Chemical sensor

• US 4,948,727 A
• Filed: 10/11/1985
• Issued: 08/14/1990
• Est. Priority Date: 10/12/1984
• Status: Expired due to Term

• Current Assignee: Medisense Incorporated (Abbott Laboratories Incorporated)
• Original Assignee: Medisense Incorporated (Abbott Laboratories Incorporated)
• Inventors: Bramwell, Helena, Cass, Anthony E. G.
• Primary Examiner(s): Nucker, Christine M.
• Assistant Examiner(s): Beisner, William H.
• Application Number:
  US06/786,974
• Time in Patent Office: 1,768 Days
• Field of Search: 435/18, 435/288, 435/291, 435/817, 204/403, 204/1 E
• US Class Current: 205/777.5
• CPC Class Codes: C12Q 1/005 involving specific analytes or enzymes including groups of enzymes, e.g. oxydases; C12Q1/004 takes precedence
• CPC Class Codes: G01N 33/9486 Analgesics, e.g. opiates, aspirine
• CPC Class Codes: Y10S 435/817 Enzyme or microbe electrode

Abstract

The present invention is concerned with a chemical sensor, more specifically with a chemical sensor which is capable of detecting the presence of, measuring a quantity of or monitoring the level of N-acetyl primary aromatic amines, such as paracetamol, in whole blood.

The specification relates to a method of assay of the type in which an electrode poised at a suitable potential is contacted with a system comprising;

(a) a sample suspected to contain paracetamol or a derivative thereof, and

(b) an enzyme capable of catalyzing the hydrolysis of an N-acylated primary aromatic amine or a derivative thereof, and,

wherein the current flowing in the electrode is a measure of the quantity of hydrolysis products formed and thereby of the concentration of N-acylated primary aromatic amine or derivatives thereof in the sample.

Claims

1. A method of assay of a liquid sample of a bodily fluid to determine presence or content of N-acylated primary aromatic amine, comprising:

(a) contacting said sample with an enzyme capable of catalysing the hydrolysis of N-acylated primary aromatic amine to form an enzyme/amine system comprising said enzyme and said N-acylated primary amine;

(b) contacting said enzyme/amine system with an electrode;

(c) poising said electrode at a potential responsive to direct non-mediated transfer of charge from the system to the electrode, and

(d) detecting or measuring the current flow as an indication of presence of amount of N-acylated primary aromatic amine in the system.

2. A method as claimed in claim 1 in which said system is a liquid mixture of paracetmol and an enzyme capable of the hydrolysis thereof, and said electrode is contacted with said liquid system whereby the direction or measurement of the paracetamol is effected.

3. The method of claim 1 in which said potential is less than 200 mV.

4. A method as claimed in claim 1 in which the liquid samples contains paracetamol as the primary aromatic amine and in which said enzyme is located at least at the surface of said electrode.

5. A method as claimed in claim 3 in which the enzyme is the aryl acylamidase, EC 3.5.1.13.

6. A sensor electrode comprising:

(a) a substrate material;

(b) an enzyme capable of catalysing the hydrolysis of paracetamol, said enzyme being located at least on the surface of said substrate; and

, (c) means to maintain said electrode at a poised potential, said electrode being free from electron-transfer mediator compounds, whereby charge is directly transferred to the electrode material, without presence of a mediator compound, when the electrode at said potential is contacted with a liquid sample containing paracetamol.

7. The sensor electrode of claim 6 comprising means to maintain said electrode at a poised potential less than 200 mV.

8. A sensor electrode as claimed in claim 6 comprising a membrane at the electrode surface in which said enzyme is immobilised.

9. A sensor electrode as claimed in any one of claims 6 or 8 in which the electrode comprises electrically conductive material chosen from gold, platinum, nickel and carbon associated with said substrate.

10. A sensor electrode as claimed in claim 6 or 9 in which the said enzyme is the aryl acrylamidase, EC 3.5.1.13.