Detection of Reticulocytes, RNA and DNA
First Claim
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1. In a process for detecting reticulocytes, RNA or DNA in a sample, the improvement comprising:
- adding to said sample a fluorescent dye having the formula ##STR7## wherein;
X=0, S, Se or C (CH3)2 ;
R1 =alkyl having from 1-6 carbons;
R2 =alkyl having from 1-6 carbons;
R3 =fused benzene, alkyl (having 1-6 carbons), methoxy or hydrogen;
R4 =alkyl having 1-6 carbons, methoxy or hydrogen; and
n=zero or an integer from 1-6;
exciting said sample with light of excitation wavelength; and
measuring fluorescence emitted from said sample.
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Abstract
Reticulocytes, RNA or DNA are stained with a dye for detection in a flow cytometer. The dye has the formula: ##STR1## Wherein X=O, S, se or C(CH3)2 ;
R1 =alkyl having from 1-6 carbons;
R2 =alkyl having from 1-6 carbons;
R3 =fused benzene, alkyl (having 1-6 carbons), methoxy or is absent;
R4 =alkyl having 1-6 carbons, methoxy or is absent; and
n=zero or an integer from 1-6.
98 Citations
10 Claims
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1. In a process for detecting reticulocytes, RNA or DNA in a sample, the improvement comprising:
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adding to said sample a fluorescent dye having the formula ##STR7## wherein;
X=0, S, Se or C (CH3)2 ;R1 =alkyl having from 1-6 carbons; R2 =alkyl having from 1-6 carbons; R3 =fused benzene, alkyl (having 1-6 carbons), methoxy or hydrogen; R4 =alkyl having 1-6 carbons, methoxy or hydrogen; and n=zero or an integer from 1-6; exciting said sample with light of excitation wavelength; and
measuring fluorescence emitted from said sample. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10)
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Current AssigneeBecton, Dickinson & Co
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Original AssigneeBecton, Dickinson & Co
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InventorsLee, Linda G., Chen, Chia-Huei
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Primary Examiner(s)Sneed, H. M. S.
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Assistant Examiner(s)Saba, J.
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Application NumberUS07/332,657Time in Patent Office533 DaysField of Search436/63, 436/94, 436/172, 250/461.2US Class Current436/63CPC Class CodesG01N 33/80 involving blood groups or b...Y10T 436/143333 Saccharide [e.g., DNA, etc.]
