Assaying for a biologically active component

• US 4,997,526 A
• Filed: 03/19/1985
• Issued: 03/05/1991
• Est. Priority Date: 03/19/1985
• Status: Expired due to Fees

• Current Assignee: EIC Laboratories, Inc.
• Original Assignee: EIC Laboratories, Inc.
• Inventors: Robblee, Lois S.
• Primary Examiner(s): Nguyen, Nam X.
• Application Number:
  US06/713,469
• Time in Patent Office: 2,177 Days
• Field of Search: 204/1 T, 204/1 E, 204/403, 204/412, 436/806, 435/817
• US Class Current: 205/780.5
• CPC Class Codes: G01N 33/5438 Electrodes
• CPC Class Codes: Y10S 436/806 Electrical property or magnetic property

Abstract

A liquid mixture is assayed for a biologically active component that undergoes a reduction or oxidation reaction within a specific voltage range by immobilizing at an electrically conductive surface, a binding partner for the component, exposing the surface to the component to permit complexing to occur, applying a voltage to the surface in a range which will cause the component to undergo an oxidation or reduction reaction, and measuring the resulting current as an indication of the presence of the component.

Claims

1. A method of assaying a liquid mixture for a biologically active component that is characterized by undergoing a reduction or oxidation reaction in response to the application of a voltage level that is within the range of voltage levels over which said reaction occurs, said method comprising:

providing an electrically conductive surface,

immobilizing at said surface a binding partner capable of selectively complexing with said component,

exposing said surface to said mixture;

applying a voltage within said range between said surface and said mixture to cause said component that has complexed with said immobilized binding partner to undergo said reaction;

varying said voltage with time according to a predetermined profile; and

measuring a current that results from said reaction caused by applying said voltage, as an indication of the presence of said component in said liquid mixture.

2. The method of claim 1 wherein said profile is a linear voltage sweep with time.

3. The method of claim 1 or 2 wherein a plurality of complexing sites are immobilized at said conductive surface, and said exposing step comprises exposing said conductive surface to said mixture for a time that allows said component to complex at some but not all of said sites.

4. The method of claim 1 or 2 wherein after said exposing step said surface is exposed to a blank electrolyte for said measurement.

5. A method of assaying a liquid mixture for a biologically active component that is characterized by undergoing a reduction or oxidation reaction in response to the application of any voltage level that is within a range of voltage levels over which said reaction occurs, said method comprising:

providing an electrically conductive surface,

immobilizing at said surface a binding partner capable of selectively complexing with said component,

exposing said surface to said mixture;

applying a voltage within said range between said surface and said mixture to cause said component that has complexed with said immobilized binding partner to undergo said reaction,

varying said voltage in according with a double differential pulse technique, and

measuring a current that results from said reaction caused by applying said voltage, as an indication of the presence of said component in said liquid mixture.

6. The method of claim 5 wherein a plurality of complexing sites are immobilized at said conductive surface, and said exposing step comprises exposing said conductive surface to said mixture for a time that allows said component to complex at some but not all of said sites.

7. The method of claim 5 or 6 wherein after said exposing step said surface is exposed to a blank electrolyte for said measurement.