Quantitative luminescence imaging system

• US 4,948,975 A
• Filed: 09/08/1988
• Issued: 08/14/1990
• Est. Priority Date: 09/08/1988
• Status: Expired due to Term

• Current Assignee: Alice Venables, United States Of America As Represented By The Secretary Of The Air Force
• Original Assignee: United States Of America As Represented By The Secretary Of The Air Force
• Inventors: Stahl, Kurt A., Batishko, Charles R., Kiel, Johnathan L., Erwin, David N.
• Primary Examiner(s): FIELDS, CAROLYN
• Application Number:
  US07/241,992
• Time in Patent Office: 705 Days
• Field of Search: 250/361 C, 250/361 R, 250/458.1, 250/459.1, 250/461.1, 250/461.2, 250/484.1 A, 250/337, 436/172, 422/52
• US Class Current: 250/361.00C
• CPC Class Codes: G01N 21/76 Chemiluminescence; Bioluminescence

Abstract

The QLIS images and quantifies low-level chemiluminescent reactions in an electromagnetic field. It is capable of real time nonperturbing measurement and simultaneous recording of many biochemical and chemical reactions such as luminescent immunoassays or enzyme assays. The system comprises image transfer optics, a low-light level digitizing camera with image intensifying microchannel plates, an image process or, and a control computer. The image transfer optics may be a fiber image guide with a bend, or a microscope, to take the light outside of the RF field. Output of the camera is transformed into a localized rate of cumulative digitalized data or enhanced video display or hard-copy images. The system may be used as a luminescent microdosimetry device for radiofrequency or microwave radiation, as a thermal dosimeter, or in the dosimetry of ultra-sound (sonoluminescence) or ionizing radiation. It provides a near-real-time system capable of measuring the extremely low light levels from luminescent reactions in electromagnetic fields in the areas of chemiluminescence assays and thermal microdosimetry, and is capable of near-real-time imaging of the sample to allow spatial distribution analysis of the reaction. It can be used to instrument three distinctly different irradiation configurations, comprising (1) RF waveguide irradiation of a small Petri-dish-shaped sample cell, (2) RF irradiation of samples in a microscope for the microscopie imaging and measurement, and (3) RF irradiation of small to human body-sized samples in an anechoic chamber.

Claims

1. A quantitative luminescence imaging system (QLIS) comprising a sample excitation unit which includes excitation means for exciting a sample by irradiation in an RF field to produce a luminescent reaction, video camera means, image transfer optic means arranged for coupling light from said sample to an input of the video camera means, the light being converted in the video camera means to electronic signals at an output thereof, and an electronic data processing system coupled to the output of the video camera means for processing the signals, the image transfer optic means being arranged so that the input of the video camera means is outside of the environment of said excitation means.

2. A quantitative luminescence imaging system according to claim 1,

which is a macroscopic system, wherein the sample excitation unit comprises an RF waveguide having a sample chamber for said sample, the video camera is a low light level camera, and the data processing system comprises an image processor and a control computer.

3. A quantitative luminescence imaging system according to claim 2.

wherein the image transfer optics is a bundle of optic fibers having a bend;

and

wherein the video camera means comprises a microchannel plate image intensifier optically coupled to a video camera tube, with the image intensifier comprising a fiber optic faceplate for the light input, a photocathode, focusing electrodes, microchannel plates, and a phosphor screen for the light output.

4. A quantitative luminescence imaging system according to claim 1,

which is a microscopic system, wherein the sample excitation unit comprises an RF antenna/sample holder at the end of an RF transmission line, the excitation means being an RF field at the RF antenna/sample holder when an RF signal is supplied via the transmission line, the image transfer optics is a microscope having the sample holder located at the normal specimen point in the light path of the microscope, and the video camera means is a low light level camera attached to the microscope.

5. A quantitative luminescence imaging system (QLIS) comprising a sample excitation unit which includes excitation means for exciting a sample by microwave radiation as an activator to produce a luminescent reaction, video camera means, image transfer optic means arranged for coupling light from said sample to an input of the video camera means, the light being converted in the video camera means to electronic signals at an output thereof, and an electronic data processing system coupled to the output of the video camera means for processing the signals, the image transfer optic means being arranged so that the input of the video camera means is outside of the environment of said excitation means.