Signal processing method for determining base sequence of nucleic acid
First Claim
1. A signal processing method for determining base sequence of nucleic acids by subjecting to signal processing digital signals corresponding to an autoradiograph of plural resolved rows which are formed by resolving a mixture of base-specific DNA fragments or base-specific RNA fragments labeled with a radioactive element in a one-dimensional resolving direction on a support medium, which comprises the steps of:
- (1) generating a one-dimensional waveform composed of position along the resolving direction and signal level for each resolved row;
(2) selecting at least two intervals along the one-dimensional waveform;
(3) subjecting the digital signals in each said interval to smoothing processing using a moving average filter having a mask size that is varied for each said interval; and
(4) subjecting the smoothing processed digital signals to further processing, thereby detecting and sequencing bands corresponding to the sequence of bases in said nucleic acids.
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Abstract
A signal processing method for determining base sequence of nucleic acids by subjecting digital signals to signal processing, said digital signals corresponding to an autoradiograph of plural resolved rows which are formed by resolving a mixture of base-specific DNA fragments or base-specific RNA fragments labeled with a radioactive element in one-dimensional direction on a support medium,
which comprises steps of:
(1) preparing one-dimensional waveform composed of position along the resolving direction and signal level for each resolved row;
(2) dividing the one-dimensional waveform into at least two intervals; and
(3) smoothing the one-dimensional waveform in every interval through a smoothing means having different characteristics.
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Citations
12 Claims
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1. A signal processing method for determining base sequence of nucleic acids by subjecting to signal processing digital signals corresponding to an autoradiograph of plural resolved rows which are formed by resolving a mixture of base-specific DNA fragments or base-specific RNA fragments labeled with a radioactive element in a one-dimensional resolving direction on a support medium, which comprises the steps of:
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(1) generating a one-dimensional waveform composed of position along the resolving direction and signal level for each resolved row; (2) selecting at least two intervals along the one-dimensional waveform; (3) subjecting the digital signals in each said interval to smoothing processing using a moving average filter having a mask size that is varied for each said interval; and (4) subjecting the smoothing processed digital signals to further processing, thereby detecting and sequencing bands corresponding to the sequence of bases in said nucleic acids. - View Dependent Claims (2, 3, 4, 5, 6)
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7. A signal processing method for determining base sequence of nucleic acids by subjecting to signal processing digital signals corresponding to an autoradiograph of plural resolved rows which are formed by resolving a mixture of base-specific DNA fragments or base-specific RNA fragments labeled with a radioactive element in a one-dimensional resolving direction on a support medium, which comprises the steps of:
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(1) generating a one-dimensional waveform composed of position along the resolving direction and signal level for each resolved row; (2) selecting at least two intervals in the resolving direction along the one-dimensional waveform thus generated; (3) processing each of said intervals with a smoothing means for smoothing processing the digital signals in each said interval according to spaces of bands in each said interval; (4) detecting the sequence of bands in each resolved row; and (5) determining said base sequence from the detected band sequence. - View Dependent Claims (8, 9, 10, 11, 12)
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Specification