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Immunoseparating strip

  • US 4,959,307 A
  • Filed: 09/05/1986
  • Issued: 09/25/1990
  • Est. Priority Date: 09/05/1986
  • Status: Expired due to Term
First Claim
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1. A method for determining the presence of an analyte, that is capable of binding specifically to an antibody, in a sample suspected of containing said analyte, which comprises:

  • (a) contacting, with a test solution containing said sample, antibodies to said analyte and a conjugate of said analyte and a label, a contact portion of a strip of bibulous material capable of being traversed by said test solution by capillary migration, said strip containing non-diffusively bound to a situs on said strip separated from said contact portion a first receptor capable of binding to said conjugate, the surface area of said situs being less than that of said strip, said strip further containing a second receptor capable of binding said antibodies to said analyte, said second receptor being non-diffusively bound to said strip between said situs and said contact portion,(b) allowing at least a portion of said test solution to traverse said strip by capillary migration and thereby contact said situs,(c) exposing said strip to a signal producing means capable of interacting with said label to produce a signal in relation to the amount of analyte in the test solution, and(d) detecting said signal at said situs and relating said signal to the presence of said analyte.

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