Supercritical fluid chromatography

• US 4,962,662 A
• Filed: 07/10/1989
• Issued: 10/16/1990
• Est. Priority Date: 02/17/1988
• Status: Expired due to Fees

• Current Assignee: Hewlett-Packard Company (HP Inc.)
• Original Assignee: Hewlett-Packard Company (HP Inc.)
• Inventors: Berger, Terry A.
• Primary Examiner(s): Williams, Hezron E.
• Assistant Examiner(s): Roskos, Joseph W.
• Application Number:
  US07/377,420
• Time in Patent Office: 463 Days
• Field of Search: 73/23.1, 73/61.1 C, 210/198.2
• US Class Current: 73/23.42
• CPC Class Codes: B01D 15/40 using supercritical fluid as mobile phase or eluent
• CPC Class Codes: G01N 2030/3038 temperature control of column exit, e.g. of restrictors
• CPC Class Codes: G01N 2030/3076 using specially adapted T(t) profile
• CPC Class Codes: G01N 2030/324 speed, flow rate
• CPC Class Codes: G01N 30/02 Column chromatography
• CPC Class Codes: G01N 30/28 Control of physical parameters of the fluid carrier
• CPC Class Codes: G01N 30/30 of temperature

Abstract

In a system for analyzing a chemical sample by chromatographic separation of said sample into components a method and apparatus are provided for maximizing detector resolution and minimizing duration by maintaining the linear velocity of the mobile phase at a desired level by affecting the viscosity and density characteristics of the mobile phase as it passes through a restrictor by programming the restrictor temperature.

Claims

1. An apparatus for controlling the mass flow of a mobile phase in a chromatographic analysis system, said apparatus comprising:

a restrictor, through which the mobile phase passes, and heater means, which transfers heat to said restrictor, for heating said mobile phase in said restrictor so that for a given mobile phase pressure the mass flow of said mobile phase is maintained at a preselected level by modifying the temperature of said mobile phase in said restrictor.

2. The apparatus of claim 1, wherein said mass flow is maintained at a preselected constant level.

3. The apparatus of claim 1, wherein said chromatographic analysis system comprises a column through which said mobile phase passes and wherein said restrictor is connected to said column so that the mass flow of said mobile phase in said column is controlled.

4. The apparatus of claim 3, wherein said chromatographic analysis system further comprises a splitter having an analyzed port and a non-analyzed port, said column being connected to said analyzed port, said apparatus further comprising a second restrictor connected to said non-analyzed port and second heater means, which transfers heat to said second restrictor, for heating said mobile phase in said second restrictor so that the mass flow of said mobile phase in said splitter and said column is controlled.

5. The apparatus of claim 1, wherein said chromatographic analysis system comprises a splitter having an output port, said mobile phase passing through said splitter, and wherein said restrictor is connected to said output port so that the mass flow of said mobile phase in said splitter is controlled.

6. An apparatus for controlling the linear velocity of a pressurized mobile phase in a chromatographic analysis system comprising:

a restrictor through which the mobile phase passes, and mass flow control means for controlling the mass flow of said mobile phase passing through said restrictor, so that the linear velocity of said mobile phase is controlled by controlling the mass flow of said mobile phase.

7. The apparatus of claim 6, wherein said mass flow control means comprises, heater means, which transfers heat to said restrictor, for heating said mobile phase in said restrictor so that for a given mobile phase pressure the mass flow of said mobile phase is maintained at a preselected level by modifying the temperature of said mobile phase in said restrictor.

8. A method for controlling the mass flow of a pressurized mobile phase in a chromatographic analysis system comprising the steps of:

passing said mobile phase through restrictor, and heating said mobile phase in said restrictor so that for a given mobile phase pressure the mass flow of said mobile phase is maintained at a preselected level by modifying the temperature of said mobile phase in said restrictor.

9. A method for controlling the linear velocity of a pressurized mobile phase in a chromatographic analysis system comprising the steps of:

passing the mobile phase through a restrictor, and controlling the mass flow of said mobile phase passing through said restrictor, so that the linear velocity of said mobile phase is controlled by controlling the mass flow of said mobile phase.

10. The method of claim 9, wherein the step of controlling the mass flow of said mobile phase passing through said restrictor comprises the step of heating said mobile phase in said restrictor so that for a given mobile phase pressure the mass flow of said mobile phase is maintained at a preselected level by modifying the temperature of said mobile phase in said restrictor.