Biological sensors

• US 4,997,278 A
• Filed: 08/16/1988
• Issued: 03/05/1991
• Est. Priority Date: 08/22/1987
• Status: Expired due to Term

• Current Assignee: GE Healthcare Bio-Sciences AB (General Electric Company)
• Original Assignee: Amersham International Plc (General Electric Company)
• Inventors: Charles, Stephen A., Finlan, Martin F., Midgley, John E. M., Irlam, James C.
• Primary Examiner(s): Rosenberger, Richard A.
• Assistant Examiner(s): PHAM, HOA Q
• Application Number:
  US07/232,650
• Time in Patent Office: 931 Days
• Field of Search: 356/127-129, 356/132, 356/135, 356/136, 356/318, 356/317, 356/311, 356/445
• US Class Current: 356/128
• CPC Class Codes: G01N 21/553 and using surface plasmons fluorescence excitation G01N21/648; enhanced Raman G01N21/658
• CPC Class Codes: G01N 33/54373 involving physiochemical end-point determination, e.g. wave-guides, FETS, gratings

Abstract

A sensor uses the principle of surface plasmon resonance (SPR) to monitor the progress of the reaction between a sample and a sensitive layer (for example an antibody layer). The layer is applied to the rear surface of a metallic film formed on the surface of an optically transmissive component in the form of a hemicylindrical lens and slide. Collimated light from a source is applied via a lens which focuses the incoming beam to a focus at a point to form a fan-shaped spread of light incident at the point. The light is internally reflected at the point, and emerges from the component to be applied to a dectector array which latter is electronically scanned. The angle of incidence of the light at the point is such as to span that angle which gives rise to surface plasmon resonance, together with a range of angles thereabout so that the progress of the resonant condition, as the reaction between the sample and the sensitive layer proceeds, can be monitored.

Claims

1. A sensor for use in biological, biochemical or chemical testing, said sensor comprising a block of material transparent to electromagnetic radiation, a layer of metallic material applied to at least part of a first surface of said block, a layer of sensitive material applied to the metallic layer, means for introducing onto the sensitive layer so as to react therewith a sample to be analyzed, a source of electromagnetic radiation, said radiation being directed into said transparent block in such a way as to be internally reflected off said part of said surface, means for converging said radiation onto said part of said surface in such a way that the incoming beam is a convergent fan-shaped beam and spans a range of angles of incidence including that which causes surface plasmon resonance to occur, the characteristics of which resonance are dependent upon the reaction between the sample and the sensitive layer, and detector means positioned to receive the internally reflected beam for detecting the characteristics of the resonance that are dependent upon the reaction between the sample and the sensitive layer.

2. A sensor as claimed in claim 1 wherein the radiation from said source enters the block through a second, curved, surface.

3. A sensor as claimed in claim 2 wherein the center of curvature of said second, curved, surface lies on said first surface.

4. A sensor as claimed in claim 2 wherein said second surface is spherical.

5. A sensor as claimed in claim 2 wherein the input beam of electromagnetic radiation enters the block in a direction orthogonal to a tangent to the second, curved, surface at the point of entry.

6. A sensor as claimed in claim 2 wherein said detector means is positioned externally of said block, and wherein the internally reflected beam emerges from said block through a third surface of the block, said third surface being curved.

7. A sensor as claimed in claim 6 wherein the center of curvature of said third surface lies on said first surface.

8. A sensor as claimed in claim 2 wherein said transparent block takes the form of a hemisphere whose flat surface is said first surface and whose spherical surface includes said second surface, and wherein said part of said first surface is positioned at the center of the first surface.

9. A sensor as claimed in claim 2 wherein said transparent block takes the form of a semicylinder whose flat surface is said first surface and whose curved surface includes said second surface and wherein said part of said first surface is positioned on the longitudinal central axis of said first surface.

10. A sensor as claimed in claim 2 wherein said transparent block takes the form of a truncated hemisphere one of whose flat surfaces is said first surface and whose spherical surface includes said second surface, and wherein said part of said first surface is positioned at the center of the first surface.

11. A sensor as claimed in claim 2 wherein said transparent block takes the form of a truncated semicylinder one of whose flat surfaces is said first surface and whose curved surface includes said second surface, and wherein part of said first surface is positioned on the longitudinal central axis of said first surface.

12. A sensor as claimed in claim 1 wherein the detector means takes the form of a large-area detector positioned to collect the whole emergent beam.

13. A sensor as claimed in claim 1 wherein the detector means takes the form of an array of angularly spaced detectors positioned to collect the whole emergent beam.

14. A sensor as claimed in claim 1 wherein said sensitive layer takes the form of an antibody layer to be reacted with a sample containing a corresponding antigen.