Method of making an enzyme membrane for enzyme electrodes
First Claim
1. A method for preparing a composite, multilayer membrane, which comprises uniformly dispersing one or more enzymes in a liquid solution of a polyurethane, applying droplets of the solution with the dispersed enzyme therein to a first cellulose membrane to form an enzyme film layer, drying the so applied solution, and bonding with a liquid adhesive a second cellulose membrane to the dried solution.
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Abstract
The inventive membrane of high selectivity comprises a laminated enzyme membrane, which is enclosed by two cellulose membranes. The laminated enzyme membrane is an enzyme-containing polyurethane layer; the cellulose membranes are coated with cellulose nitrate.
The inventive process is characterized in that ferricyanide or hydrogen peroxide is added in the presence of peroxidase to the measurement sample, the measurement portion or the enzyme membrane.
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Citations
8 Claims
- 1. A method for preparing a composite, multilayer membrane, which comprises uniformly dispersing one or more enzymes in a liquid solution of a polyurethane, applying droplets of the solution with the dispersed enzyme therein to a first cellulose membrane to form an enzyme film layer, drying the so applied solution, and bonding with a liquid adhesive a second cellulose membrane to the dried solution.
Specification