Weighted collagen microsponge for immobilizing bioactive material
First Claim
1. A weighted collagen microsponge for immobilizing bioactive materials in motive bioreactor systems, said microsponge comprising a porous, biocompatible, biostable, insoluble highly crosslinked collagen matrix containing an inert weighting material, said collagen matrix having an open to the surface pore structure with an average pore size in the range of from about 1 micron to about 150 microns, the pores of said matrix occupying from about 70 to about 98% by volume of the microsponge, said microsponge also having an average particle size of from about 100 to about 1000 microns and a specific gravity above about 1.05.
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Abstract
Weighted collagen microsponges having a highly crosslinked collagen matrix are described suitable for use in culturing organisms in motive reactor systems. The microsponges have an open to the surface pore structure, and pore sizes and volumes suitable for immobilizing a variety of bioactive materials. The microsponges also have an average particle size in the range of about 100 to about 1000 microns and a specific gravity above about 1.05.
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20 Claims
- 1. A weighted collagen microsponge for immobilizing bioactive materials in motive bioreactor systems, said microsponge comprising a porous, biocompatible, biostable, insoluble highly crosslinked collagen matrix containing an inert weighting material, said collagen matrix having an open to the surface pore structure with an average pore size in the range of from about 1 micron to about 150 microns, the pores of said matrix occupying from about 70 to about 98% by volume of the microsponge, said microsponge also having an average particle size of from about 100 to about 1000 microns and a specific gravity above about 1.05.
- 10. A bioreactor system comprising a reactor vessel having aseptically sealed therein a plurality of sterilized weighted collagen microsponges for immobilizing bioactive materials in motive bioreactor systems, said microsponges comprising a porous, biocompatible, biostable, insoluble highly crosslinked collagen matrix containing an inert weighting material, said collagen matrix having an open to the surface pore structure with an average pore size in the range of from about 1 to about 150 microns, the pores of said matrix occupying from about 70 to about 98% by volume of the microsponge, said microsponge also having an average particle size of from about 100 to about 1000 microns and a specific gravity of above about 1.05.
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13. A process for performing a bioreaction comprising immobilizing a bioactive material in weighted collagen microsponges comprising a porous, biocompatible, biostable, insoluble highly crosslinked collagen matrix containing an inert weighting material, said collagen matrix having an open to the surface pore structure with an average pore size in the range of from about 1 micron to about 150 microns, the pores of said matrix occupying from about 70 to about 98% by volume of the microsponge, said microsponge also having an average particle size of from about 100 to about 1000 microns and a specific gravity above about 1.05;
- retaining the microsponges having said immobilized bioactive material in a suitable reactor vessel;
passing a liquid reagent stream into said reactor in direct contact with said microsponges;
agitating the mixture of said microsponges and said reagent stream and recovering the biochemical reaction products from said reactor. - View Dependent Claims (14, 15, 16, 17, 18, 19, 20)
- retaining the microsponges having said immobilized bioactive material in a suitable reactor vessel;
Specification