Thin film membrane enzyme reactor and method of using same

• US 4,956,289 A
• Filed: 03/16/1987
• Issued: 09/11/1990
• Est. Priority Date: 03/16/1987
• Status: Expired due to Fees

• Current Assignee: Brunswick Corporation One Brunswick Plaza Skokie IL 60070
• Original Assignee: Brunswick Corporation
• Inventors: Wrasidlo, Wolfgang J., Hofmann, Frieder K.
• Primary Examiner(s): Hill, Jr., Robert J.
• Application Number:
  US07/026,380
• Time in Patent Office: 1,275 Days
• Field of Search: 435/176-182, 435/240, 435/241, 435/243, 435/817, 210/632, 210/651, 210/456, 210/489, 210/488, 210/490
• US Class Current: 435/180
• CPC Class Codes: C12M 21/18 Apparatus specially designed for the use of free, immobilized or carrier-bound enzymes
• CPC Class Codes: C12M 25/14 Scaffolds; Matrices in general C12N5/0068
• CPC Class Codes: C12M 29/04 Filters; Permeable or porous membranes or plates, e.g. dialysis
• CPC Class Codes: Y10S 435/817 Enzyme or microbe electrode

Abstract

An enzyme reactor system is provided based on the entrapment of an enzyme in a hydrogel layer coated on a support, and confined by an ultraporous thin film membrane diffusion barrier. The diffusion barrier confines the enzyme, but lets substrate and reaction products diffuse feeely into and out of the hydrogel layer. In an alternate embodiment, the support is formed of an ultraporous thin film membrane diffusion barrier on a microporous or macroporous support, through which the reaction products can diffuse freely, but through which neither enzyme nor substrate can pass. In this embodiment, the product is recovered in high purity, free of substrate and enzyme.

Claims

1. An enzyme reactor consisting essentially of:

a support;

an enzyme hydrogel layer physically entrapped on said support; and

a porous thin film diffusion membrane on said enzyme hydrogel layer, wherein said thin film diffusion membrane has a thickness of from about 0.0012 to 0.2 microns and a pore size sufficient to permit diffusion of a substrate for said enzyme and of the product of a reaction of said substrate catalyzed by said enzyme, but which will not permit said enzyme to pass through said thin film diffusion membrane, said thin film diffusion membrane adapted to direct exposure of a process stream.

2. The reactor of claim 1, wherein said support is impermeable.

3. The reactor of claim 1, wherein said support comprises:

a support surface thin film layer in contact with said enzyme which is impermeable, and

an open porous layer physically supporting said support surface thin film layer.

4. The reactor of claim 1, wherein said support comprises:

a support surface thin film layer in contact with said enzyme which is porous to the product of a reaction of a substrate and said enzyme, but which will not permit said enzyme to pass through said support surface layer, and

an open porous layer physically supporting said support surface thin film layer.

5. The reactor of claim 4, wherein there is interposed between said support surface thin film layer and said open porous layer physically supporting said support surface thin film layer a thin, freely porous gel layer.

6. The reactor of claim 1, wherein said support comprises:

a support surface thin film layer in contact with said enzyme which is porous to the product of a reaction of a substrate and said enzyme, but which will not permit said substrate or said enzyme to pass through said support surface layer, and an open porous layer physically supporting said support surface thin film layer.

7. The reactor of claim 6, wherein there is interposed between said support surface thin film layer and said open porous layer physically supporting said support surface thin film layer a thin, freely porous gel layer.

8. The reactor of claim 1, wherein said support is a dense support having a high surface area per unit volume.

9. The reactor of claim 1, wherein said enzyme hydrogel comprises an aqueous gel of an enzyme and a hydrogel forming polymer.

10. The reactor of claim 9, wherein said hydrogel forming polymer is cross linked.

11. The reactor of claim 9, wherein said hydrogel forming polymer is irreversibly bound to said support and to said thin film membrane.

12. The reactor of claim 9, wherein said enzyme is physically entrapped in said hydrogel forming polymer in an amount of from about 10 to about 90 weight percent of said hydrogel forming polymer, on a solids basis.

13. A method for using an enzyme reactor the steps consisting essentially of:

forming an enzyme reactor which comprises;

a support;

an enzyme hydrogel layer physically entrapped on said support; and

a porous thin film diffusion membrane on said enzyme hydrogel layer, wherein said thin film diffusion membrane has a thickness of from about 0.0012 to 0.2 microns and a pore size sufficient to permit diffusion of a substrate for said enzyme and of the product of a reaction of said substrate catalyzed by said enzyme, but which will not permit said enzyme to pass through said thin film diffusion membrane;

placing said enzyme reactor into a reactor vessel;

dissolving substrate for said enzyme in a solvent therefor;

passing said dissolved substrate through said reactor vessel in such a manner that said substrate and said solvent therefor are in direct exposure to said porous thin film diffusion membrane;

allowing said substrate to interact with said enzyme, thereby forming a product;

collecting the product of said interaction of said substrate and said enzyme.

14. The method of claim 13, wherein said substrate diffuses through said thin film diffusion membrane and into said enzyme hydrogel layer prior to interacting with said enzyme.

15. The method of claim 13, wherein said product is collected by allowing said product to diffuse from said enzyme hydrogel layer through said thin film diffusion membrane and into said solvent for said substrate.

16. The method of claim 13, wherein said support further comprises

a support surface thin film layer in contact with said enzyme which is porous to the product of a reaction of a substrate and said enzyme, but which will not permit said subsrate or said enzyme to pass through said support surface layer, and

an open porous layer physically supporting said support surface thin film layer.

17. The method of claim 16, wherein said substrate diffuses through said thin film diffusion membrane and into said enzyme hydrogel layer prior to interacting with said enzyme.

18. The method of claim 16, wherein there is a solvent for said product in contact with the surface of said open porous layer of said support which is not in contact with said support surface thin film layer.

19. The method of claim 18, wherein said product diffuses through said support surface thin film layer and said open porous layer of said support into said solvent for said product.

20. The method of claim 18, wherein said said substrate is soluble in said solvent for said product.

21. The method of claim 18, wherein said substrate is insoluble in said solvent for said product.

22. The method of claim 18, wherein said product is insoluble in said solvent for said substrate.

23. The method of claim 16, wherein there is interposed between said support surface thin film layer and said open porous layer physically supporting said support surface thin film layer a thin, freely porous gel layer.

24. The method of claim 23, wherein there is a solvent for said product in contact with the surface of said open porous layer of said support which is not in contact with said support surface thin film layer.

25. The method of claim 23, wherein said product diffuses through said support surface thin film layer and said open porous layer of said support into said solvent for said product.

26. The method of claim 23, wherein said said substrate is soluble in said solvent for said product.

27. The method of claim 23, wherein said substrate is insoluble in said solvent for said product.

28. The method of claim 23, wherein said product is insoluble in said solvent for said substrate.