid or gas to be dispensed comes to a stop at these entrances and is thus constrained together with the particle to flow into ducts where the pressure increase does not take place during the same dispensing/sorting phase. The momentary pressure increase is caused at the ducts either by injection of another fluid or gas through the injection duct, corresponding to these ducts, or by generation of a gas bubble or vapor between electrodes situated at the entrance of these ducts. Buffer cavities, situated between the fast fluctuating part of the system and already processed fluid, itself flowing slowly in the drainage tubes, assure very fast, reliable and stable operation for dispenser/sorter system since through the buffering effect, each dispensing flow configuration is independent from previous configurations. The density of the processed suspension is increased during processing inside the system by special filtering/collector cavities of the same kind. The possible uses of the invention include cell sorting in the fields of cytology and cancer research, accurate, environmentally friendly dispensing of any fluid or gas, i.e., in pharmaceutical chemistry, combustion research and the distribution of very dangerous fluids and gases.

Claims

1. A method for fast and reliable control of the flow of fluid substances and fluid volume elements carrying individual particles of a suspension, flowing in a hole-like, environmentally friendly, duct flow system having inlets and outlets, under the effect of pressure differential existing between the inlets and outlets of the system, the duct flow system having one mode of operation in which control of the flow of the fluids is used for dispensing the fluid substances through a number of outlets of the system with a preselected dispensing format, and a second mode of operation in which control of the flow of the fluid substance is used for dispensing volume elements, containing at least one particle, in accordance with at least one individual physio-chemical property of the particles, so as to sort these particles out of their original suspension into a plurality of partial suspensions, and where a combined multi-parameter compensating process assures fast and reliable achievement of each of a number of preselected flow configurations, defined by the distribution of the flow of the substance in the various outlets of the system, whereby through said compensating process, each of the flow configurations is achieved independently from the other flow configurations during the whole operation of the system, wherein said method comprises the steps of:

(a) transporting said fluid substances in at least one transporting duct towards a duct branching zone [while analyzing the individual particles in the ducts, in the second mode, in accordance with at least one of their properties], and subsequently determining a flow configuration of the substance in the various outlets of the flow system [while, in the first mode, the flow configuration is determined without analyzing the particles] when said system is in said first mode without analyzing said particles, and when in said second mode analyzing for at least one of their properties;

(b) creating a corresponding flow configuration for said duct branching zone, where not only at least one transporting duct enters but also a multitude of dispensing ducts branch out radially in space, by discontinuously injecting a fluid control medium, at the entrance of a number of dispensing ducts of the multitude of dispensing ducts where the substances to be dispensed are not to enter, with a force and at a place of injection so that the substances are forced to enter into other dispensing ducts of the multitude of dispensing ducts, while the control medium flows into that duct into which it was injected, the discontinuous injecting causing a small mass of the control medium and the substances to rapidly fluctuate;

(c) providing a buffer cavity at the end of each dispensing duct;

(d) transporting the dispensed substances in their dispensing ducts toward respective corresponding buffer cavities, and transporting the control medium in its ducts towards buffer cavities corresponding to the control medium ducts;

(e) decoupling the rapidly fluctuating small mass of the substances and the control medium, flowing from the dispensing ducts into the corresponding buffer cavities, from the fluid flowing in the dispensing ducts, which is of a larger mass or amount which slowly out of the buffer cavities since each of these cavities has a fluid-free volume which is substantially larger than that of the whole duct flow system, whereby a very fast fluctuation of the small amount of the substances to be dispensed is possible and from where the very fast operation of the system also follows;

(f) buffering, simultaneously in the buffer cavities, the pressure, which is caused partly by a fluctuating influx of the control medium into these cavities, and selectably separating the control medium from the processed substance when the control medium is of a gaseous nature and the processed substance is of a liquid nature by forcing the gaseous control medium, through the effect of buoyancy, to leave the cavities through a gas outlet hole, situated at the upper part of the cavity, and simultaneously letting the dispensed substance leave the cavity through an outlet hole, situated at the bottom of the cavity, and causing, through this type of buffering, the exact return of the pressure and flow conditions in the dispensing part of the system to a steady flow state where no control medium is injected, whereafter, the system is ready for another prescribed and completely independent dispensing phase; and

(g) directing the dispensed fluids through a two-position multiple-tube value selectively leading to corresponding filtering/collector means for separating the sorted particles from most of the suspending fluid, so that a very highly concentrated suspension is obtained, and into a waste container, the obtained end result then being transferred manually to other containers.

2. The method according to claim 1, wherein the pressure difference causing the flow in the system, is created by applying a higher than atmospheric pessure at the inlets of the system, while the outlets of the system remain at atmospheric pressure.

3. The method according to claim 1, wherein the pressure difference causing the flow in the system, is created by applying a vacuum at the outlets of the system, while the inlets of the system remain at atmospheric pressure.

4. The method according to claim 1, wherein the pressure difference causing the flow in the system, is created by applying a higher than atmospheric pressure at the inlets of the system, and applying a vacuum at some of the outlets of the system, the remaining outlets being left at some other pressure.

5. The method according to claim 1, wherein the fluid substance occupies the whole crosssection of some of the transporting ducts and in other transporting ducts the flow geometry is of another form, the transporting ducts being entrance ducts of the flow system.

6. The method according to claim 1, wherein more than one substance occupies some of the transporting ducts, which substances flow quasi-laminarly beside each other in each of the ducts, said quasi-laminar flows being arranged in a flow chamber attached to the entrance of each of the transporting ducts.

7. The method according to claim 1, wherein at least one thin stream flows in some of the transporting ducts, this at least one stream being enveloped by a sheath stream which keeps the thin stream at a stable position as it flows in the transport duct, said flow being created in a nozzle chamber.

8. The method according to claim 7, wherein said thin streams, in a steady flow state, flow into the same dispenser duct while in the dispensing phase, they flow into the preselected dispenser ducts.

9. The method according to claim 7, wherein said streams have a cross-section so as to orient the particles, carried by said streams, into a proper direction relative to the cross-section of the corresponding transporting duct so as to obtain optimal analyzing conditions for the particles.

10. The method according to claim 1, wherein places of particle analysis are only located at a distance from the entrance of the duct branching zone so that the flow-through time of a particle between these two places is not shorter than the time necessary to establish the predetermined dispensing flow configuration for sorting of the particles.

11. The method according to claim 3, wherein the transporting channels have a hydrodynamic resistance which is as large as possible.

12. The method according to claim 1, wherein the control medium is injected into the entrance of the duct at an optimal place so that the amount of the substance to be processed has a minimum value that has to be redirected during establishment of the required flow configuration, the place of the injection being slightly inside of the duct branching zone and partly in the corresponding dispenser duct.

13. The method according to claim 12, wherein the control medium is injected into said optimal place through a control hole which communicates with the outside of the system.

14. The method according to claim 12, wherein the control medium is a vapor or gas.

15. The method according to claim 1, wherein the dispenser ducts have a minimum length so that the control medium does not reach the end of these ducts during its injection therein, so that dispensing is not disturbed by a possible pressure varation caused by the entrance of the control medium into the corresponding buffering cavity.

56. A method according to claim 1, wherein the flow of fluid substances includes one of the fluids, gases, and aerosols.

57. A method according to claim 1, wherein the fluid control medium is one of a fluid and a gas.

58. A method according to claim 4, wherein the remaining outlets are left at atmospheric pressure.

59. A method according to claim 7, wherein said thin streams, in a steady flow state, flow into different dispenser ducts, while in the dispensing phase, they flow into the preselected dispenser ducts.

70. A method according to claim 1, wherein a first electronic system determines the proper dispensing flow configuration, and a second electronic system determines the proper flow configuration.

16. A dispensing and sorting device for a fast and reliable control of a flow of fluid substances and volume elements containing individual particles in suspension, in a closed, hole-like, environmentally friendly, duct system having inlets and outlets under the effect of a pressure difference, existing between the inlets and the outlets, the control of the flow, in a dispensing mode, being useable for dispensing the fluid substances through a plurality of outlets of the duct system in accordance with a preselected dispensing format, or in a particle sorting mode, for dispensing volume elements of the suspension, containing at least one particle, in accordance with at least one individual physio-chemical property of the particles, so as to sort these particles out of their original suspension into a number of groups, and where a combined compensating process assures not only a fast and reliable achievement of each individual, preselected flow configuration, differing from other flow configurations in that aspect in which dispenser ducts the just dispensed substance flows, but also a fast and exact return of the flow to its steady state flow configuration after each such dispensing phase during the whole operation of the system, whereby one dispensing or sorting phase is completely independent from all the preceeding phases and the fast fluctuating dispensing flow system is decoupled from the slowly flowing, already processed dispensing from tubes connected to the device, said device comprising:

(a) at least one transporting duct having a downstream end and which carries not only the fluid substance towards a duct branching zone, but also contains apparatus for analysis of the particles to be analyzed in the particle sorting mode;

(b) at least one duct branching zone which is delimited approximately by the downstream ends of the transporting ducts and entrances of dispensing duct branching out from this zone;

(c) at least one device causing an increase of the fluid pressure at the entrances of the dispensing ducts through injection of a control medium so that by increasing the pressure at one such duct, the substance, or the particle carried in it, is prevented from entering into this duct but is constrained to enter into such a duct whee, during the same dispensing phase, such a pressure increase does not take place, during the injection, it is the control medium that flows into that duct where this medium is being injected;

(d) at least two dispensing ducts, branching out of the duct branching zone at a small angle relative to each other;

(e) a corresponding buffer cavity at the end of each dispensing duct having a fluid-free volume which is substantially largr than taht of the combined flow system, which consists of all the transporting ducts, the duct branching volume and all the dispensing ducts, so as to compensate for pressure fluctuation and to decouple a rapid fluctuation flow of the fluid in the dispensing ducts from a large amount of the already processed fluid, flowing out of the device slowly and without any appreciable fluctuation;

(f) a two-position multiple-tube valve to direct, at one position, the various processed fluids into other containers, and in the other position, to direct all the fluids into a waste container; and

(g) filtering/collector cavity means connected to the one position of the valve, for selectively increasifng the concentration of the particle suspension after processing so that most of the suspension is separted from the particles.

17. The device according to claim 16, wherein the various substances flow under the effect of pressure applied at the containers of the substances, while the outlets of the device are at atmospheric pressure.

18. The device according to claim 16, wherein the various substances flow under the effect of a vacuum applied at the outlets of the device, while the inlets of the device are at atmospheric pressure.

19. The device according to claim 16, wherein the various substances flow under the effect of pressure applied at the containers of all the substances flowing in the device, while at least some of the outlets of the device are at a vacuum and the remaining outlets are at an arbitrary pressure lower than the pressure applied at the containers.

20. The device according to claim 16, wherein more than one transporting duct is provided, each of the transporting ducts carrying only one type of substance towards the duct branching zone independently from the other transporting ducts.

21. The device according to claim 16, wherein more than one transporting duct is provided, each of the transporting ducts carrying more than one type of fluid substance towards the duct branching zone independently from the other transporting ducts.

22. The device according to claim 16, wherein any of the transporting ducts carry at least one thin stream having a cross-section which is very small relative to that of the duct, the at least one stream being enveloped by at least one kind of fluid subtance as it flows toward the duct branching zone without turbulence.

23. The device according to claim 22, wherein said thin streams have a cross-section geometry that provides a preferred orientation for processing the particles carried by these individual streams.

24. The device according to claim 16, wherein a transporting duct accommodates one of at least one particle analyzing device, and at least parts of such a device for converting an amount of at least one preselected physio-chemical property of the particles into electrical impulses to be processed electronically be connected electronic equipment.

25. The device according to claim 16 or 18, wherein the transporting ducts have a maximal hydrodynamical resistance so as to allow application of as high a vacuum at the outlets of the system as possible.

26. The device according to claim 24, wherein said particle analyzing devices, or parts of them, are only as close to the duct branching zone so that the particles flow-through time between these two places is not shorter than a time which is required to generate the preselected dispensing flow configuration in the duct branching zone.

27. The device according to claim 16, wherein the particle analyzer is an optical system in a epillumination mode and having an object of very short focal length, a transporting duct having a channel form being carved into the top surface of the device and covered by a very thin glass in an airtight manner.

28. The device according to claim 27, wherein the induct branching volume is delimited by the downstream ends of the transporting channels, by the entrance of the dispensing channels, by the bottom surface of this part of the flow system, and by the lower surface of the covering glass, which covers this part of the flow system.

29. The device according to claim 16, wherein some of the ducts are used purely for dispensing purposes.

30. The device according to claim 16, wherein some of the ducts of the system are used only as control ducts, carrying no particles.

31. The device according to claim 16, wherein the control medium is injected into the dispenser duct in such a manner that the amount of the fluid substance to be processed is of a minimum value, that is, the control medium is injected partly into the duct branching volume, which is located immediately in front of the entrance of the duct, and partly into the duct itself.

32. The device according to claim 31, wherein the control medium is injected to an optimum part through an injection duct, having its entrance outside of the device where the injection is carried out by an electromechanical valve.

33. The device according to claim 32, wherein the control medium is a suitable gas whereby the speed of operation of the device is higher relative to a fluidous control medium.

34. The device according to claim 32, wherein the control medium is chosen so that a prescribed mixing occurs between the control medium and the fluid substance to be dispensed and mixed.

35. The device according to claim 32, wherein the fluid substance to be dispensed is a fluid which is not to be diluted during the dispensing process, an inert gas being used as the control medium.

36. The device according to claim 16, and further comprising means for producing a vacuum in front of the entrance of one of the dispenser duct and control duct, the control medium being a gas generated by a suitable physical process.

37. The device according to claim 36, wherein the gas is generated at said optimal place between platinum electrodes through electrolysis.

38. The device according to claim 36, wherein a vapor for the control medium is generated at said optimal place between at least two noncorrosive metals, through electrical arcs or sparks between said metals, by heating up a small volume of the fluid in front of the entrance of the dispenser duct to the boiling temperature.

39. The device according to claim 36, wherein a vapor for the control medium is generated at said optimal place by illuminating the fluid at said place by a sufficiently intense light beam so as to heat said fluid to evaporation.

40. The device according to claim 16, 29 or 30, wherein one of the dispensing and control duct has a minimum length so that the downstream end of the control medium does not reach the downstream end of the duct during the process of injection of the control medium.

41. The device according to claim 16, wherein the buffer cavity is constructed into the body of the device at the downstream end of the corresponding control/dispenser duct, and has a fluid-free volume substantially larger than the whole dispensing flow system, said buffer cavity having an outlet at its bottom of such a size that both the processed fluid and the gaseous control medium flow out of it without disturbing the flow of each other, whereby both the pressure difference and the fluid flucutations are compensated to a very high degree.

42. The device according to claim 16, wherein the buffer cavity is manufactured into the body of the device at the downstream end of the control/dispensing duct carrying the processed particles, said cavity having a fluid-free volume many times larger than that of the whole dispensing flow system, and having an outlet at its bottom of such a size that said outlet does not let the gaseous control medium flow through it where the processed particles leave said cavity but through the effect of buoyancy, said control gas is forced to leave the cavity through a gas outlet hole, situated at the upper part of the cavity, which, being at the same system pressure, compensates the pressure fluctation in this cavity through its large fluid-free volume.

43. The device according to claim 16, and further comprising a body, the filtering/collector cavity being provided in the device body and comprising a cartridge equipped with a bayonet holder;

an inlet tube for the substance entering into this cavity;

a fine membrane filter having openings smaller than the size of the smallest particle to be filtred out of the processed substance;

a rough membrane filter situated under the fine filter;

a rough membrane filter situated under the fine filter;

a vertical capillary system; and

an outlet hole, situated at the upper part of said cylinder, for the stabilization of the pressure difference in the cavity.

44. The device according to claim 16, and further comprising a body, the filtering/collector cavity being provided in the device body and comprising a cartridge containing a bayonet system so that said cartridge is attached, airtight, into said cavity;

an inlet tube provided so as to allow entry of the processed substance into the cavity;

a very fine membrane with openings about no more than 1.0 micron;

a rough membrane filter situated under the very fine membrane filter;

a vertical cylinder having an upper end covered by a metal grid so as to support said other two filters;

an opening at the upper part of said cylinder for the stabilization of the pressure in the cavity; and

a second opening situated at the bottom of the vertical cylinder so as to create a sufficiently large vacuum to suck the suspending fluid and filter out the processed particles.

45. The device according to claim 16, and further comprising a main body, the filtering/collector cavity means being attached to the main device body by flexible tubes and including a bottle having a container part;

an inlet tube situated at the top of the container for the entrance of the suspension into this bottle;

a fine membrane filter having openings not smaller than the size of the smallest particle to be filtered out of the processed suspension;

a rough membrane filter situated under the fine membrane filter;

a vertical capillary sytem; and

an outlet hole situated at the upper part of the bottle, for the stabilization of the pressure difference in the bottle.

46. The device according to claim 16, and further comprising a main body, the filtering/collector cavity means being attached to each particle-carrying suspension outlet of the main device body by flexible tubes and including a bottle having a container part;

an inlet tube situated at the top of said bottle and oriented approximately vertically for the entrance of the processed suspension into said bottle;

a very fine membrane filter having openings of no more than about 1.0 microns in diameter;

a rough membrane filter situated under said very fine filter;

a vertical cylinder having an upper end covered by a metal grid so as to support said two filters at the top of said cylinder;

an opening at the upper part of said bottle for the stabilization of the pressure in the whole flow system; and

a second opening situated at the bottom of said vertical cylinder so as to create a sufficiently large vacuum in the cylinder to suck under the suspension, filtering out of it the processed particles.

47. The device according to any one of claims 43-46, wherein a ring is placed at the time membrane filter so as to delimit the spreading of the filtered particles on said filter.

48. The device according to any one of claims 43-46, wherein a microscope objective holder glass is placed under said inlet tube, said glass being covered by a fine screen so that while the processed particles remain between the openings of said screen, the processed suspension itself flows under to the bottom of the bottle.

49. The device according to claim 16, wherein the vacuum, applied at its outlets, is as high as is permitted by overall construction device.

50. The device according to claim 16, wherein the dispensing ducts branch from the duct branching zone with the smallest possible angle relative to each other, while the control ducts surround these ducts, thereby obtaining an optimum duct branching geometry for the device.

51. The device according to claim 16, wherein the analyzers are of an optical type, the material of the device being a nonfluorescing, black material.

52. The device according to claim 16, wherein the whole device is made of an electrically nonconductive material.

53. The device according to claim 16, wherein several cleaning ducts are formed in the device body, through which the duct branching zone is accessible by one of a jet of cleaning fluid so that extraneous materials are removable from the zone, the cleaning ducts being sealed airtight during operation of the device.

54. The device according to claim 16, wherein the channels, formed in the open surface of the device, are sealed by a very thin, microscope cover glass, using a nonfluorescing and nonreflecting black glue so that undesirable background illumination is substantially reduced.

55. The device according to claim 16, and further comprising a body, all the channels, cavities and ducts being formed in the body of the device in such a manner that any part of the flow system is clearly observable through a stereo microscope so that any extraneous piece therein is easily observable facilitating removal of such pieces form the flow system.

60. A device according to claim 16, wherein the flow of fluid substances includes one of fluids, gases, and aerosols.

61. A device according to claim 16, wherein the fluid control medium is one of a fluid, a gas, and a vapor.

62. A device according to claim 38, wherein the noncorrosive metals are from a group including wolfram, molybdenum and platinum.

63. A device according to claim 39, wherein the light beam is a laser beam.

64. A device according to claim 43, wherein the vertical capillary system is a bundle of glass tubes each having a diameter of approximately 1.0 mm.

65. A device according to claim 45, wherein the vertical capillary system is a bundle of glass tubes, each having a diameter of approximately 1.0 mm, held together by elastic bands.

66. A device according to claim 16, wherein the analyzers are of an optical type, the walls of the ducts and channels being covered by a very thin sheet of a nonfluorescing, black material.

67. A device according to claim 16, wherein various electrical wires incorporated into the device are well insulated from a conductive body of the device.

68. A device according to claim 54, wherein the black glue is an epoxy.

69. A device according to claim 16, wherein the analyzing apparatus is an electronic system which in the dispensing mode of continuous substances has a predetermined format for determining the distribution of the substances.

71. The device according to claim 16, wherein several cleaning ducts are formed in the device body so that the duct branching zone is accessible by a cleaning thread so that extraneous materials are removable from the zone, the cleaning ducts being sealed air-tight during operation of the device.