Multiple port assay device
First Claim
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1. A device for conducting an assay, said device comprising:
- (a) a housing,(b) means enclosed in said housing for capturing a first member of a specific binding pair (sbp) in a zone and for allowing liquid to be transported by capillary action away from said zone,(c) first inlet means in said housing for introducing a sample into the device, and(d) second inlet means in said housing for introducing liquid reagent other than said sample into the device without also introducing said liquid reagent through said first inlet means.a portion of said means for capturing being in liquid receiving relationship with said first inlet means and said second inlet means.
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Abstract
A device is disclosed for conducting an assay. The device comprises a housing, means enclosed in the housing for capturing a first member of a specific binding pair (sbp) in a zone and for allowing liquid to be transported by capillary action away from the zone, first means in the housing for introducing the sample into the device, and second means in the housing other than the first means for introducing a liquid reagent other than the sample into the device. The device of the invention finds use in assay methods for the determination of an analyte in a sample suspected of containing the analyte. Kits for conducting an assay are also disclosed.
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Citations
52 Claims
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1. A device for conducting an assay, said device comprising:
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(a) a housing, (b) means enclosed in said housing for capturing a first member of a specific binding pair (sbp) in a zone and for allowing liquid to be transported by capillary action away from said zone, (c) first inlet means in said housing for introducing a sample into the device, and (d) second inlet means in said housing for introducing liquid reagent other than said sample into the device without also introducing said liquid reagent through said first inlet means. a portion of said means for capturing being in liquid receiving relationship with said first inlet means and said second inlet means. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 48)
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23. A device for conducting an assay, which device comprises:
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a housing, a first opening in said housing for introducing a sample suspected of containing an analyte into said device, bibulous material enclosed in said housing, said bibulous material being capable of transporting liquid away from the point of introduction of said sample, and a second opening in said housing for introducing liquid reagent other than said sample into said device without contacting said bibulous material at said point of introduction with said liquid reagent except by capillary transport, a portion of said bibulous material being in liquid receiving relationship with said first opening and said second opening. - View Dependent Claims (24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39, 50, 51, 52)
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40. A device for conducting an immunoassay for an analyte in a sample suspected of containing said analyte, which device comprises:
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a housing having means for viewing the interior thereof, first inlet means for introducing a sample therein, and second inlet means for introducing therein an aqueous solution other than said sample, and a piece of bibulous material enclosed in said housing, said bibulous material having a specific binding pair (sbp) member non-diffusively bound to a situs on said bibulous material, said bibulous material further having a labeled sbp member diffusively deposited on said bibulous material between said means for viewing and said second inlet means. a portion of said bibulous material being in liquid receiving relationship with said first inlet means and said second inlet means and said situs being in viewing relationship with said means for viewing. - View Dependent Claims (41, 42, 43)
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- 44. In a device for conducting an assay, said device comprising inlet means for introducing a sample containing an analyte into said device and means for transporting liquid away from said inlet means, the improvement which comprises second inlet means other than said inlet means for introducing liquid reagent other than said sample into said device and means for transporting said liquid reagent by capillaRy action away from said second inlet means, a portion of said means for transporting being in liquid receiving relationship with said inlet means and said second inlet means.
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49. In a method for conducting an assay for an analyte in a sample suspected of containing said analyte, which method comprises:
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introducing a test solution comprising said sample onto a bibulous material in an assay device through inlet means and allowing said test solution to traverse at least a portion of said bibulous material contained in said device, introducing into said device immediately thereafter a liquid agent to traverse at least a portion of said bibulous material, and observing said bibulous material for the presence of a signal in relation to the presence of analyte in said sample, the improvement which comprises introducing said liquid agent into said device at a second inlet means such that said liquid agent does not also enter said device through said first inlet means.
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Specification