Nucleic acid probes of human papilloma virus
First Claim
1. Nucleic acid probe useful for detecting indifferently eight types of human papilloma virus, HPV1a, HPV5, HPV6b HPV8, HPV11, HPV16, HPV18 and HPV33, consisting of a sequence of labelled nucleic acids, said probe consisting essentially of a mixture of oligomers, which include a sequence of 12 nucleotides X-A-A-A-A-C-G-A-A-A-G-X with X=T or U, or its complement extended on each side by 1 to 10 corresponding nucleotides for each of the eight virus types.
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Abstract
The present invention relates to probes of nucleic acids useful for detecting indifferently the various types of human papilloma virus, particularly HPV1a, HPV5, HPV6b, HPV8, HPV11, HPV16, HPV18 and HPV33, especially a probe comprising a labelled sequence of nucleic acids, characterized in that it comprises the oligomer of twelve nucleotides X-A-A-A-A-C-G-A-A-A-G-X, with X=T or U, or its complement by interchanging A and X on the one hand, C and G on the other hand. The present invention also relates to specific probes of nucleic acids for the detection of human papilloma for each of the types HPV1a, HPV5, HPV8, HPV11, HPV16, HPV18 and HPV33, as well as specific probes of sub-groups of the virus HPV16, HPV18, HPV33 or HPV16 and HPV18 only or again HPV5 and HPV8 only.
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Citations
17 Claims
- 1. Nucleic acid probe useful for detecting indifferently eight types of human papilloma virus, HPV1a, HPV5, HPV6b HPV8, HPV11, HPV16, HPV18 and HPV33, consisting of a sequence of labelled nucleic acids, said probe consisting essentially of a mixture of oligomers, which include a sequence of 12 nucleotides X-A-A-A-A-C-G-A-A-A-G-X with X=T or U, or its complement extended on each side by 1 to 10 corresponding nucleotides for each of the eight virus types.
- 2. Nucleic acid probe common to the eight different types of HPV virus, HPV1a, HPV5, HPV6b, HPV8, HPV11, HPV16, HPV18 and HPV33, said probe consisting essentially of a mixture of six oligomers, which include a common sequence of twelve nucleotides X-A-A-A-A-C-G-A-A-A-G-X and with X=T or U, or its complement extended on each side by 1 to 10 corresponding nucleotides for each of the eight virus types.
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4. Specific probe of nucleic acids for the detection of the HPV1a type of human papilloma virus, said probe consisting essentially of an oligomer selected from among:
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CCXCAGGCGAGAAGCGCGGAC CXGXCCCXXCXGCGCGAGACGX XGXCCCXXCXGCGCGAGACGXXGGAAGXAXACACA GACCGXCCXCGCGGAXCACAG GACCGXCCXCGCGGAXCACAGCGCCAXXAGACAGC with X=T or U and their complements.
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5. Specific nucleic acid probe for the detection of the HPV5 type of human papilloma virus, said probe consisting essentially of an oligomer selected from among:
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XGXAXGGGAXCGGGGGCGXXXA XXGXAXGGGAXCGGGGGCGXXXA XGXAXGGGAXCGGGGGCGXXXAGCCAXGGACCAXA GCCXGACAACGAAAGGAXCXCXXA GAGGAGCCXGACAACGAAAGGAXCXCXXACAAAGX with X=T or U and their complements.
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6. Specific probe of nucleic acids for the detection of the HPV6b type of human papilloma virus, said probe consisting essentially of an oligomer selected from among:
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CAXXAACGCAGGGGCGCCXGAA GGCAAXCAXXAACGCAGGGGCG GCAAXCAXXAACGCAGGGGCGCCXGAAAXXGXGCC GACGAAGXGGACGGACAAGAXXC GAGGXGGACGAAGXGGACGGACAAGAXXCACAACC with X=T or U and their complements.
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7. Specific probe of nucleic acids for the detection of the HPV8 type of human papilloma virus, said probe consisting essentially of an oligomer selected from among:
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ACACCGCCGCCAAGACCCCCA GCAGCXCAGXCCGCGGCXCC GCAGCXCAGXCCGCGGCXCCAGXCAAXAXCACXGX XGXCAACGCAACXGAXXCGGGXAX CXXXXXGXCAACGCAACXGAXXCGGGXAXCAGGAC with X=T or U and their complements.
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8. Specific probe of nucleic acids for the detection of the HPV11 type of human papilloma virus, said probe consisting essentially of an oligomer selected from among:
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GGCGXGXCGGCGCCGCCXAG GAAGCXCXCCCAAGCCXGCXAXX XAXAXACCCXXGGGAAGCXCXCCCAAGCCXGCXAX XGAGGXGGACAAGGXGGACAAAC AGAAGAXGAGGXGGACAAGGXGGACAAACAAGACG with X=T or U and their complements.
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9. Specific probe of nucleic acids for the detection of the HPV16 type of human papilloma virus, said probe consisting essentially of an oligomer selected from among:
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CAGAACCGGACAGAGCCCAX ACCAGAGACAACXGAXCXCXACXGX and ACAAGCAGAACCGGACAGAGCCCA XGXXAGAXXXGCAACCAGAGACAACXGAXCXCXAC AGXGXGACXCXACGCXXCGGXXG XXGCAAGXGXGACXCXACGCXXCGGXXGXGCGXAC with X=T or U and their complements.
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10. Specific probe of nucleic acids for the detection of the HPV18 type of human papilloma virus, said probe consisting essentially of an oligomer selected from among:
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CGGXAXCCGCXACXCAGCXXG CCCAXXGXAXCACCCACGGCCC CCCAXXGXAXCACCCACGGCCCCXGCCXCXACACA XXGXAAGXGXGAAGCCAGAAXXGAG AXGXGXXGXAAGXGXGAAGCCAGAAXXGAGCXAGX with X=T or U and their complements.
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11. Specific probe of nucleic acids for the detection of the HPV33 type of human papilloma virus, said probe consisting essentially of an oligomer selected from among:
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CGXCCXCCGGXXACXGXAGAXA GXCCXCCGGXXACXGXAGACACX XCCXCCGGXXACXGXAGACACXGXXGGACCXXXAG AGCAAGXGACCXACGAACCAXACA AGXACAGCAAGXGACCXACGAACCAXACAGCAACX with X=T or U and their complements.
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12. Specific probe of nucleic acids of the subgroup of HPV virus constituted by HPV16, HPV18 and HPV33, said probe consisting essentially of the oligomer
XXAGGCACAXAXXXX with X=T or U or its complement.
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13. Specific probe of nucleic acids of the subgroup of HPV virus constituted by HPV16 and HPV 18, said probe consisting essentially of the oligomer
AXAXCAAAXAXXAGXGAAGX with X=T or U or its complement.
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14. Specific probe of nucleic acids of the subgroup of HPV virus constituted by HPV5 and HPV8, said probe consisting essentially of the oligomer
CAAACAACAGXXGCXGACAAXAXXXXAAAAXAXGGCAGXGCXGGXGXAXXX XXXGG with X=T or U or its complement or a fragment of the latter comprising between 18 and 35 oligomers such as XAXGGCAGXGCXGGXGXAXXXXXXG with X=T or U or its complement.
Specification