Instrument for independently and kinetically measuring light transpassion through a plurality of samples

• US 4,936,682 A
• Filed: 08/11/1987
• Issued: 06/26/1990
• Est. Priority Date: 08/11/1987
• Status: Expired due to Term

• Current Assignee: Associates of Cape Cod Incorporated (Seikagaku Corporation)
• Original Assignee: Associates of Cape Cod Incorporated (Seikagaku Corporation)
• Inventors: Hoyt, Joshua K.
• Primary Examiner(s): McGraw, Vincent P.
• Application Number:
  US07/084,098
• Time in Patent Office: 1,050 Days
• Field of Search: 356/440, 356/409, 356/416, 356/414, 356/39, 356/244, 250/576
• US Class Current: 356/414
• CPC Class Codes: G01N 21/253 for batch operation, i.e. multisample apparatus analytical automats G01N35/00
• CPC Class Codes: G01N 2201/0407 with multiple optical units, e.g. one per sample
• CPC Class Codes: G01N 2201/0415 Carrusel, sequential
• CPC Class Codes: G01N 2201/0476 Keyboard controlled, e.g. for plural analysis at one sample, channel selection, coding

Abstract

An analytic instrument for kinetically measuring light absorption characteristics of a plurality of independent samples contained in disposable test tubes arranged in a circular pattern about a single light source. The instrument is designed to be used with a host personal computer and is not specific to any particular type of assay. The instrument obtains and temporarily stores raw data in the form of digitized output signals from the plurality of photodetectors and periodically passes them to the host computer. An incubator has test tube wells arranged in a circular array equidistant from a single incandescent light source. The circular symmetry controls the thermal gradients in the incubator such that all of the samples are disposed on the same isotherm and all of the photodetectors are disposed on the same isotherm. The instrument is designed for use with disposable test tubes, and sources of error arising from optical variation in such test tubes are minimized by providing a separate detector for each tube and by holding the tube fixed with respect to the detector. The light output from the single light source is continuously monitored and is kept constant.

Claims

1. A kinetic analytical instrument having a plurality of independent channels for obtaining output data from a plurality of photodetectors resulting from changes in light transmission through a plurality of samples, said instrument comprising at least a first module, said first module comprising:

a stationary first housing;

first test tube support means substantially fixedly mounted within said first housing and having formed therein a plurality of wells disposed in a substantially circular array and a first plurality of light passages respectively disposed transversely of and intersecting said plurality of wells, each of said plurality of wells being adapted to receive a test tube, said first test tube support means comprising means for respectively fixedly supporting a plurality of test tubes in said plurality of wells and in a substantially circular array wherein said test support means comprises an incubator of a substantially annular shape centered on a center of curvature of said circular array of wells, said incubator including a heater block and further comprising heater means for heating said incubator block, said incubator further including a test tube rack having said wells formed therein and being in the form of a substantially annular block, said heating block having formed therein a receiving opening receiving said test tube rack and wherein said means for fixedly supporting said plurality of test tubes comprises biasing means for biasing test tubes in said wells against interior walls of said wells, said biasing means comprising an elastic ring-like member disposed radially outside said circular array of wells;

a single first measurement constant output light source centrally mounted within said first housing and being so disposed that light radially emitted therefrom passes through each of said first plurality of light passages and traverses each of said plurality of wells, said first measurement light source being disposed substantially equidistant from each of said plurality of wells;

a plurality of measuring photodetectors disposed in said first housing and respectively fixedly mounted with respect to each of said first light passages so as to each receive light from said first measurement light source after said light has traversed said plurality of wells and said plurality of samples and for independently and kinetically measuring light transmission through said plurality of samples while controlling a plurality of variable; and

instrument control means programmed to provide a series of channel select signal.

2. An instrument according to claim 1, said heater block having at least one curved sidewall concentric with said circular array of wells and being disposed therearound.

3. An instrument according to claim 2, further comprising heater power supply means for said heater means and temperature sensing means for sensing a temperature of said heater block, wherein said heater power supply means comprises means responsive to said temperature sensing means for regulating the energy supplied to said heater means so that the temperature of said heater block remains substantially constant.

4. An instrument according to claim 3, wherein said measuring photodetectors are mounted on said incubator in close thermal contact therewith, said heater means and the mass of said incubator collectively comprising means for maintaining each said measuring photodetector at substantially the same constant temperature.

5. An instrument according to claim 1, there being a slot formed in and extending circumferentially of said test tube rack, said ring-like member being received in said slot.

6. An instrument according to claim 1, wherein a plurality of said wells have circular cross-sections have diameters substantially in the range between 0.394 inches and 0.399 inches.

7. An instrument according to claim 1, wherein each of said plurality of wells have circular cross-sections have diameters substantially in the range between 0.470 inches and 0.475 inches.

8. An instrument according to claim 1, wherein said measurement light source is disposed at a center of curvature of said substantially circular array of wells.

9. An instrument according to claim 8, further comprising a control photodetector fixedly mounted so as to receive light from said measuring light source, said power supply means for said measurement light source comprising measurement light regulating means responsive to a signal from said control photodetector for continuously regulating an output of said measurement light power supply so that said signal from said control photodetector remains substantially constant.

10. An instrument according to claim 1, comprising:

first module control means disposed in said first housing and comprising at least;

first power supply means for said first measurement light source;

first well state indicating means associated with a plurality of said first wells for providing an independent indication for each first well indicating whether or not a measurement is to be taken for that well; and

first photodetector interrogating means responsive to said stepping signals for passing an output signal to said instrument control means from each first measuring photodetector that is associated with a first well for which said indication of said first well state indicating means indicates that a measurement is to be taken for that well.

11. An instrument according to claim 10, wherein said control photodetector and said measuring photodetectors are mounted on said test tube support means in close thermal contact therewith, wherein the temperature of said control photodetector will remain substantially equal to the temperatures of said measuring photodetectors.

12. An instrument according to claim 8, further comprising light diffuser means disposed between said measurement light source and said wells.

13. An instrument according to claim 8, further comprising spectrally-selective light filter means disposed between said measurement light source and said wells.

14. An instrument according to claim 10, said well state indicating means comprising a plurality of electromechanical switches respectively associated with a corresponding plurality of said wells, each said switch having actuator means extending into its corresponding well and disposed for being contacted by a test tube present within said corresponding well, wherein the introduction of a test tube into a well causes a change of state of the switch that is associated therewith, said photodetector interrogating means being independently responsive to the state of each said switch.

15. An instrument according to claim 1, wherein said programmed instrument control means includes at least a central processing unit and memory means, said instrument further comprising digitizing means for digitizing said signals from said measuring photodetectors, said instrument control means being programmed to store said digitized signals in said memory means.

16. An instrument according to claim 15, said instrument control means further comprising interface means for communicating said instrument control means with a host computer.

17. An instrument according to claim 10 further comprising at least a second module, said second module comprising:

a second housing;

second test tube support means substantially fixedly mounted within said second housing and having formed therein an additional plurality of wells disposed in a substantially circular array and a second plurality of light passages respectively disposed transversely of and intersecting said additional wells, each of said additional wells being adapted to receive a test tube, said second test tube support means comprising means for supporting a plurality of test tubes at fixed locations and in a second substantially circular array;

a single second measurement light source mounted within said second housing and being so disposed that light therefrom will pass through each said second light passage and will traverse each of said additional wells, said second measurement light source being disposed substantially equidistant from each of said additional wells;

a second plurality of measuring photodetectors disposed in said second housing and respectively fixedly mounted with respect to said second light passages so as to receive light from said second measurement light source after said light has traversed said additional wells; and

second module control means disposed in said second housing and comprising at least;

second power supply means for said second measurement light source;

additional well state indicating means associated with a plurality of said additional wells for providing an independent indication for each of said second wells indicating whether or not a measurement is to be taken for that well; and

second photodetector interrogating means responsive to said stepping signals for sequentially and repeatingly passing an output signal to said instrument control means from each second measuring photodetector that is associated with one of said additional wells for which said indication of said additional well state indicating means indicates that a measurement is to be taken for that well.

18. An instrument according to claim 17, wherein said instrument control means is disposed in one of said first and second modules and is in communication with said first and second module control means, said instrument control means including at least a central processing unit and memory means, said instrument further comprising digitizing means for digitizing said signals from said measuring photodetectors, said instrument control means being programmed to access said first and second module control means and to store said digitized signals in said memory means.

19. An instrument according to claim 18, further comprising interface means for communicating said instrument control means with a host computer.