Fermentation of trichoderma reesei and apparatus therefor

• US 4,952,505 A
• Filed: 08/08/1988
• Issued: 08/28/1990
• Est. Priority Date: 08/08/1988
• Status: Expired due to Term

• Current Assignee: Florida State University (State University System of Florida)
• Original Assignee: Florida State University (State University System of Florida)
• Inventors: Cho, Michael Y.
• Primary Examiner(s): Wax, Robert A.
• Application Number:
  US07/229,823
• Time in Patent Office: 750 Days
• Field of Search: 435/209, 435/945
• US Class Current: 435/209
• CPC Class Codes: C12N 1/145 Fungal isolates
• CPC Class Codes: C12N 9/2437 Cellulases (3.2.1.4; 3.2.1.74; 3.2.1.91; 3.2.1.150)
• CPC Class Codes: C12R 2001/885 Trichoderma
• CPC Class Codes: C12Y 302/01004 Cellulase (3.2.1.4), i.e. endo-1,4-beta-glucanase
• CPC Class Codes: Y10S 435/945 Trichoderma

Abstract

A process for producing enzyme cellulases by the fermentation of Trichoderma reesei in an aqueous nutrient medium containing assimilable sources of cellulose, nitrogen, phosphate, magnesium and iron in the presence of an oxygen containing atmosphere and a fermentation apparatus for the aerobic fermentation of microorganisms in a liquid medium at a pressure in excess of about 7 atmospheres. The method comprises fermenting the Trichoderma reesei at a temperature of between about 26° C. and 31° C. while maintaining the oxygen containing atmosphere at a pressure of about 1 atmosphere until the Trichoderma reesei enter the late stationary growth phase, thereafter, gradually and steadily increasing the pressure of the oxygen containing atmosphere until it is in excess of about 7 atmospheres and culturing the Trichoderma reesei at said increased pressure and at a temperature of between about 40° C. and about 60° C., thereby resulting in the production of enzyme cellulases by the Trichoderma reesei, and recovering the enzyme cellulases.

Claims

1. A process for producing enzyme cellulases by the fermentation of Trichoderma reesei (ATCC 26921) in an aqueous nutrient medium containing assimilable sources of cellulose, nitrogen, phosphate magnesium and iron in the presence of an oxygen containing atmosphere, the method comprising

fermenting the Trichoderma reesei at a temperature of between about 26°

C. and 31°

C. while maintaining the oxygen containing atmosphere at a pressure of about 1 atmosphere until the Trichoderma reesei enter the late stationary growth phase,

thereafter, gradually and steadily increasing the pressure of the oxygen containing atmosphere until it is in excess of about 7 atmospheres and fermenting the Trichoderma reesei at said increased pressure and at an increased temperature of between about 40°

C. and about 60°

C., thereby resulting in the production of enzyme cellulases by the Trichoderma reesei, and

recovering the enzyme cellulases.

2. A process as set forth in claim 1 wherein said aqueous nutrient medium comprises a source of calcium.

3. A process as set forth in claim 1 wherein said aqueous nutrient medium comprises trace amounts of manganese, cobalt, zinc and molybdenum.

4. A process as set forth in claim 1 wherein said increased pressure is between about 117 and about 127 atmospheres.

5. A process as set forth in claim 1 wherein said increased temperature is about 45°

C.

6. A process as set forth in claim 4 wherein said increased pressure is about 122 atmospheres.