Method of identity analyte-binding peptides

• US 4,963,263 A
• Filed: 05/16/1989
• Issued: 10/16/1990
• Est. Priority Date: 03/24/1988
• Status: Expired due to Term

• Current Assignee: Telik, Inc. (MabVax Therapeutics Holdings, Inc.)
• Original Assignee: Terrapin Technologies, Inc.
• Inventors: Kauvar, Lawrence M.
• Primary Examiner(s): Therkorn, Ernest G.
• Application Number:
  US07/355,042
• Time in Patent Office: 518 Days
• Field of Search: 210/635, 210/656, 210/658, 210/198.2, 210/502.1, 502/400, 502/401, 502/402, 502/403, 502/404, 530/413, 436/161, 422/70
• US Class Current: 210/635
• CPC Class Codes: B01D 15/3804 Affinity chromatography
• CPC Class Codes: B01D 15/3809 of the antigen-antibody type, e.g. protein A, G, L chromatography
• CPC Class Codes: B01D 15/422 Displacement mode
• CPC Class Codes: B01J 20/3204 Inorganic carriers, supports or substrates
• CPC Class Codes: B01J 20/3212 consisting of a polymer obtained by reactions otherwise than involving only carbon to carbon unsaturated bonds
• CPC Class Codes: B01J 20/3274 Proteins, nucleic acids, polysaccharides, antibodies or antigens
• CPC Class Codes: C07K 1/22 Affinity chromatography or related techniques based upon selective absorption processes
• CPC Class Codes: G01N 30/00 Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation G01N3/00, G01N5/00, G01N7/00, G01N9/00, G01N11/00, G01N13/00, G01N15/00, G01N17/00, G01N19/00, G01N21/00, G01N22/00, G01N23/00, G01N24/00, G01N25/00, G01N27/00, G01N29/00 take precedence
• CPC Class Codes: G01N 30/02 Column chromatography

Abstract

A method for affinity chromatography or adsorption of a designated analyte utilizes a paralog as the affinity partner. The immobilized paralog can be used in purification or analysis of the analyte; the paralog can also be used as a substitute for antibody in an immunoassay. The paralog is identified by screening candidate peptide sequences of 4-20 amino acids for specific affinity to the analyte.

Claims

1. A method to identify a peptide having a sequence of 4-20 amino acids useful for the conduct of affinity chromatography with respect to an analyte,

wherein said peptide has specific affinity for said analyte, which method comprises:

screening, for ability to bind said analyte, a panel of individual candidate peptides wherein said candidate peptides have systematically varied values of at least two parameters selected from the group consisting of hydrophobic index, amphipathic characteristics, and charge pattern.

2. The method of claim 1 wherein the candidate peptides have sequences of 5-15 amino acids.

3. The method of claim 1 wherein said screening step is conducted by assessing the ability of analyte to compete with a labeled peptide mixture, which mixture is capable of binding to all candidate peptides of the panel, for binding to each candidate peptide in the panel.

4. The method of claim 3 wherein said peptide mixtures are labeled by iodinating acetylation.

5. The method of claim 1 wherein said two parameters are hydrophobicity and amphipatic characteristics.

6. The method of claim 1 wherein said two parameters are hydrophobicity and charge pattern.

7. The method of claim 1 wherein said two parameters are amphiphatic characteristics and charge pattern.