Process for preparing enzyme electrodes
First Claim
1. A process for preparing an enzyme electrode consisting essentially of the steps of:
- (a) applying an albumin solution containing 0.05 to 0.5% by weight of a multifunctional aldehyde onto the surface of an electroconductive substrate to form a coat comprised of said solution,(b) maintaining the atmospheric temperature of 0°
C. to 20°
C. to thereby form a permselective crosslinked albumin layer, and(c) applying a solution containing a hydrogen peroxide productive oxidase and greater than 0 up to 0.5% by weight of a multifunctional aldehyde onto the surface of the above permselective crosslinked albumin layer to form a hydrogen peroxide productive oxidase containing layer thereon.
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Abstract
An enzyme electrode is prepared by a process wherein an albumin solution containing 0.05 to 0.5% by weight of a multifunctional aldehyde is applied onto the surface of an electroconductive substrate while maintaining the atmospheric temperature at 0° to 20° C. to thereby form a crosslinked albumin layer on the surface of the electroconductive substrate. Thereafter a solution containing 0.5% by weight or less of a multifunctional aldehyde and a predetermined amount of an oxidase capable of producing hydrogen peroxide (H2 O2) is applied on the surface of the previously formed crosslinked albumin layer to form a H2 O2 productive oxidase containing layer.
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Citations
15 Claims
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1. A process for preparing an enzyme electrode consisting essentially of the steps of:
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(a) applying an albumin solution containing 0.05 to 0.5% by weight of a multifunctional aldehyde onto the surface of an electroconductive substrate to form a coat comprised of said solution, (b) maintaining the atmospheric temperature of 0°
C. to 20°
C. to thereby form a permselective crosslinked albumin layer, and(c) applying a solution containing a hydrogen peroxide productive oxidase and greater than 0 up to 0.5% by weight of a multifunctional aldehyde onto the surface of the above permselective crosslinked albumin layer to form a hydrogen peroxide productive oxidase containing layer thereon. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15)
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Specification