Quantitative luminescence imaging system
First Claim
1. A quantitative luminescence imaging system (QLIS) comprising a sample excitation unit which includes excitation means for exciting a sample by irradiation in an RF field to produce a luminescent reaction, video camera means, image transfer optic means arranged for coupling light from said sample to an input of the video camera means, the light being converted in the video camera means to electronic signals at an output thereof, and an electronic data processing system coupled to the output of the video camera means for processing the signals, the image transfer optic means being arranged so that the input of the video camera means is outside of the environment of said excitation means.
3 Assignments
0 Petitions
Accused Products
Abstract
The QLIS images and quantifies low-level chemiluminescent reactions in an electromagnetic field. It is capable of real time nonperturbing measurement and simultaneous recording of many biochemical and chemical reactions such as luminescent immunoassays or enzyme assays. The system comprises image transfer optics, a low-light level digitizing camera with image intensifying microchannel plates, an image process or, and a control computer. The image transfer optics may be a fiber image guide with a bend, or a microscope, to take the light outside of the RF field. Output of the camera is transformed into a localized rate of cumulative digitalized data or enhanced video display or hard-copy images. The system may be used as a luminescent microdosimetry device for radiofrequency or microwave radiation, as a thermal dosimeter, or in the dosimetry of ultra-sound (sonoluminescence) or ionizing radiation. It provides a near-real-time system capable of measuring the extremely low light levels from luminescent reactions in electromagnetic fields in the areas of chemiluminescence assays and thermal microdosimetry, and is capable of near-real-time imaging of the sample to allow spatial distribution analysis of the reaction. It can be used to instrument three distinctly different irradiation configurations, comprising (1) RF waveguide irradiation of a small Petri-dish-shaped sample cell, (2) RF irradiation of samples in a microscope for the microscopie imaging and measurement, and (3) RF irradiation of small to human body-sized samples in an anechoic chamber.
-
Citations
5 Claims
- 1. A quantitative luminescence imaging system (QLIS) comprising a sample excitation unit which includes excitation means for exciting a sample by irradiation in an RF field to produce a luminescent reaction, video camera means, image transfer optic means arranged for coupling light from said sample to an input of the video camera means, the light being converted in the video camera means to electronic signals at an output thereof, and an electronic data processing system coupled to the output of the video camera means for processing the signals, the image transfer optic means being arranged so that the input of the video camera means is outside of the environment of said excitation means.
-
5. A quantitative luminescence imaging system (QLIS) comprising a sample excitation unit which includes excitation means for exciting a sample by microwave radiation as an activator to produce a luminescent reaction, video camera means, image transfer optic means arranged for coupling light from said sample to an input of the video camera means, the light being converted in the video camera means to electronic signals at an output thereof, and an electronic data processing system coupled to the output of the video camera means for processing the signals, the image transfer optic means being arranged so that the input of the video camera means is outside of the environment of said excitation means.
Specification