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Electrochemical methods of assay

  • US 4,945,045 A
  • Filed: 06/23/1988
  • Issued: 07/31/1990
  • Est. Priority Date: 07/06/1984
  • Status: Expired due to Term
First Claim
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1. In a homogeneous method of assaying a ligand in a sample using electrochemical apparatus containing a working electrode and components comprising:

  • (a) the sample, and(b) a specific binding partner to the ligand, or a specific binding partner to the ligand and at least one further reagent selected from ligand analogues and specific binding partners to the ligand, the improvement which comprises said components including(c) an electron-source or electron-acceptor selected from oxidoreductase enzymes in cooperation with a substrate therefor and non-enzyme electron sources;

    one of components (b) being labelled with an electron-transfer mediator capable of aiding the transfer of electrons between the electron-source or electron-acceptor and the working electrode by accepting electrons from the electron-source and donating them to the working electrode, or accepting electrons from said electrode and donating them to the electron-acceptor; and

    which method includes the step of determining whether the said transfer of electrons is perturbed by formation of a complex of said ligand with a specific binding partner.

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