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Evanescent wave background fluorescence/absorbance detection

  • US 4,945,245 A
  • Filed: 07/14/1988
  • Issued: 07/31/1990
  • Est. Priority Date: 01/14/1986
  • Status: Expired due to Fees
First Claim
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1. A method for the optical analysis of a slurry, which slurry comprises:

  • (a) a continuous phase which comprises a component that fluoresces at an optically detectable wavelength, and(b) a discontinuous phase which comprises a component that also fluoresces at said optically detectable wavelength,the method comprising the steps of;

    (i) optically exciting said slurry with an evanescent wave having a wavelength that excites the fluorescence of said components and simultaneously(ii) detecting the intensity of the fluorescence resulting therefrom, and then(iii) relating said detected intensity of fluorescence to the concentration of said component in said continuous phase, such that said detected intensity of fluorescence is independent of the concentration of the component in said discontinuous phase that also fluoresces.

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