Dental caries diagnostic and localization technique
First Claim
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1. A method for detecting the presence, extent and location of a specified microorganism on a surface, comprising:
- (a) contacting the surface with a solid substrate containing at least one substance essential for the growth of the specified microorganism;
(b) removing said solid substrate from the surface and immersing it in a liquid growth medium lacking said at least one substance essential for the growth of the specified microorganism but otherwise having the necessary growth ingredients for growing the specified microorganism; and
(c) incubating said liquid growth medium with the solid substrate immersed therein to promote the growth of the specified microorganism at the interface between the solid substrate and the liquid medium.
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Abstract
A method for detecting and localizing bacterial growth on a surface comprising the steps of:
(a) applying a solid substrate to the surface;
(b) removing said substrate from said surface and incubating said substrate for purposes of facilitating ultimate visualization of selective bacterial growth; and
(c) observing the locus of bacterial growth on the substrate.
The method is particularly effective in detecting and localizing cariogenic activity in the mouth.
23 Citations
19 Claims
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1. A method for detecting the presence, extent and location of a specified microorganism on a surface, comprising:
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(a) contacting the surface with a solid substrate containing at least one substance essential for the growth of the specified microorganism; (b) removing said solid substrate from the surface and immersing it in a liquid growth medium lacking said at least one substance essential for the growth of the specified microorganism but otherwise having the necessary growth ingredients for growing the specified microorganism; and (c) incubating said liquid growth medium with the solid substrate immersed therein to promote the growth of the specified microorganism at the interface between the solid substrate and the liquid medium. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11)
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12. A method for detecting the presence of one or more specified microorganisms in an examined medium comprising:
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(a) effecting contact between said examined medium and a solid substrate containing at least one substance essential for the growth of the specified microorganism; (b) immersing said solid substrate in a liquid growth medium lacking said at least one substance essential for the growth of the specified microorganism but otherwise having the necessary growth ingredients for growing the specified microorganism; and (c) incubating said liquid growth medium with the solid substrate immersed therein to promote the growth of the specified microorganism at the interface between the solid substrate and the liquid medium. - View Dependent Claims (13, 14, 15, 16, 17, 18, 19)
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Specification