Process for production of pyrrolo-quinoline quinone

• US 4,994,382 A
• Filed: 05/26/1989
• Issued: 02/19/1991
• Est. Priority Date: 05/29/1984
• Status: Expired due to Fees

• Current Assignee: UBE Industries Limited (UBE Corporation)
• Original Assignee: UBE Industries Limited (UBE Corporation)
• Inventors: Ameyama, Minoru, Adachi, Osao
• Primary Examiner(s): Lilling, Herbert J.
• Application Number:
  US07/357,668
• Time in Patent Office: 634 Days
• Field of Search: 435/119, 435/118, 435/133, 435/247, 435/822, 435/874, 435/253.3, 435/252.34
• US Class Current: 435/119
• CPC Class Codes: C12P 17/182 Heterocyclic compounds containing nitrogen atoms as the only ring heteroatoms in the condensed system alloxazine or isoalloxazine, e.g. riboflavine C12P25/00
• CPC Class Codes: Y10S 435/822 using bacteria or actinomycetales
• CPC Class Codes: Y10S 435/874 Pseudomonas

Abstract

There are disclosed a new process for production of pyrrolo-quinoline quinone, comprising culturing a bacterium belonging to the genus Paracoccus, Protaminobacter or Pseudomonas and capable of producing pyrrolo-quinoline quinone in a culture medium to produce the pyrrolo-quinoline quinone in the cultured broth, and recovering the pyrrolo-quinoline quinone from the cultured broth; and a new process for production of pyrrolo-quinoline quinone, comprising culturing a bacterium belonging to the genus Paracoccus, Protaminobacter or Pseudomonas and capable of producing the pyrrolo-quinoline quinone in a culture medium to form cultured cells, separating the cells from the cultured broth, resuspending the separated cells in a reaction medium containing precursors of the pyrrolo-quinoline quinone, incubating the reaction medium to produce the pyrrolo-quinoline quinone, and recovering the pyrrolo-quinoline quinone from the reaction medium.

Claims

1. A process for producing pyrrolo-quinoline quinone, comprising culturing a bacterium Pseudomonas N1-1 (FERM BP-775) in a culture medium containing methanol at a concentration of from 0.5% to 5.0% by weight under conditions in which said bacterium can grow to produce said pyrrolo-quinoline quinone in the cultured medium, and recovering at least about 26.1 ug/ml of the pyrrolo-quinoline quinone from the cultured medium.

2. A process for producing pyrrolo-quinoline quinone, comprising culturing a bacterium Pesudomonas N1-1 (FERM BP-775) in a culture medium to form cultured cells, separating the cells from the cultured broth, resuspending the separated cells in a reaction medium comprising a carbon source at a concentration of from 0.5% to 3.0% by weight, wherein the carbon source is selected from the group consisting of methanol, ethanol, mannitol, fructose and glucose, and a nitrogen source at a concentration of from 0.05% to 0.5% by weight wherein the nitrogen source is selected from the group consisting of glutamic acid, aspartic acid, alanine, ornithine, tyrosine and 3,4-dihydroxyphenylalanine (DOPA), incubating the reaction medium to produce the pyrrolo-quinoline quinone, and recovering at least about 26.1 ug/ml of the pyrrolo-quinoline quinone from the reaction medium.