Process for development of acceptance of transplanted organs and tissues

• US 4,946,438 A
• Filed: 06/12/1989
• Issued: 08/07/1990
• Est. Priority Date: 09/01/1983
• Status: Expired due to Fees

• Current Assignee: Trustees Of Columbia University In The City Of New York (Columbia University)
• Original Assignee: Trustees Of Columbia University In The City Of New York (Columbia University)
• Inventors: Lau, Henry T., Hardy, Mark A., Reemtsma, Keith
• Primary Examiner(s): Richman, Barry S.
• Assistant Examiner(s): Wallen, T. J.
• Application Number:
  US07/365,013
• Time in Patent Office: 421 Days
• Field of Search: 435/1, 435/2, 435/173, 435/283, 424/95, 424/101, 128/395, 604/4, 604/5, 604/6, 604/20, 604/49, 604/52, 604/905, 604/53, 204/157.15, 204/158.21
• US Class Current: 604/500
• CPC Class Codes: A61K 35/12 Materials from mammals; Compositions comprising non-specified tissues or cells; Compositions comprising non-embryonic stem cells; Genetically modified cells vaccines or medicinal preparations containing antigens or antibodies A61K39/00
• CPC Class Codes: A61K 35/14 Blood; Artificial blood perfluorocarbons A61K31/02; umbilical cord blood A61K35/51; haemoglobin A61K38/42
• CPC Class Codes: A61K 41/0023 Agression treatment or altering
• CPC Class Codes: A61L 27/3616 Blood, e.g. platelet-rich plasma
• CPC Class Codes: A61L 27/3691 characterised by physical conditions of the treatment, e.g. applying a compressive force to the composition, pressure cycles, ultrasonic/sonication or microwave treatment, lyophilisation

Abstract

A patient'"'"'s acceptance of transplanted organs and tissues may be enhanced by treating donor specific blood with a suitable dose of ultraviolet radiation for an appropriate period of time, transfusing the irradiated blood into the patient during a suitable pretransplantation time period and then transplanting the organ or tissue into the subject. Acceptance of transplanted organs and tissues can also be enhanced by treating the organs or tissues to be transplanted with a suitable dose of ultraviolet radiation for an appropriate period of time and then transplanting the organ or tissue. Suitably irradiated donor specific blood, organs and tissues are preferred for use in transplantation.

Claims

1. A method for transplanting a foreign organ or tissue from a donor into a subject so as to enhance acceptance of the transplanted foreign organ or tissue by the subject which comprises:

(a) irradiating donor-specific blood with ultraviolet-B radiation at a dosage of less than 1000 J/m² so as to render the blood capable of inducing donor-specific immunological unresponsiveness in the subject;

(b) transfusing the irradiated, donor-specific blood into the subject prior to transplanting the foreign organ or tissue into the subject so as to enhance subsequent acceptance of the organ or tissue by the subject; and

(c) thereafter transplanting the foreign organ or tissue into the subject.

2. A method according to claim 1, wherein the subject is a human being.

3. A method according to claim 1, wherein the foreign organ or tissue is or is derived from kidney, heart, lung, liver, intestine, or bone marrow.

4. A method according to claim 1, wherein the irradiation is effected for a period of time greater than about ten minutes.

5. A method according to claim 1, wherein the transfusion is performed more than about one week prior to the transplantation and comprises more than one transfusion.

6. A method according to claim 5, wherein the transfusion comprises transfusions at three, at two, and at one week prior to transplantation.