Bovine fibroblast growth factor

• US 4,956,455 A
• Filed: 06/20/1985
• Issued: 09/11/1990
• Est. Priority Date: 03/05/1984
• Status: Expired due to Term

• Current Assignee: Salk Institute for Biological Studies
• Original Assignee: Salk Institute for Biological Studies
• Inventors: Esch, Frederick S., Baird, Andrew, Bohlen, Peter, Gospodarowicz, Denis, Ling, Nicholas C.
• Primary Examiner(s): Draper, Garnette D.
• Application Number:
  US06/747,154
• Time in Patent Office: 1,909 Days
• Field of Search: 260/112.5 R, 260/112 R, 530/399, 530/820, 530/839, 530/827, 435/69.4
• US Class Current: 530/399
• CPC Class Codes: A61K 38/00 Medicinal preparations containing peptides peptides containing beta-lactam rings A61K31/00; cyclic dipeptides not having in their molecule any other peptide link than those which form their ring, e.g. piperazine-2,5-diones, A61K31/00; ergot alkaloids of the cyclic peptide type A61K31/48; containing macromolecular compounds having statistically distributed amino acid units A61K31/74; medicinal preparations containing antigens or antibodies A61K39/00; medicinal preparations characterised by the non-active ingredients, e.g. peptides as drug carriers, A61K47/00
• CPC Class Codes: C07K 14/503 basic FGF [bFGF]
• CPC Class Codes: Y10S 530/82 Proteins from microorganisms
• CPC Class Codes: Y10S 530/827 Proteins from mammals or birds
• CPC Class Codes: Y10S 530/839 Nerves; brain

Abstract

Substantially pure bovine pituitary fibroblast growth factor, a 146 amino acid residue polypeptide, is produced. The amino acid residue sequence of bpFGF is disclosed as well as a DNA chain encoding the polypeptide. By appropriately inserting a synthesized DNA chain into a cloning vector and using the cloning vector to transform cells, synthetic bpFGF can be obtained from transformed cell lines, both prokaryotic and eukaryotic.

Claims

1. Substantially pure basic fibroblast growth factor containing the amino acid sequence:

##STR3##

2. Basic fibroblast growth factor which is substantially pure, which is capable of stimulating proliferation of endothelial cells, and which has a MW of about 16,250, being a polypeptide containing about 140 amino acids.

3. Basic fibroblast growth factor according to claim 2 wherein the N-terminus has the sequence Pro-Ala-Leu-Pro-Glu-Asp-Gly-Gly-Ser.

4. Basic fibroblast growth factor according to claim 3 isolated from bovine pituitaries.

5. Basic fibroblast growth factor according to claim 2 isolated from bovine brain tissue.

6. Basic fibroblast growth factor according to claim 3 isolated from bovine pituitaries wherein the polypeptide has about the following amino acid composition:

Asx(12), Thr(4), Ser(9), Glx(14), Pro(9) Gly(16),Ala(9), Cys(4), Val(6), Met(2), Ile(3), Leu(13),Tyr(7),Phe(7),His(2), Trp(1),Lys(14), and Arg(12).

7. Basic fibroblast growth factor according to claim 2, which is capable of stimulating proliferation of endothelial cells in in vitro culture to at least about 5 times normal level in about 4 days.