×

Method of synthesizing oligonucleotides labeled with ammonia-labile groups on solid phase supports

  • US 4,965,349 A
  • Filed: 12/24/1987
  • Issued: 10/23/1990
  • Est. Priority Date: 12/24/1987
  • Status: Expired due to Term
First Claim
Patent Images

1. A process of cleaving a base-labile linking group between an oligonucleotide and a solid phase support comprising the step of exposing the linking group to a cleavage reagent until the linking group is hydrolyzed, the cleavage reagent comprising a lower alkyl alcohol, water, and a non-nucleophilic hindered alkylamine containing from 3 to 6 carbon atoms in a ratio of about 1:

  • 1;

    1 to about 1;

    3;

    1 by volume, respectively.

View all claims
  • 7 Assignments
Timeline View
Assignment View
    ×
    ×