Genetic identification employing DNA probes of variable number tandem repeat loci

• US 4,963,663 A
• Filed: 02/08/1989
• Issued: 10/16/1990
• Est. Priority Date: 12/23/1988
• Status: Expired due to Term

• Current Assignee: University of Utah Research Foundation (State of Utah)
• Original Assignee: University of Utah (State of Utah)
• Inventors: Nakamura, Yusuke, Leppert, Mark F., O'Connell, Peter, White, Raymond L.
• Primary Examiner(s): Wax, Robert A.
• Assistant Examiner(s): Marschel, Ardin H.
• Application Number:
  US07/307,820
• Time in Patent Office: 615 Days
• Field of Search: 435/6, 436/63, 536/27, 935/10, 935/19, 935/29, 935/73, 935/78
• US Class Current: 536/24.31
• CPC Class Codes: C12Q 1/683 involving restriction enzymes, e.g. restriction fragment length polymorphism [RFLP]
• CPC Class Codes: C12Q 1/6876 Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
• CPC Class Codes: C12Q 2600/156 Polymorphic or mutational markers

Abstract

The present invention is related to the identification of cloned DNA sequences that reveal individual multiallele loci. The loci are used in the process of the present invention to provide convenient and accurate genetic identification. A large number of clones that recognize VNTR loci have been isolated from a cosmid library and characterized.

Claims

1. A nucleic acid fragment selected from the group consisting of pYNH24, the VNTR-containing fragment of pYNH24, a nucleic acid fragment having substantial sequence homology to said VNTR-containing fragment and a nucleic acid fragment which is capable of hybridizing to the single locus specified by pYNH24.

2. A nucleic acid fragment selected from the group consisting of pCMM6, the VNTR-containing fragment of pCMM6, a nucleic acid fragment having substantial sequence homology to said VNTR-containing fragment and a nucleic acid fragment which is capable of hybridizing to the single locus specified by pCMM6.

3. A nucleic acid fragment selected from the group consisting of pCMM66, the VNTR-containing fragment of pCMM66, a nucleic acid fragment having substantial sequence homology to said VNTR-containing containing fragment and a nucleic acid fragment which is capable of hybridizing to the single locus specified by pCMM66.

4. A nucleic acid fragment selected from the group consisting of cCMM77, the VNTR-containing fragment of cCMM77, a nucleic acid fragment having substantial sequence homology to said VNTR-containing fragment and a nucleic acid fragment which is capable of hybridizing to the single locus specified by cCMM77.

5. A nucleic acid fragment selected from the group consisting of pCMM86, the VNTR-containing fragment of pCMM86, a nucleic acid fragment having substantial sequence homology to said VNTR-containing fragment and a nucleic acid fragment which is capable of hybridizing to the single locus specified by pCMM86.

6. A nucleic acid fragment selected from the group consisting of pMLJ14, the VNTR-containing fragment of pMLJ14, a nucleic acid fragment having substantial sequence homology to said VNTR-containing fragment and a nucleic acid fragment which is capable of hybridizing to the single locus specified by pMLJ14.

7. A nucleic acid fragment selected from the group consisting of cMLJ205, the VNTR-containing fragment of cMLJ205, a nucleic acid fragment having substantial sequence homology to said VNTR-containing fragment and a nucleic acid fragment which is capable of hybridizing to the single locus specified by cMLJ205.

8. A nucleic acid fragment selected from the group consisting of pYNZ22, the VNTR-containing fragment of pYNZ22, a nucleic acid fragment having substantial sequence homology to said VNTR-containing fragment and a nucleic acid fragment which is capable of hybridizing to the single locus specified by pYNZ22.

9. A nucleic acid fragment selected from the group consisting of pJCZ3.1, the VNTR-containing fragment of pJCZ3.1, a nucleic acid fragment having substantial sequence homology to said VNTR-containing fragment and a nucleic acid fragment which is capable of hybridizing to the single locus specified by pJCZ3.1.

10. A nucleic acid fragment selected from the group consisting of pJCZ16.2, the VNTR-containing fragment of pJCZ16.2, a nucleic acid fragment having substantial sequence homology to said VNTR-containing fragment and a nucleic acid fragment which is capable of hybridizing to the single locus specified by pJCZ16.2.

11. A nucleic acid fragment selected from the group consisting of pJCZ67, the VNTR-containing fragment of pJCZ67, a nucleic acid fragment having substantial sequence homology to said VNTR-containing fragment and a nucleic acid fragment which is capable of hybridizing to the single locus specified by pJCZ67.

12. A nucleic acid fragment selected from the group consisting of pYNZ21, the VNTR-containing fragment of pYNZ21, a nucleic acid fragment having substantial sequence homology to said VNTR-containing fragment and a nucleic acid fragment which is capable of hybridizing to the single locus specified by pYNZ21.

13. A nucleic acid fragment selected from the group consisting of pCMM101, the VNTR-containing fragment of pCMM101, a nucleic acid fragment having substantial sequence homology to said VNTR-containing fragment and a nucleic acid fragment which is capable of hybridizing to the single locus specified by pCMM101.

14. A nucleic acid fragment selected from the group consisting of cEFD64, the VNTR-containing fragment of cEFD64, a nucleic acid fragment having substantial sequence homology to said VNTR-containing fragment and a nucleic acid fragment which is capable of hybridizing to the single locus specified by cEFD64.

15. A nucleic acid fragment selected from the group consisting of cEFD52, the VNTR-containing fragment of cEFD52, a nucleic acid fragment having substantial sequence homology to said VNTR-containing fragment and a nucleic acid fragment which is capable of hybridizing to the single locus specified by cEFD52.

16. A nucleic acid fragment selected from the group consisting of pEFD139, the VNTR-containing fragment of pEFD139, a nucleic acid fragment having substantial sequence homology to said VNTR-containing fragment and a nucleic acid fragment which is capable of hybridizing to the single locus specified by pEFD139.

17. A nucleic acid fragment selected from the group consisting of pEFD126.3, the VNTR-containing fragment of pEFD126.3, a nucleic acid fragment having substantial sequence homology to said VNTR-containing fragment and a nucleic acid fragment which is capable of hybridizing to the single locus specified by pEFD126.3.

18. A nucleic acid fragment selected from the group consisting of pTHH33, the VNTR-containing fragment of pTHH33, a nucleic acid fragment having substantial sequence homology to said VNTR-containing fragment and a nucleic acid fragment which is capable of hybridizing to the single locus specified by pTHH33.

19. A nucleic acid fragment selected from the group consisting of pTHH59, the VNTR-containing fragment of pTHH59, a nucleic acid fragment having substantial sequence homology to said VNTR-containing fragment and a nucleic acid fragment which is capable of hybridizing to the single locus specified by pTHH59.

20. A nucleic acid fragment selected from the group consisting of cMHZ47, the VNTR-containing fragment of cMHZ47, a nucleic acid fragment having substantial sequence homology to said VNTR-containing fragment and a nucleic acid fragment which is capable of hybridizing to the single locus specified by cMHZ47.

21. A nucleic acid fragment selected from the group consisting of pMHZ10, the VNTR-containing fragment of pMHZ10, a nucleic acid fragment having substantial sequence homology to said VNTR-containing fragment and a nucleic acid fragment which is capable of hybridizing to the single locus specified by pMHZ10.

22. A nucleic acid fragment selected from the group consisting of pMHZ13, the VNTR-containing fragment of pMHZ13, a nucleic acid fragment having substantial sequence homology to said VNTR-containing fragment and a nucleic acid fragment which is capable of hybridizing to the single locus specified by pMHZ13.

23. A nucleic acid fragment selected from the group consisting of cYNA13, the VNTR-containing fragment of cYNA13, a nucleic acid fragment having substantial sequence homology to said VNTR-containing fragment and a nucleic acid fragment which is capable of hybridizing to the single locus specified by cYNA13.

24. A nucleic acid fragment selected from the group consisting of cYNA4, the VNTR-containing fragment of cYNA4, a nucleic acid fragment having substantial sequence homology to said VNTR-containing fragment and a nucleic acid fragment which is capable of hybridizing to the single locus specified by cYNA4.

25. A nucleic acid fragment selected from the group consisting of cKKA39, the VNTR-containing fragment of cKKA39, a nucleic acid fragment having substantial sequence homology to said VNTR-containing fragment and a nucleic acid fragment which is capable of hybridizing to the single locus specified by cKKA39.

26. A nucleic acid fragment selected from the group consisting of pMCT118, the VNTR-containing fragment of pMCT118, a nucleic acid having substantial sequence homology to said VNTR-containing fragment and a nucleic acid fragment which is capable of hybridizing to the single locus specified by pMCT118.

27. A nucleic acid fragment selected from the group consisting of pCMI327, the VNTR-containing fragment of pCMI327, a nucleic acid fragment having substantial sequence homology to said VNTR-containing fragment and a nucleic acid fragment which is capable of hybridizing to the single locus specified by pCMI327.

28. A nucleic acid fragment selected from the group consisting of pMCOB17, the VNTR-containing fragment of pMCOB17, a nucleic acid fragment having substantial sequence homology to said VNTR-containing fragment and a nucleic acid fragment which is capable of hybridizing to the single locus specified by pMCOB17.

29. A nucleic acid fragment selected from the group consisting of pRMU3, the VNTR-containing fragment of pRMU3, a nucleic acid fragment having substantial sequence homology to said VNTR-containing fragment and a nucleic acid fragment which is capable of hybridizing to the single locus specified by pRMU3.

30. A nucleic acid fragment selected from the group consisting of pEKMDA2I, the VNTR-containing fragment of pEKMDA2I, a nucleic acid fragment having substantial sequence homology to said VNTR-containing fragment and a nucleic acid fragment which is capable of hybridizing to the single locus specified by pEKMDA2I.

31. A nucleic acid fragment selected from the group consisting of cTBQ7, the VNTR-containing fragment of cTBQ7, a nucleic acid fragment having substantial sequence homology to said VNTR-containing fragment and a nucleic acid fragment which is capable of hybridizing to the single locus specified by cTBQ7.

32. A clone set which comprises the clones pYNH24, pCMM6, pCMM66, cCMM77, pCMM86, pMLJ14, cMLJ205, pYNZ22, pJCZ3.1, pJCZ16.2, pJCZ67, pYNZ21, pCMM101, cEFD64, cEFD52, pEFD139, pEFD126.3, pTHH33, pTHH59, cMHZ47, pMHZ10, pMHZ13, cYNA13, cYNA4, cKKA39, pMCT118, pCMI327, pEKMDA2I, pRMU3, pMCOB17, CTBQ7, nucleic acid fragments capable of hybridizing to the insert and nucleic acid fragments capable of hybridizing to the insert to the single locus specified thereby.

33. The clone set of claim 32 wherein said clones are pYNH24, pCMM6, pCMM66, cCMM77, pCMM86, pMLJ14, cMLJ205, pYNZ22, pJCZ3.1, pJCZ16.2, pJCZ67, pYNZ21, pCMM101, cEFD64, cEFD52, pEFD139, pEFD126.3, pTHH33, pTHH59, cMHZ47, pMHZ10, pMHZ13, cYNA13, cYNA4, cKKA39, pMCT118, pCMI327, pEKMDA2I, pRMU3, pMCOB17 and CTBQ7.

34. A clone set which comprises at least two of the clones selected from the group consisting of pYNH24, pCMM6, pCMM66, cCMM77, pCMM86, pMLJ14, cMLJ205, pYNZ22, pJCZ3.1, pJCZ16.2, pJCZ67, pYNZ21, pCMM101, cEFD64, cEFD52, pEFD139, pEFD126.3, pTHH33, pTHH59, cMHZ47, pMHZ10, pMHZ13, cYNA13, cYNA4, cKKA39, pMCT118, pCMI327, pEKMDA2I, pRMU3, pMCOB17, CTBQ7, nucleic acid fragments capable of hybridizing to the insert and nucleic acid fragments capable of hybridizing to the insert to the single locus specified thereby.

35. The clone set of claim 34 wherein said clones are more than 50 map units or cM apart.

36. The clone set of claim 34 wherein no more than one clone maps to any one chromosomal arm.

37. The clone set of claim 34 wherein no more than one clone maps to any one chromosome.

38. The clone set of claim 34 comprising pYNH24, pCMM101, pMLJ14, cTBQ7, pCMM66, pCMM73 and pCMM86.