Cryoprotectant solution and method
First Claim
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1. A cryoprotectant solution for viable cells which comprises an aqueous suspending vehicle which is physiologically acceptable to the cells and contains cryoprotectant additives and betaine in an amount of about 25% by weight of said solution.
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Abstract
This invention relates to an improved cryoprotectant solution for viable cells, such as sperm, ova and blood cells which contains an effective amount of betaine.
103 Citations
6 Claims
- 1. A cryoprotectant solution for viable cells which comprises an aqueous suspending vehicle which is physiologically acceptable to the cells and contains cryoprotectant additives and betaine in an amount of about 25% by weight of said solution.
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3. A cryoprotectant solution for viable cells which is physiologically acceptable to the cells which comprises betaine in an amount of about 25% by weight of said solution and additionally contains lactose, egg yolk, glycerol, glucose, ethylene diamine tetracetic acid, sodium bicarbonate, trisodium citrate-2-hydrate, distilled water and penicillin.
- 4. A method for cryopreservation of viable cells which comprises the steps of maintaining the cells at a cryogenic temperature in an aqueous suspending vehicle which is physiologically acceptable to the cells, said vehicle containing betaine in an amount of about 25% by weight.
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Current AssigneeFinnfeeds Finland Oy (International Flavors & Fragrances Incorporated)
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Original AssigneeCultor Limited
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InventorsJunnila, Matti A. J.
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Primary Examiner(s)Rosen, Sam
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Application NumberUS07/109,495Time in Patent Office1,166 DaysField of Search435/2, 435/1, 62/64, 128/DIG. 27, 252/403US Class Current435/2CPC Class CodesA01N 1/02 Preservation of living partsA01N 1/0221 Freeze-process protecting a...
