×

Enzymatically-cleavable chemiluminescent fused polycyclic ring-containing 1,2-dioxetanes

  • US 4,952,707 A
  • Filed: 06/30/1988
  • Issued: 08/28/1990
  • Est. Priority Date: 06/30/1988
  • Status: Expired due to Term
First Claim
Patent Images

1. An enzymatically cleavable chemiluminescent 1,2-dioxetane compound having the formula:

  • ##STR9## wherein R1 is hydrogen, or a bond when R2 is a substituent bound to the dioxetane ring through a spiro linkage, or an organic substituent that does not interfere with the production of light but satisfies the valence of the dioxetane ring carbon atom to which it is attached;

    R2 is a fused polycyclic ring-containing fluorophore moiety bound to the dioxetane ring through a single bond or a spiro linkage and having an enzymatically cleavable labile ring substituent containing a bond which, when cleaved, renders the polycyclic moiety electron-rich to in turn render the dioxetane compound decompasable to emit light, the enzymatically cleavable labile ring substituent'"'"'s point of attachment to the fused polycyclic moiety, in relation to the fused polycyclic moiety'"'"'s point of attachment to the dioxetane ring, being such that the total number of ring atoms separating these points of attachment, including the ring atoms at these points of attachment, is an odd whole number; and

    T is a stabilizing group that prevents the dioxetane compound from decomposing before the enzymatically cleavable labile ring substituent'"'"'s bond is cleaved.

View all claims
  • 9 Assignments
Timeline View
Assignment View
    ×
    ×