Device for carrying out a heterogeneous reaction

• US 4,985,204 A
• Filed: 02/23/1988
• Issued: 01/15/1991
• Est. Priority Date: 03/02/1987
• Status: Expired due to Fees

• Current Assignee: Boehringer Mannheim GmbH (Roche Holding AG)
• Original Assignee: Boehringer Mannheim GmbH (Roche Holding AG)
• Inventors: Erler, Klaus, Klose, Sigmar, Uhl, Wolfgang, Baier, Manfred
• Primary Examiner(s): Wax, Robert A.
• Assistant Examiner(s): Beisner, William H.
• Application Number:
  US07/159,253
• Time in Patent Office: 1,057 Days
• Field of Search: 422/58, 422/61, 422/69, 422/72, 422/101, 422/55, 422/56, 422/57, 422/103, 436/165, 436/169, 436/170, 436/808, 436/824, 436/825, 436/807, 436/809, 436/810, 435/7, 435/30, 435/288, 435/299-301, 435/810
• US Class Current: 422/412
• CPC Class Codes: B01L 3/502 with fluid transport, e.g. in multi-compartment structures centrifugal-type cuvettes G01N21/07; analysis by separation into components G01N30/00; automatic analysers G01N35/00
• CPC Class Codes: G01N 33/54366 Apparatus specially adapted for solid-phase testing

Abstract

The present invention provides a device for carrying out a heterogeneous reaction for the determination of a component of a sample liquid on a first capillary action carrier present in a measurement chamber, to which carrier adheres at least one non-soluble reagent, wherein at least one soluble reagent is present on a second capillary action carrier in a first pre-chamber, which carrier is in capillary contact with the capillary action carrier in the measurement chamber, which first pre-chamber is connected via a first capillary with a first application chamber to be filled from outside through a filling opening and the measurement chamber is also connected with a waste chamber by a capillary arrangement through which can only flow a liquid under a predetermined gravitational force into the measurement chamber.

Claims

1. Device for determining a component of a sample liquid, said device comprising:

a measurement chamber containing a first capillary action carrier and a first reagent, which is non-soluble in said sample liquid, adhering to said first capillary action carrier,

a first pre-chamber containing a second capillary action carrier and a second reagent, which is soluble in said sample liquid, adhering to said second carrier, said second carrier being in bodily capillary contact with said first carrier, said first pre-chamber lying above said measurement chamber in a first working position of said device,

a first application chamber having a first filling opening for introduction of said sample liquid and which, in said first working position of said device, lies above said first pre-chamber,

a first capillary means connecting said first application chamber to said first pre-chamber,

a waste chamber which lies below said measurement chamber in a second working position of said device,

gravitational capillary means between said measurement chamber and said waste chamber and permitting flow of liquid from said measurement chamber to said waste chamber only under a predetermined gravitational force, and

a second application chamber having a second filling opening for introduction of liquid, said second application chamber being connected via a second capillary means with a second pre-chamber, said second pre-chamber containing at least one indicator reagent on a third capillary action carrier, said second pre-chamber being connected via a third capillary means with a mixing chamber, said mixing chamber being connected via a fourth capillary means with said measurement chamber.

2. Device according to claim 1, wherein said mixing chamber comprises a nose projection.

3. Device according to claim 2 wherein said second capillary means starts from a point of said second application chamber which lies at about the middle of the distance between said second filling opening and the bottom of said chamber lying opposite said second filling opening.

4. Device according to claim 1 wherein said capillary means have hydraulic diameters of 0.04 to 2 mm.

5. Device according to claim 1, wherein said capillary means have hydraulic diameters of 0.2 to 0.6 mm.

6. Device according to claim 1 wherein said capillary action carriers consist of a material selected from the group consisting of paper, fleece, fabric, woven textile and porous membrane material.

7. Device according to claim 1 wherein at least one of said waste chamber and said mixing chamber comprises at least one ventilation opening.

8. Device according to claim 1 further comprising a fourth pre-chamber containing a capillary action carrier which connects said second application chamber with said first pre-chamber.

9. Device according to claim 8, wherein said capillary action carrier consist of a material selected from the group consisting of paper, fleece, fabric, woven textile and porous membrane material.

10. Device according to claim 1, further comprising a first plate with a surface into which said device is incorporated, said first plate being covered by a second plate, wherein at least one of said plate is transparent at least in the region of said measurement chamber.

11. Device of claim 10 wherein said plates comprise a material selected from the group consisting of glass and synthetic resin.

12. Device of claim 11, wherein said synthetic resin is polyacrylate or polystyrene.

13. Device according to claim 1, wherein said second capillary means connects with said second pre-chamber via a static, capillary flow one way valve, said valve permitting flow only into said second pre-chamber.

14. Device according to claim 13, further comprising a first plate with a surface into which said device is incorporated, said first plate being covered by a second plate, wherein at least one of said plate is transparent at least in the region of said measurement chamber.

15. Device of claim 14 wherein said plates comprise a material selected from the group consisting of glass and synthetic resin.

16. Device of claim 15, wherein said synthetic resin is polyacrylate or polystyrene.

17. Device according to claim 1, wherein said fourth capillary means connects with said measurement chamber via a static, capillary flow one-way valve, said valve permitting flow only into said measurement chamber.

18. Device according to claim 17, further comprising a first plate with a surface into which said device is incorporated, said first plate being covered by a second plate, wherein at least one said plate is transparent at least in the region of said measurement chamber.

19. Device of claim 18 wherein said plates comprise a material selected from the group consisting of glass and synthetic resin.

20. Device of claim 19, wherein said synthetic resin is polyacrylate or polystyrene.

21. Device according to claim 1 further comprising a third pre-chamber which lies in series with said second pre-chamber which third pre-chamber contains at least one adjuvant reagent on a fourth capillary action carrier.

22. Device according to claim 21, wherein said capillary action carriers consist of a material selected from the group consisting of paper, fleece, fabric, woven textile and porous membrane material.

23. Device according to claim 21, further comprising a first plate with a surface into which said device is incorporated, said first plate being covered by a second plate, wherein at least one of said plate is transparent at least in the region of said measurement chamber.

24. Device of claim 23 wherein said plates comprise a material selected from the group consisting of glass and synthetic resin.

25. Device of claim 24, wherein said synthetic resin is polyacrylate or polystyrene.

26. Device according to claim 1, further comprising a fifth capillary means connecting said second application chamber with said measurement chamber.

27. Device according to claim 26 wherein said fifth capillary means connects to said second application chamber at a height about equal to the height of the filling opening of said second application chamber.

28. Device according to claim 26, wherein a separating wall divides said second application chamber into two regions, a first of which connects with said second capillary means and said second filling opening, and a second of which connects with said fifth capillary means, said wall being less than complete, whereby said regions can be in fluid communication.

29. Device according to claim 26, wherein said capillary means have hydraulic diameters of 0.04 to 2 mm.

30. Device according to claim 26, wherein said capillary means have hydraulic diameters of 0.2 to 0.6 mm.

31. Device according to claim 26, further comprising a first plate with a surface into which said device is incorporated, said first plate being covered by a second plate, wherein at least one of said plates is transparent at least in the region of said measurement chamber.

32. Device of claim 31 wherein said plates comprise a material selected from the group consisting of glass and synthetic resin.

33. Device of claim 32, wherein said synthetic resin is polyacrylate or polystyrene.

34. Device according to claim 1, wherein said fifth capillary means connects with said measurement chamber via a static, capillary flow one-way valve, said valve permitting flow only into said measurement chamber.

35. Device according to claim 34, further comprising a first plate with a surface into which said device is incorporated, said first plate being covered by a second plate, wherein at least one of said plate is transparent at least in the region of said measurement chamber.

36. Device of claim 35 wherein said plates comprise a material selected from the group consisting of glass and synthetic resin.

37. Device of claim 36, wherein said synthetic resin is polyacrylate or polystyrene.

38. Device for determining a component of a sample liquid, said device comprising:

a measurement chamber containing a first capillary action carrier and a first reagent, which is non-soluble in said sample liquid, adhering to said first capillary action carrier,

a first pre-chamber containing a second capillary action carrier and a second reagent, which is soluble in said sample liquid, adhering to said second carrier, said second carrier being in bodily capillary contact with said first carrier, said first pre-chamber lying above said measurement chamber in a first working of said device,

a first application chamber having a first filling opening for introduction of said sample liquid and which, in said first working position of said device, lies above said first pre-chamber,

a first capillary means connecting said first application chamber to said first pre-chamber,

a waste chamber which lies below said measurement chamber in a second working position of said device, and

gravitational capillary means between said measurement chamber and said waste chamber and permitting flow of liquid from said measurement chamber to said waste chamber only under a predetermined gravitational force,

wherein said second working position is different from said first working position, said gravitational capillary means directly connecting said first pre-chamber with said waste chamber, whereby in said second working position, said liquid flows from said measurement chamber through said first capillary means and said gravitational capillary means.

39. Device according to claim 38, wherein said waste chamber comprises at least one ventilation opening.

40. Device according to claim 38, wherein said capillary means have hydraulic diameters of 0.04 to 2 mm.

41. Device according to claim 38 wherein said capillary means have hydraulic diameters of 0.2 to 0.6 mm.

42. Device according to claim 38, wherein said capillary action carriers consist of a material selected from the group consisting of paper, fleece, fabric, woven textile and porous membrane material.

43. Device according to claim 38 wherein said measurement chamber, first pre-chamber, first application chamber, waste chamber, and said gravitational capillary means are incorporated into a surface of a first plate which is covered by a second plate, wherein at least one of said plates is transparent at least in the region of said measurement chamber.

44. Device according to claim 43, wherein said plates comprise a material selected from the group consisting of glass and synthetic resin.

45. Device according to claim 44, wherein said synthetic resin is polyacrylate or polystyrene.

46. Device according to claim 38, wherein said first capillary means connects with said pre-chamber via a static, capillary flow one-way valve, said valve permitting flow only into said first pre-chamber.

47. Device according to claim 46, further comprising a first plate with a surface into which said device is incorporated, said first plate being covered by a second plate, wherein at least one of said plate is transparent at least in the region of said measurement chamber.

48. Device of claim 47 wherein said plates comprise a material selected from the group consisting of glass and synthetic resin.

49. Device of claim 48, wherein said synthetic resin is polyacrylate or polystyrene.