Prolonged enhanced chemiluminescence
First Claim
1. In a chemiluminescence process comprising the contacting of a chemiluminescence precursor, an oxidant, an enzyme, and a chemiluminescence enhancer, the improvement comprising conducting the process in the presence of a nitrogen compound selected from the group consisting of ammonia, an aromatic heterocyclic compound and a water-soluble organic amine.
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Abstract
A chemiluminescence process comprising the contacting of a chemiluminescence precursor, an oxidant, an enzyme, a chemiluminescence enhancer and a nitrogen compound selected from the group consisting of ammonia and water-soluble organic amines. The reaction of such process can be used in detection of nucleic acid hybrids, antibodies, antigens and peroxidase enzymes and in producing light.
50 Citations
19 Claims
- 1. In a chemiluminescence process comprising the contacting of a chemiluminescence precursor, an oxidant, an enzyme, and a chemiluminescence enhancer, the improvement comprising conducting the process in the presence of a nitrogen compound selected from the group consisting of ammonia, an aromatic heterocyclic compound and a water-soluble organic amine.
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16. A chemiluminescence assay for the determination of a peroxidase enzyme comprising contacting an unknown sample with a chemiluminescence precursor, an oxidant, a chemiluminescence enhancer and a nitrogen compound selected from the group consisting of ammonia, an aromatic heterocyclic compound and a water-soluble organic amine, determining the extent of light emission and correlating the determination of light emission to the determination of the enzyme.
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17. A test kit for conducting a chemiluminescence assay comprising in containers a chemiluminescence precursor, an enzyme, an oxidant, a chemiluminescence enhancer and a nitrogen compound selected from the group consisting of ammonia, an aromatic heterocyclic compound and a water-soluble organic amine.
Specification