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Process of producing highly transformable cells and cells produced thereby

DC
  • US 4,981,797 A
  • Filed: 11/14/1988
  • Issued: 01/01/1991
  • Est. Priority Date: 08/08/1985
  • Status: Expired due to Term
First Claim
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1. A process for producing transformable E. coli cells of improved competence by a process comprising the following steps in order:

  • (a) growing E. coli cells in a growth-conducive medium at a temperature of 18°

    C. to 32°

    C.;

    (b) rendering said E. coli cells competent; and

    (c) freezing the cells.

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