Magnetic separation device and methods for use in heterogeneous assays
First Claim
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1. A magnetic separation apparatus for use in assays employing solid phases comprising magnetic particles, said apparatus comprising:
- (a) base means having a plurality of orifices for receiving nonferrous containers adapted to contain said magnetic particles; and
(b) a plurality of magnet means mounted on said base and spaced about the periphery of each receiving orifice wherein each of said magnet means possess a north-south magnetic field orientation in a direction which is coplanar with a cross-sectional plane through said receiving orifices and wherein each of said north-south magnetic fields of said magnet means are oriented in a common direction and wherein said magnet means are mounted in a fixed relation with respect to themselves and to said base whereby each nonferrous container, when placed in receiving relationship with each of said orifices, has four magnet means about its periphery.
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Abstract
Magnetic separation devices are described for use in immunoassay or hybridization assay procedures. The most preferred embodiments comprise a defined relationship between microtube and microtiter plate receiving orifices and rare earth cobalt magnets having predetermined magnetic field orientations.
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Citations
17 Claims
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1. A magnetic separation apparatus for use in assays employing solid phases comprising magnetic particles, said apparatus comprising:
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(a) base means having a plurality of orifices for receiving nonferrous containers adapted to contain said magnetic particles; and (b) a plurality of magnet means mounted on said base and spaced about the periphery of each receiving orifice wherein each of said magnet means possess a north-south magnetic field orientation in a direction which is coplanar with a cross-sectional plane through said receiving orifices and wherein each of said north-south magnetic fields of said magnet means are oriented in a common direction and wherein said magnet means are mounted in a fixed relation with respect to themselves and to said base whereby each nonferrous container, when placed in receiving relationship with each of said orifices, has four magnet means about its periphery. - View Dependent Claims (2, 3, 4, 5)
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6. A magnetic separation apparatus for use in assays employing solid phases comprising magnetic particles, said apparatus comprising:
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(a) a base means having a plurality of orifices for receiving nonferrous containers adapted to contain said magnetic particles; and (b) a plurality of magnet means mounted on said base spaced about the periphery of each receiving orifice and wherein said magnet means are mounted in a fixed relation with respect to themselves and to said base and wherein each of said magnet means possess a north-south magnetic field orientation in a direction which is coplanar with a cross-sectional plane through said receiving orifices and wherein the north-south magnetic field direction orientation, moving in a common direction about the periphery of each of said receiving orifices, alternates approximately 180°
from the north-south field direction orientation of the preceding magnet whereby each nonferrous container, when placed in receiving relationship with each of said orifices, has four magnet means about its periphery. - View Dependent Claims (7, 8, 9, 10, 11, 12, 13, 14)
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15. A magnetic separation apparatus for use in assays employing solid phases comprising magnetic particles, said apparatus comprising:
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(a) base means adapted to receive microwell tray means of nonferrous composition adapted to contain said magnetic particles; and (b) a plurality of magnet means mounted on said base in spatial alignment with said microwell tray means whereby each well in said microwell tray means has adjacent thereto at least one magnet means possessing a north-south magnetic field orientation in a direction which is coplanar with a cross-sectional plane through said well and wherein each of said north-south magnetic fields of said magnet means are oriented in a common direction and wherein said magnet means are mounted in a fixed relation with respect to themselves and to said base whereby each nonferrous container, when placed in receiving relationship with each of said wells, has four magnet means about its periphery.
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16. A magnetic separation apparatus for use in assays employing solid phases comprising magnetic particles, said apparatus comprising:
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(a) base means adapted to receive microwell tray means of nonferrous composition adapted to contain said magnetic particles and having a plurality of wells; and (b) a plurality of magnet means mounted on said base in spatial alignment with said microwell tray means wherein said magnet means are mounted in a fixed relation with respect to themselves and to said base and wherein each of said magnet means possess a north-south magnetic field orientation in a direction which is coplanar with a cross-sectional plane through said microwell tray means and wherein the north-south magnetic field direction orientation, moving in a common direction about the periphery of each well in said microwell tray means, alternates approximately 180°
from the forth-south field direction orientation of the preceding magnet thereby each well of said microwell tray means, when placed in receiving relationship with said base means, has four magnet means about its periphery.
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17. A magnetic separation apparatus for use in assays employing solid phases comprising magnetic particles, said apparatus comprising:
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a. base means for receiving a nonferrous tray containing a plurality of microwells adapted to contain said magnetic particles; b. base means comprising a plurality of magnet means mounted on said base spaced on said base whereby engagement of said base and said tray results in the juxtapose placement of a plurality of magnet means about the periphery of each well wherein each of said wells is surrounded about the perhiphery by four magnet means wherein the north-south magnetic field direction orientation, moving in a common direction about the periphery of each of said wells, alternates approximately 180°
from the north-south field direction orientation of the preceding magnet.
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Specification