Multiplex sequencing

• US 4,942,124 A
• Filed: 08/04/1988
• Issued: 07/17/1990
• Est. Priority Date: 08/11/1987
• Status: Expired due to Term

• Current Assignee: Harvard University
• Original Assignee: President and Fellows of Harvard College (Harvard Corporation)
• Inventors: Church, George M.
• Primary Examiner(s): YARBROUGH, AMELIA
• Application Number:
  US07/228,596
• Time in Patent Office: 712 Days
• Field of Search: 435/6, 435/803, 435/172.3, 435/320, 536/27, 436/501, 935/77, 935/78, 935/23, 935/24, 935/29
• US Class Current: 435/6.12
• CPC Class Codes: C12Q 1/6813 Hybridisation assays
• CPC Class Codes: C12Q 1/6869 Methods for sequencing
• CPC Class Codes: C12Q 1/6874 involving nucleic acid arrays, e.g. sequencing by hybridisation
• CPC Class Codes: C12Q 2521/501 Ligase
• CPC Class Codes: C12Q 2563/179 the label being a nucleic acid
• CPC Class Codes: Y10S 435/803 Physical recovery methods, e.g. chromatography, grinding

Abstract

This invention features vectors and a method for sequencing DNA. The method includes the steps of:

(a) ligating the DNA into a vector comprising a tag sequence, the tag sequence includes at least 15 bases, wherein the tag sequence will not hybridize to the DNA under stringent hybridization conditions and is unique in the vector, to form a hybrid vector,

(b) treating the hybrid vector in a plurality of vessels to produce fragments comprising the tag sequence, wherein the fragments differ in length and terminate at a fixed known base or bases, wherein the fixed known base or bases differs in each vessel,

(c) separating the fragments from each vessel according to their size,

(d) hybridizing the fragments with an oligonucleotide able to hybridize specifically with the tag sequence, and

(e) detecting the pattern of hybridization of the tag sequence, wherein the pattern reflects the nucleotide sequence of the DNA.

Claims

1. A method for sequencing a DNA specimen comprising:

(a) providing a set of at least two vectors, each vector of said set comprising a cloning site and including at least one tag sequence at a position within 50 bases of said cloning site, each tag sequence in each vector differing from each tag sequence of every other vector of said set, and each being incapable of hybridizing to said DNA specimen under stringent conditions,

(b) providing a first and a second DNA sequence from said DNA specimen, said first DNA sequence being different from said second DNA sequence,

(c) ligating said first DNA sequence into the cloning site of one of said vectors, and said second DNA sequence into the cloning side of a second of said vectors, thereby producing a plurality of hybrid vectors,

(d) providing a pool of said hybrid vectors,

(e) treating separate aliquots of said pool in a plurality of vessels to produce fragments comprising said tag sequence, wherein said fragments in each vessel differ in length from each other and all terminate at a fixed known base or bases, wherein said fixed known base or bases in one of said vessels differ from said fixed known base or bases in another of said vessels,

(f) separating said fragments comprising tag sequences from each said vessel according to their size,

(g) hybridizing the separated fragments of step (e) under stringent conditions with a first oligonucleotide probe able to hybridize specifically with one of said tag sequences, and

(h) detecting the pattern of hybridization wherein said pattern reflects the nucleotide sequence of said DNA specimen.

2. The method of claim 1 wherein the pattern detected in step (h) reflects the nucleotide sequence of said first DNA sequence and said method further comprises the steps of rehybridizing the separate fragments of step (f) under stringent conditions with a second oligonucleotide probe different from the first and detecting a second pattern of rehybridization wherein said second pattern of hybridization reflects the nucleotide sequence of said second DNA sequence.

3. The method of claim 1 or 2 in which each said vector comprises two said tag sequences.

4. The method of claim 3 in which said two tag sequences on the same vector are different from each other.

5. The method of claim 1 or 2 further comprising binding the fragments of step (e) to a solid support prior to said hybridizing.

6. The method of claim 1 or 2 wherein said stringent hybridization conditions comprise conditions in which mismatched duplexes are melted to form single stranded molecules.

7. A method for simultaneously sequencing multiple DNA molecules comprising,

(a) providing a pool of individually different DNA tag sequences, each tag sequence in said pool being foreign to, and incapable of hybridizing under stringent conditions to, any of said DNA molecules to be sequenced;

(b) from separate aliquots of said pool of tag sequences, generating a plurality of sets of hybrid DNA fragments in separate vessels, said hybrid fragments in each vessel differing in length from one another and terminating in a fixed known base or bases, wherein said fixed known base or bases in one of said vessels differ from said fixed known base or bases in another of said vessels;

(i) each of said fragments comprising at least one of said tag sequences and at least part of one and only one of said DNA molecules to be sequenced, each of said fragments that includes part of any given one said DNA molecules to be sequenced having a tag sequence that is different from each of the tag sequences in fragments that include part of any other one of said DNA molecules to be sequenced,

(ii) each fragment of a first set of said hybrid DNA fragments comprising a first one of said tags, said first set of fragments comprising fragments of different lengths of a first one of said DNA molecules to be sequenced; and

each fragment of a second set of said sets fragments comprising a second one of said tags, said second set of fragments comprising fragments of different lengths of a second one of said DNA molecules to be sequenced,

(c) separating said first and said second set of fragments according to their size, yielding size-separated fragments,

(d) hybridizing said size=separated fragments under stringent conditions with a first oligonucleotide probe able to hybridize specifically with said first one of said tag sequences,

(e) detecting a first pattern of hybridization with said first probe, wherein said first pattern reflects the nucleotide sequence of at least a part of said first DNA molecule to be sequenced,

(f) hybridizing said size separated fragments under stringent conditions with a second oligonucleotide probe able to hybridize specifically with said second one of said tag sequences, and

(g) detecting a second pattern of hybridization with said second probe, wherein said second pattern reflects the nucleotide sequence of said second DNA molecule to be sequenced.

8. The method of claim 7 in which each said hybrid DNA fragment comprises two said tag sequences.

9. The method of claim 8 in which said two tag sequences are different from each other.

10. The method of claim 8 or 9 further comprising binding the fragments of step (e) to a solid support prior to said hybridizing.

11. The method of claim 8 or 9 wherein said stringent hybridization condition comprises conditions in which mismatched duplexes are melted to from single stranded molecules.

12. The method of claim 7 in which said pool of DNA tag sequences is provided by providing a set of at least two vectors, each vector of said set comprising a cloning site and including at least one of said tag sequences at a position within 50 bases of said cloning site, each tag sequence in each vector differing from each tag sequence of every other vector of said set, ligating said first one of said DNA molecules to be sequenced into the cloning site of one of said vectors, and ligating said second one of said DNA molecules to be sequenced into the cloning site of a second of said vectors, and then providing a pool of the resulting hybrid vectors comprising said tag sequences.