Apparatus and method for dilution and mixing of liquid samples

• US 4,946,795 A
• Filed: 08/18/1989
• Issued: 08/07/1990
• Est. Priority Date: 08/27/1987
• Status: Expired due to Term

• Current Assignee: Roche Diagnostics Operations Incorporated (Roche Holding AG)
• Original Assignee: Biotrack, Inc. (Novartis Ag)
• Inventors: Gibbons, Ian, Allen, Jimmy D., Robertson, Channing R., Hillman, Robert S.
• Primary Examiner(s): Richman, Barry S.
• Assistant Examiner(s): Wallen, T. J.
• Application Number:
  US07/395,808
• Time in Patent Office: 354 Days
• Field of Search: 436/179, 436/176, 436/180, 422/99, 422/100, 422/296-299, 422/102, 73/864.02, 73/864.12, 73/864.52, 141/4, 141/7, 141/9, 141/285
• US Class Current: 436/179
• CPC Class Codes: B01F 33/30 Micromixers
• CPC Class Codes: B01F 33/452 using independent floating stirring elements
• CPC Class Codes: B01F 35/80 Forming a predetermined ratio of the substances to be mixed controlling ratio of two or more flows of fluid or fluent material G05D11/02
• CPC Class Codes: G01N 1/38 Diluting, dispersing or mixing samples
• CPC Class Codes: G01N 33/04 Dairy products
• CPC Class Codes: Y10T 436/25625 Dilution
• CPC Class Codes: Y10T 436/2575 Volumetric liquid transfer

Abstract

An apparatus for diluting a sample with a diluent, comprising a fixed volume measuring chamber, a fixed volume receiving chamber and fluid receiving relationship to the measuring chamber, a gas vent in the receiving chamber, a stop flow junction between the measuring chamber and the receiving chamber, a sample application site in fluid donating relationship to the measuring chamber, wherein the vertical height difference between the sample application site and the stop flow junction is insufficient to provide a hydrostatic pressure capable of overcoming backpressure at the stop flow junction when sample is applied to the sample application site, and the diluent application site and fluid donating relationship to the measuring chamber. Sample and diluent are added sequentially to the apparatus of the invention without requiring any intervening operations. Various means are provided for restarting flow, which causes diluent to wash the sample into the receiving chamber, where the two can be mixed.

Claims

1. An apparatus for automatically diluting a sample with a diluent, comprising:

housing means containing

sample application site means for receiving a sample;

measuring chamber means having a first volume;

receiving chamber means having a second volume, wherein said second volume is greater than said first volume;

diluent application site means for receiving a diluent;

first flow means for delivering a sample from said sample application site means to said measuring chamber means by the sum of capillary and gravitational forces upon addition of sample to said sample application site means;

second flow means for delivering diluent from said diluent application site means to said measuring chamber means by the sum of capillary and gravitational forces upon addition of diluent to said diluent application site means;

third flow means for delivering sample and diluent from said measuring chamber means to said receiving chamber means by the sum of capillary and gravitational forces;

a stop flow junction located in said third flow means and adapted to the surface tension characteristics of the sample so as to provide sufficient back pressure resulting from contact between said sample and wall means of said housing means to prevent sample from flowing through said stop flow junction when sample is present at said stop flow junction;

whereby addition of diluent to said diluent application site means after sample is added to said application site means and initiation of flow at said stop flow junction cause sample in said measuring chamber means to be delivered by said capillary and gravitational forces to said receiving chamber means along with a portion of said diluent, whereby a fixed ratio of sample to diluent is present in said receiving chamber means.

2. The apparatus of claim 1, further comprising means for starting flow at said stop flow junction.

3. The apparatus of claim 2, wherein said means for starting flow is a moveable part of said apparatus capable of being actuated to contact sample present at said stop flow junction.

4. The apparatus of claim 3, wherein said moveable part is a magnetic stir bar in said receiving chamber.

5. The apparatus of claim 2, wherein said means for starting flow comprises a stir bar in said receiving chamber and a roughened surface or projection in said receiving chamber, whereby motion of said stir bar in said receiving chamber causes vibrations in said apparatus that start flow at said stop flow junction.

6. The apparatus of claim 1, wherein said measuring chamber means has a volume from 1 μ

L to 30 μ

L.

7. The apparatus of claim 1, wherein said receiving chamber means has a volume of from 3 μ

L to 1,000 μ

L.

8. The apparatus of claim 1, wherein said measuring chamber means is a capillary chamber.

9. A method of diluting a sample with a diluent, comprising:

adding an unmeasured volume of said sample to sample application site means of a device in which said sample flows by the sum of capillary and gravitational forces to measuring chamber means having a first volume, said measuring chamber means being terminated by a stop flow junction which stops sample flow as a result of back pressure resulting from interaction of said sample with wall means of said device at said stop flow junction, said device further comprising receiving chamber means having a second volume greater than said first volume, said receiving chamber means being located in fluid receiving relationship to said measuring chamber means;

then adding said diluent to diluent application site means of said device, wherein said diluent flows by the sum of capillary and gravitational forces to said measuring chamber means; and

then starting flow at said stop flow junction, wherein said starting flow comprises;

moving said device, whereby said moving overcomes said back pressure and allows sample and diluent to flow through said stop flow junction into said receiving chamber means,

whereby a fixed ratio of sample and diluent fills said receiving chamber means.

10. The method of claim 9, wherein said motion is caused by a force applied externally to said apparatus.

11. The method of claim 9, wherein said motion is caused by contact between a magnetic stirring bar in said apparatus and a surface in said apparatus.

12. A method of diluting a sample with a diluent, comprising:

adding an unmeasured volume of said sample to sample application site means of a device in which said sample flows by the sum of capillary and gravitational forces to measuring chamber means having a first volume, said measuring chamber means being terminated by a stop flow junction which stops sample flow as a result of back pressure resulting from interaction of said sample with wall means of said device at said stop flow junction, said device further comprising receiving chamber means having a second volume greater than said first volume, said receiving chamber means being located in fluid receiving relationship to said measuring chamber means;

then adding said diluent to diluent application site means of said device, wherein said diluent flows by the sum of capillary and gravitational forces to said measuring chamber means; and

then starting flow at said stop flow junction, wherein said starting flow comprises contacting liquid present at said stop flow junction with a moveable part of said apparatus, wherein sample and diluent flow through said stop flow junction into said receiving chamber means, whereby a fixed ratio of sample and diluent fills said receiving chamber means.

13. The method of claim 12, wherein starting flow is caused by contact between said moveable part and said sample at said stop flow junction.

14. The method of claim 13, wherein said moveable part is a magnetically activated stir bar in said receiving chamber.

15. The method of claim 14, wherein said method further comprises mixing said sample and said diluent in said receiving chamber means with said stir bar.