Immunoseparating strip
First Claim
1. A method for determining the presence of an analyte, that is capable of binding specifically to an antibody, in a sample suspected of containing said analyte, which comprises:
- (a) contacting, with a test solution containing said sample, antibodies to said analyte and a conjugate of said analyte and a label, a contact portion of a strip of bibulous material capable of being traversed by said test solution by capillary migration, said strip containing non-diffusively bound to a situs on said strip separated from said contact portion a first receptor capable of binding to said conjugate, the surface area of said situs being less than that of said strip, said strip further containing a second receptor capable of binding said antibodies to said analyte, said second receptor being non-diffusively bound to said strip between said situs and said contact portion,(b) allowing at least a portion of said test solution to traverse said strip by capillary migration and thereby contact said situs,(c) exposing said strip to a signal producing means capable of interacting with said label to produce a signal in relation to the amount of analyte in the test solution, and(d) detecting said signal at said situs and relating said signal to the presence of said analyte.
2 Assignments
0 Petitions
Accused Products
Abstract
A method and device for determining the presence of an analyte in a sample suspected of containing the analyte are disclosed. The method involves contacting a test solution containing the sample, an antibody for the analyte, and a conjugate of the analyte and a label with a contact portion of a strip of bibulous material capable of being traversed by the test solution through capillary action. The strip contains a first receptor capable of binding to the conjugate. The first receptor is non-diffusively bound to a situs on the strip separate from the contact portion of the strip. The strip further contains a second receptor capable of binding the antibody to the analyte between the situs and the contact portion. The second receptor is non-diffusively bound to the strip. At least a portion of the test solution is allowed to traverse the strip by capillary action and thereby contact the situs. The strip is exposed to a signal producing means capable of interacting with the label to produce a signal in relation to the amount of analyte in the test solution. The signal produced at the situs is then detected.
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Citations
29 Claims
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1. A method for determining the presence of an analyte, that is capable of binding specifically to an antibody, in a sample suspected of containing said analyte, which comprises:
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(a) contacting, with a test solution containing said sample, antibodies to said analyte and a conjugate of said analyte and a label, a contact portion of a strip of bibulous material capable of being traversed by said test solution by capillary migration, said strip containing non-diffusively bound to a situs on said strip separated from said contact portion a first receptor capable of binding to said conjugate, the surface area of said situs being less than that of said strip, said strip further containing a second receptor capable of binding said antibodies to said analyte, said second receptor being non-diffusively bound to said strip between said situs and said contact portion, (b) allowing at least a portion of said test solution to traverse said strip by capillary migration and thereby contact said situs, (c) exposing said strip to a signal producing means capable of interacting with said label to produce a signal in relation to the amount of analyte in the test solution, and (d) detecting said signal at said situs and relating said signal to the presence of said analyte. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15)
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16. A method for determining the presence of one or more of a plurality of analytes, each of which is capable of binding specifically to an antibody, in a sample suspected of containing said analytes, which comprises:
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(a) contacting, with a test solution containing said sample, a plurality of antibodies each corresponding to one of said analytes and a plurality of conjugates, each comprising one of said analytes and a label, a contact portion of a strip of bibulous material capable of being traversed by said test solution by capillary migration, said strip containing one or more situses on said strip separate from said contact portion, each of said situses having non-diffusively bound thereto a first receptor capable of binding to one of said conjugates, the surface area of each of said situses being less than that of said strip, said strip further containing a second receptor capable of binding said plurality of antibodies to said analyte, said second receptor being non-diffusively bound to said strip between said situses and said contact portion, (b) allowing at least a portion of said test solution to traverse said strip by capillary migration and thereby contact said situses, (c) exposing said strip to a signal producing means capable of interacting with said label(s) to produce a signal at each situs in relation to the amount of each analyte in the test solution, and (d) detecting said signal at each of said situses and relating the signal at each of said situses to the presence of one or more analytes. - View Dependent Claims (17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29)
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Specification