Method and device for ketone measurements

• US 4,970,172 A
• Filed: 12/22/1986
• Issued: 11/13/1990
• Est. Priority Date: 12/22/1986
• Status: Expired due to Fees

• Current Assignee: Abbott Laboratories North Chicago IL
• Original Assignee: Abbott Laboratories
• Inventors: Kundu, Samar K.
• Primary Examiner(s): NOT, DEFINED
• Assistant Examiner(s): NOT, DEFINED
• Application Number:
  US06/944,083
• Time in Patent Office: 1,422 Days
• Field of Search: 436/130, 436/128, 436/169, 436/170, 422/58, 422/59, 422/60, 422/86, 422/87, 422/88, 422/85
• US Class Current: 436/130
• CPC Class Codes: A61B 5/083 Measuring rate of metabolism by using breath test, e.g. measuring rate of oxygen consumption
• CPC Class Codes: A61B 5/4872 Body fat
• CPC Class Codes: G01N 33/64 involving ketones
• CPC Class Codes: Y10T 436/202499 Formaldehyde or acetone

Abstract

The present invention relates to methods and materials for the detection of ketone and aldehyde analytes in fluid samples by means of reacting analyte containing samples with a first solid matrix material to which a nitroprusside salt is coupled and a second solid matrix material to which an amine is covalently coupled. Methods and devices are also provided for ascertaining the fat catabolism effects of a weight loss dietary regimen comprising determining the breath acetone concentration of the subject.

Claims

1. A method for the detection of a ketone or aldehyde analyte in a fluid sample comprising:

(a) contacting the analyte present in the sample with a first solid matrix material to which a nitroprusside salt is coupled and a second solid matrix material to which a primary or secondary amine is covalently coupled,

(b) reacting the analyte with the nitroprusside and the amine to form a detectable reaction product, and

(c) detecting the reaction product.

2. The method according to claim 1 wherein the nitroprusside salt is sodium nitroprusside.

3. The method according to claim 1 wherein the amine is a primary amine.

4. The method according to claim 1 wherein the fluid sample is a vapor.

5. The method according to claim 1 wherein the analyte is acetone.

6. The method according to claim 7 wherein the solvent comprises methanol and dimethylsulfoxide.

7. The method according to claim 1 wherein analytes are adsorbed onto an adsorbent material and are desorbed from the adsorbent material and contacted with the first and second solid matrix materials by means of a solvent.

8. The method according to claim 4 wherein analytes present in the vapor sample are adsorbed onto the first and second solid matrix materials.

9. The method according to claim 1 wherein the analyte concentration may be determined by detecting the extent to which analytes present in the sample fluid are linearly adsorbed onto the first and second matrix materials.

10. A device for the detection of ketones and aldehydes in a fluid sample comprising a first solid matrix material to which a nitroprusside salt is coupled and a second solid matrix material to which a primary or secondary amine compound is covalently bound.

11. The device according to claim 10 wherein said first and second solid matrix materials are immobilized on a strip.

12. The device according to claim 10 wherein said first and second solid matrix materials are immobilized in a column.

13. The device according to claim 10 wherein the nitroprusside salt is sodium nitroprusside.

14. The device according to claim 10 wherein the amine is a primary amine.

15. The device according to claim 10 wherein said first and second matrix materials are selected from the group consisting of silica gel materials and cellulosic materials.

16. The device according to claim 10 further comprising a preadsorbent zone.

17. The device according to claim 10 comprising means for isolation of a fixed volume of vapor in equilibrium with the liquid sample and means for contacting said fixed volume of vapor with the first and second solid matrix materials.

18. The device according to claim 10 wherein the nitroprusside salt and the amine are coupled to the same solid matrix material.

19. The device according to claim 10 comprising amino propyl silica gel and sodium nitroprusside coupled to diethylamino silica gel.