Cuvette for receiving liquid sample

• US 4,979,821 A
• Filed: 08/01/1989
• Issued: 12/25/1990
• Est. Priority Date: 01/27/1988
• Status: Expired due to Term

• Current Assignee: Ortho Diagnostic Systems, Inc. (The Carlyle Group LP (Corporate Private Equity))
• Original Assignee: Ortho Diagnostic Systems, Inc. (The Carlyle Group LP (Corporate Private Equity))
• Inventors: Schutt, Ernest G., Utberg, Karin L., Greenwood, David L.
• Primary Examiner(s): McGraw, Vincent P.
• Application Number:
  US07/388,193
• Time in Patent Office: 511 Days
• Field of Search: 356/318, 356/246, 356/440, 250/458.1, 250/459.1, 250/461.1, 250/461.2
• US Class Current: 356/246
• CPC Class Codes: G01J 3/0216 using light concentrators or collectors or condensers
• CPC Class Codes: G01J 3/0243 having a through-hole enabling the optical element to fulfil an additional optical function, e.g. a mirror or grating having a throughhole for a light collecting or light injecting optical fiber
• CPC Class Codes: G01J 3/0297 Constructional arrangements for removing other types of optical noise or for performing calibration
• CPC Class Codes: G01N 21/21 Polarisation-affecting properties G01N21/19 takes precedence
• CPC Class Codes: G01N 21/47 Scattering, i.e. diffuse reflection G01N21/25, G01N21/41 take precedence G01N21/55 takes precedence
• CPC Class Codes: G01N 21/552 Attenuated total reflection
• CPC Class Codes: G01N 33/553 Metal or metal coated
• CPC Class Codes: G01N 33/585 with a particulate label, e.g. coloured latex
• CPC Class Codes: Y10S 436/807 Apparatus included in process claim, e.g. physical support structures

Abstract

A new immunoassay system is provided for the detection of ligands or ligand binding partners in solution in a heterogeneous format. The invention relies upon the detection of back scattered light from an evanescent wave disturbed by the presence of a colloidal gold label brought to the interface by an immunological reaction. The evanescent wave existing at the interface in turn is the result of a totally internally reflected incident light wave. Placement of the detector at a back angle above the critical angle insures a superior signal-to-noise ratio. Apparatus and methods for scanning, detecting and manipulating light including a scattered total internal reflectance immunoassay system are provided.

Claims

1. A cuvette for receiving a liquid sample containing light-scattering molecules, said cuvette having:

a well for said liquid sample having at least one surface of optically transparent material adapted to be contacted by said liquid sample,

said surface when contacted by said liquid sample adapted to reflect a light beam striking said surface and adapted to emit elastically scattered light,

a face portion adapted to admit a light beam to strike said surface and further adapted to release said elastically scattered light through said face portion, and

a light-trap prism adjacent said surface to absorb reflected light energy by multiple reflections.

2. The cuvette of claim 1 wherein said surface of optically transparent material is a planar surface.

3. The cuvette of claim 2 wherein said face portion is located at the base of the cuvette.

4. The cuvette of claim 3 wherein the face portion is adapted to admit a light beam to strike said planar surface at about six degrees above the critical angle.

5. The cuvette of claim 1 wherein the light-trap prism has upper and lower planar prism surfaces which are substantially parallel, said upper planar prism surface adapted to emit reflected light.

6. A cuvette of molded optically transparent material for receiving a liquid sample containing light-scattering molecules, said cuvette having:

a well for said liquid sample having at least one planar surface adapted to be contacted by said liquid sample,

said planar surface when contacted by said liquid sample adapted to reflect a light beam striking said surface and adapted to emit elastically scattered light,

a face portion at the base of said cuvette adapted to admit a light beam to strike said surface and further adapted to release said elastically scattered light through said face portion, and

a light-trap prism adjacent said planar surface and above said face portion having upper and lower prism surfaces which are substantially parallel, said upper planar prism surface adapted to transmit a portion of said light beam reflected from said planar surface, said prism further adapted to absorb a portion of said reflected light beam energy by multiple reflections.