Reducible indicator compositions containing pyrogallol derivatives
First Claim
1. Composition useful in determining an analyte in a sample comprising (i) an indicator system containing a non-coupling chromogen and pyrogallol derivative or a pyrogallol ester and (ii) an enzyme reactive with the analyte to form a product which reacts with the indicator system to produce a detectable color signal, wherein said pyrogallol derivative or pyrogallol ester is present in an amount sufficient to prevent flattening of said detectable color signal over a range of analyte concentrations but is insufficient to stabilize said enzyme.
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Accused Products
Abstract
The invention relates to a composition useful in determining an analyte in a sample. The composition contains an indicator system which forms a detectable signal in the presence of said analyte, and which is not a coupling system, as well as a pyrogallol derivative. The pyrogallol derivative causes uniform formation of detectable signal. Also described are kits useful in determining an analyte, apparatus which incorporate the composition, and a method for determining an analyte using the composition.
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Citations
23 Claims
- 1. Composition useful in determining an analyte in a sample comprising (i) an indicator system containing a non-coupling chromogen and pyrogallol derivative or a pyrogallol ester and (ii) an enzyme reactive with the analyte to form a product which reacts with the indicator system to produce a detectable color signal, wherein said pyrogallol derivative or pyrogallol ester is present in an amount sufficient to prevent flattening of said detectable color signal over a range of analyte concentrations but is insufficient to stabilize said enzyme.
- 21. Kit useful in determining an analyte in a sample comprising a separate portion of each of a non-enzyme containing indicator system which forms a detectable color signal in the presence of a product formed by said analyte and an enzyme which reacts with said analyte, said indicator system comprising a non-coupling chromogen and a pyrogallol derivative or pyrogallol ester in an amount sufficient to prevent flattening of said detectable color signal over a range of analyte concentrations but is insufficient to stabilize said enzyme, (ii) an enzyme which reacts with said analyte to form said product, and (iii) a container means for holding separate portions (i) and (ii).
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23. Kit useful in determining an analyte in a sample, comprising a separate portion of each of (i) a non-enzyme containing indicator system which contains a non-coupling chromogen, (ii) an enzyme, which reacts with said analyte to form a product which reacts with the non-enzyme containing indicator system to produce a detectable color signal, that does not contain an indicator, a pyrogallol derivative or a pyrogallol ester, (iii) a non-enzyme containing pyrogallol derivative or pyrogallol ester in an amount sufficient to prevent flattening of said detectable color signal over a range of analyte concentrations but is insufficient to stabilize said enzyme, and (iv) a container means for holding each of portions (i), (ii) and (iii).
Specification