Purified ciliary neurotrophic factor

• US 4,997,929 A
• Filed: 09/08/1989
• Issued: 03/05/1991
• Est. Priority Date: 01/05/1989
• Status: Expired due to Term

• Current Assignee: Regeneron Pharmaceuticals Incorporated, Znidarcic Marijana Personal Representative of The Estate of Mismer Drzislav Deceased
• Original Assignee: Synergen, Inc. (Oracle Corporation)
• Inventors: Collins, Franklin D., Lin, Leu-Fen
• Primary Examiner(s): JONES, WILLIAM G
• Application Number:
  US07/404,533
• Time in Patent Office: 543 Days
• Field of Search: 435/69.1, 435/69.4, 435/172.3, 435/240.2, 435/320, 935/70, 935/60, 530/412, 530/417, 530/418, 530/419, 536/27
• US Class Current: 435/365.1
• CPC Class Codes: A61K 38/00 Medicinal preparations containing peptides peptides containing beta-lactam rings A61K31/00; cyclic dipeptides not having in their molecule any other peptide link than those which form their ring, e.g. piperazine-2,5-diones, A61K31/00; ergot alkaloids of the cyclic peptide type A61K31/48; containing macromolecular compounds having statistically distributed amino acid units A61K31/74; medicinal preparations containing antigens or antibodies A61K39/00; medicinal preparations characterised by the non-active ingredients, e.g. peptides as drug carriers, A61K47/00
• CPC Class Codes: C07K 14/475 Growth factors; Growth regulators
• CPC Class Codes: C07K 16/22 against growth factors ; against growth regulators

Abstract

A ciliary neurotrophic factor (CNTF), particularly sciatic nerve CNTF(SN-CNFT) is claimed. The SN-CNTF described herein is a single protein species and has a specific activity that increased to greater than 25,000-fold from crude extract.

Amino acid data for this SN-CNTF is also provided. In addition, methods for using this data for providing SN-CNTF probes and for screening cDNA and genomic libraries are also provided. Recombinant-DNA methods for the production of SN-CNTF are described.

Nucleic acid sequences encoding rabbit and human CNTF are provided. A recombinant expression system is provided for producing biologically active CNTF.

Claims

1. The nucleic acid sequence encoding rabbit SN-CNTF as set forth in FIG. 11.

2. The nucleic acid sequence encoding human CNTF as set forth in FIG. 12.

3. A recombinant expression system for producing biologically active SN-CNTF comprised of an animal cell transfected with a vector containing a nucleic acid sequence encoding for SN-CNTF.