Desoxyribonucleic acid; microorganisms; diagnostic agent for delta infection and the use thereof

• US 4,959,462 A
• Filed: 01/28/1986
• Issued: 09/25/1990
• Est. Priority Date: 07/31/1984
• Status: Expired due to Term

• Current Assignee: Nederlandse Centrale Organisatie Voor Toegepast-Natuurwetenschappelijk Onderzoek
• Original Assignee: Nederlandse Centrale Organisatie Voor Toegepast-Natuurwetenschappelijk Onderzoek
• Inventors: Dijkema, Reinder, Kos, Antonides
• Primary Examiner(s): Yarbrough, Amelia B.
• Assistant Examiner(s): MARSCHEL, ARDIN H
• Application Number:
  US06/823,196
• Time in Patent Office: 1,701 Days
• Field of Search: 536/27, 435/320, 435/6, 435/7, 435/172.3, 436/501, 436/811, 436/813, 935/3, 935/29, 935/56, 935/78
• US Class Current: 536/24.32
• CPC Class Codes: C07K 14/005 from viruses
• CPC Class Codes: C12N 2730/10122 New viral proteins or individual genes, new structural or functional aspects of known viral proteins or genes
• CPC Class Codes: C12Q 1/705 for herpetoviridae, e.g. herpes simplex, varicella zoster
• CPC Class Codes: C12Q 1/706 for hepatitis
• CPC Class Codes: G01N 33/5765 Hepatitis delta antigen
• CPC Class Codes: Y10S 436/811 Test for named disease, body condition or organ function
• CPC Class Codes: Y10S 436/813 Cancer

Abstract

This invention relates to DNA having at least 60% homology relative to γ-RNA or a portion thereof and the capacity of selective hybridization with δ-RNA. Such DNA, if provided with a detectable label, such as ³² P can be used as a diagnostic agent to diagnose δ infection.

Claims

1. The DNA having at least 60% homology relative to a portion of δ

-RNA and being capable of selective hybridization with δ

-RNA.

2. The DNA as claimed in claim 1, comprising copy DNA of a portion of the δ

-RNA, said copy DNA being capable of selective hybridization with δ

-RNA.

3. A diagnostic agent for diagnosing δ

infection, comprising DNA as claimed in any one of claims 1 and 2, provided with a detectable label.

4. A diagnostic agent as claimed in claim 3, in which the DNA is provided with the radio label ³² p.

5. Recombinant DNA containing vector DNA and an insertion therein of DNA of claim 1, having at least 60% homology relative to a portion of δ

-RNA and being capable of selective hybridization with δ

-RNA.

6. Recombinant DNA as claimed in claim 5, containing copy DNA of a portion of the δ

-RNA, said copy DNA being capable of selective hybridization with δ

-RNA.

8. The DNA as claimed in claim 2, selected from the group consisting of DNA having the following base sequence

7. A DNA selected from the group consisting of DNA having the following base sequence

9. Recombinant DNA selected from the group consisting of DNA having the following base sequence