DNA detection method
First Claim
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1. A conjugate comprising a high affinity single-stranded DNA binding protein (BP) covalently linked to a detectable label (dl), wherein said conjugate is capable of binding with high affinity via said BP directly to DNA to be detected.
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Abstract
Picogram amounts of DNA can be detected in a sample by the use of high-affinity, single-stranded DNA binding proteins. The assay is applicable not only to pure DNA samples but also to samples containing significant amounts of protein.
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6 Claims
- 1. A conjugate comprising a high affinity single-stranded DNA binding protein (BP) covalently linked to a detectable label (dl), wherein said conjugate is capable of binding with high affinity via said BP directly to DNA to be detected.
- 5. A conjugate comprising a high affinity single-stranded DNA binding protein (BP) covalently linked to a specific binding pair member (sbpm), wherein said conjugate is capable of binding with high affinity via said BP directly to DNA to be detected.
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