Microwave preservation of bioprostheses
First Claim
Patent Images
1. A method to preserve and sterilize bioprostheses comprising the following steps:
- immersing a specimen of biologic tissue in a preserving solution at room temperature (20°
C.) selected from a member of the group consisting of;
(a) 0.9% sodium chloride;
(b) 0.87% sodium chloride, 0.12% sodium phosphate dibasic, and 0.015% sodium phosphate monobasic, pH 7.3-7.6 (PBS);
(c) 20 mM Tris(hydroxymethyl)aminomethane and 0.9% sodium chloride, pH 7.3-7.6 (TRIS);
(d) 25 mM N-2-hydroxyethylpiperazine-N1 -2-ethanesulfonic acid, pH 7.3-7.6 (HEPES);
(e) 0.001-0.1% glutaraldehyde in a solution selected from a member of the group consisting of (1) 0.9% sodium chloride, (2) PBS, (3) HEPES, and (4) TRIS;
(f) a calcium chelating agent in a solution selected from a member of the group consisting of (1) 0.9% sodium chloride, (2) PBS, (3) HEPES, and (4) TRIS; and
(g) a calcium chelating agent and 0.001-0.1% glutaraldehyde in a solution selected from a member of the group consisting of (1) 0.9% sodium chloride, (2) PBS, (3) HEPES, and (4) TRIS;
exposing the immersed specimen to microwave irradiation until the final temperature of the solution is between 35° and
50°
C.; and
,storing the irradiated specimen in a cold sterile osmotically balanced solution selected from the group consisting of (1) 0.9% sodium chloride, (2) PBS, (3) HEPES, and (4) TRIS.
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Abstract
A method to preserve and sterilize biological tissues by exposing the tissue to microwave irradiation, thereby limiting tissue exposure to standard organic preservatives and retaining tissue pliability and other qualities that characterize natural, unpreserved tissue.
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Citations
14 Claims
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1. A method to preserve and sterilize bioprostheses comprising the following steps:
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immersing a specimen of biologic tissue in a preserving solution at room temperature (20°
C.) selected from a member of the group consisting of;(a) 0.9% sodium chloride; (b) 0.87% sodium chloride, 0.12% sodium phosphate dibasic, and 0.015% sodium phosphate monobasic, pH 7.3-7.6 (PBS); (c) 20 mM Tris(hydroxymethyl)aminomethane and 0.9% sodium chloride, pH 7.3-7.6 (TRIS); (d) 25 mM N-2-hydroxyethylpiperazine-N1 -2-ethanesulfonic acid, pH 7.3-7.6 (HEPES); (e) 0.001-0.1% glutaraldehyde in a solution selected from a member of the group consisting of (1) 0.9% sodium chloride, (2) PBS, (3) HEPES, and (4) TRIS; (f) a calcium chelating agent in a solution selected from a member of the group consisting of (1) 0.9% sodium chloride, (2) PBS, (3) HEPES, and (4) TRIS; and (g) a calcium chelating agent and 0.001-0.1% glutaraldehyde in a solution selected from a member of the group consisting of (1) 0.9% sodium chloride, (2) PBS, (3) HEPES, and (4) TRIS; exposing the immersed specimen to microwave irradiation until the final temperature of the solution is between 35° and
50°
C.; and
,storing the irradiated specimen in a cold sterile osmotically balanced solution selected from the group consisting of (1) 0.9% sodium chloride, (2) PBS, (3) HEPES, and (4) TRIS. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12)
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13. A method to preserve and sterilize bioprostheses comprising the following steps:
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harvesting a specimen of biologic tissue within fifteen minutes after sacrificing a donor animal; immersing said specimen of biologic tissue within fifteen minutes of harvesting in a room temperature an osmotically balanced solution selected from the group consisting of; (a) 0.9% sodium chloride; (b) PBS; (c) TRIS; (d) HEPES; (e) 0.001-0.1% glutaraldehyde in a solution selected from the group consisting of (1) 0.9% sodium chloride, (2) PBS, (3) HEPES, and (4) TRIS; (f) 1-100 mM EDTA in a solution selected from group consisting of (1) 0.9% sodium chloride, (2) PBS, (3) HEPES, and (4) TRIS; (g) 1-100 mM EDTA and 0.001-0.1% glutaraldehyde in a solution selected from the group consisting of (1) 0.9% sodium chloride, (2) PBS, (3) HEPES, and (4) TRIS; exposing the immersed specimen to microwave irradiation within fifteen minutes of immersion in said preserving solution for 1-50 seconds until the temperature of the solution is between 35° and
50°
C.; and
,storing the irradiated specimen within thirty seconds of irradiation at 4°
C. in a sterile osmotically balanced solution selected from the group consisting of (1) 0.9% sodium chloride, (2) PBS, (3) HEPES, and (4) TRIS. - View Dependent Claims (14)
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Specification