Column for liquid chromatography

• US 4,968,421 A
• Filed: 09/14/1989
• Issued: 11/06/1990
• Est. Priority Date: 06/19/1981
• Status: Expired due to Fees

• Current Assignee: Ceskoslovenska akademie ved
• Original Assignee: Ceskoslovenska akademie ved
• Inventors: Vozka, Stanislav, Porsch, Bedrich, Kubin, Miroslav, Voslar, Jaroslav, Coupek, Jiri, Spacek, Pavel
• Primary Examiner(s): Therkorn, Ernest G.
• Application Number:
  US07/408,413
• Time in Patent Office: 418 Days
• Field of Search: 210/656, 210/198.2, 55/386, 422/70
• US Class Current: 210/198.2
• CPC Class Codes: G01N 30/6026 Fluid seals
• CPC Class Codes: G01N 30/6034 joining multiple columns
• CPC Class Codes: G01N 30/6039 in series
• CPC Class Codes: G01N 30/6082 transparent to radiation

Abstract

Column for liquid chromatography contains a glass tube for sorbent packing closed with simple column seals and inserted into a jacket made of metal or plastic material. The jacket transfers axial forces exerted on the column seals by liquid pressure action by means of sockets. It secures the tightness of the column, enables the connection of the column to a chromatographic system, and has a protective function at the same time. The columns can be coupled in series by means of doubled sockets.

Claims

1. A column for liquid chromatography comprising

a glass tube for sorbent packing,

a jacket surrounding the glass tube provided with connecting means on both ends, said jacket and glass tube forming an interspace so that said jacket does not come in contact with a mobile phase being analyzed thereby allowing the jacket to be made from metals of lower corrosion resistance,

a first bushing having an opening and positioned on the end of the glass tube and adapted to space apart the glass tube and the jacket,

said first bushing being adhesive bound to an outer portion of the end of the glass tube,

a second bushing comprising a seal located in the opening of the first bushing, said seal being a gasket and a permeable partition, and

removable sockets connected to each of said connecting means, each of said sockets including an element having a surface adapted to abut a respective seal, each of said sockets being adapted to cooperate with a respective connection means such that said element can exert a comprehensive pressure on a respective seal, and

each of said sockets including an opening for conduction of liquid, said opening communicating with the interior of the glass tube.

2. The column according to claim 1, wherein the glass tube has been reinforced by the surface diffusion of suitable ions.

3. The column according to claim 1, wherein the bushing is made of metal, has radial grooves in its inner wall, and is furnished inside with an insert made of plastic material, the insert having a bottom bearing on the face of the glass tube and containing the inserted permeable partition.

4. The column according to claim 1, wherein the bushing rests on the face of the glass tube and has a step around its opening, and a plug with the inserted permeable partition disposed in the step.

5. The column according to claim 1, wherein the jacket is made of metal selected from the group comprising stainless steel, aluminum and its alloys, brass and titanium, or of plastic material selected from the group comprising polyamides, polypropylene, polyvinyl chloride, polyester resins, phenol-formaldehyde resins and urea-formaldehyde resins.

6. The column according to claim 1, wherein a face member is separated from the socket, the socket being doubled-ended and furnished at both ends with threads adapted for connection with the jacket of another column.

7. The column according to claim 1, wherein the jacket is provided with openings for the inlet and outlet of thermo-regulating liquid medium.

8. The column according to claim 1, wherein the column jacket is furnished with one or several sight holes for visual observation of the column packing.

9. The column according to claim 1, wherein the bushing is fixed to the glass tube by means of a low-melting alloy having a melting point of 60 to 250 degrees C., which alloy consists of elements selected from the group comprising bismuth, lead, tin, cadmium, zinc and antimony.